Supplementary material
Article title: Evolution of cadmium tolerance and associated costs in a Gammarus fossarum population inhabiting a low-level contaminated stream
Authors: Vigneron A§, Geffard O§, Coquery M§, François A§, Quéau H§, Chaumot A§£
Author affiliations:
§ Irstea, UR MALY Milieux aquatiques, écologie et pollutions, centre de Lyon-Villeurbanne, F-69626 Villeurbanne, France.
£ Corresponding author: Arnaud Chaumot
address: Irstea, UR MALY Milieux aquatiques, écologie et pollutions, centre de Lyon-Villeurbanne, 5 rue de la doua – CS70077, F-69626 Villeurbanne, France.
Telephone number: 33 4 72208788 Fax number: 33 4 78477875
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Supplementary Table 1. Characterization of the bioavailable metallic contamination in the six study sites. Mean accumulated concentrations of caged gammarids (transplanted for 7 days from one source population) are presented. Concentrations of metals, limits of quantifications (LQ) and threshold values (TV) of contamination are expressed in µg g-1 dw (dry weight). Detailed protocol and determination of TV are reported by Besse et al. (2013).
Supplementary Table 2. Size of gammarid males used for each experiment.
Supplementary Table 3. Dissolved Cd concentration in water of the Ardi site on different dates.
Supplementary Figure 1. Complementary Cd toxicity test with the G. fossarum populations (July 2013). One week acclimatized males were exposed to 160 µg Cd L-1 (nominal concentration). Small dots represent replicates; large dots represent the mean survival rate; vertical segments link minimum and maximum survival rates.
Supplementary Table 1. Characterization of the bioavailable metallic contamination in the six study sites. Mean accumulated concentrations of caged gammarids (transplanted for 7 days from one source population) are presented. Concentrations of metals, limits of quantifications (LQ) and threshold values (TV) of contamination are expressed in µg g-1 dw (dry weight). Detailed protocol and determination of TV are reported by Besse et al. (2013).
Supplementary Table 2. Size of gammarid males used for each experiment.
Supplementary Table 3. Dissolved Cd concentration in water of the site inhabited by the Ardi population on different dates.
Supplementary Figure 1. Complementary Cd toxicity test with the G. fossarum populations (July 2013). One week acclimatized males were exposed to 160 µg Cd L-1 (nominal concentration). Small dots represent replicates; large dots represent the mean survival rate; vertical segments link minimum and maximum survival rates.