Supplementary Figures:
Supplementary Figure 1: MOMP and EB-specific IgG isotypes following chlamydial infection or immunization with MOMP
IgG produced in response to male and female mouse chlamydial infection (12 w infection) or MOMP-immunization (CT or Freunds adjuvant). Antigen-specific IgG isotypes were determined by ELISA screening with MOMP antigen (A), or UV-inactivated EBs (B) (n = 8-10).
Supplementary Figure 2: Chlamydia spp. accumulate FcRn Independent of Antibody
C. muridarum infected mECap18 cells were stained for colocalization of chlamydia and FcRn at various time points (A) or in the presence of chemical inhibitors (B). (C) HEC1A cells were infected with C. trachomatis D or L2 for 48h and stained for chlamydia and FcRn. White arrows = inclusions, yellow arrows = co-localization. Scale bar = 2.5 µm.
(A) mECap18 cells were infected with C. muridarum fixed and co-stained for chlamydial MOMP and mouse FcRn. (B) mECap18 cells infected for 24 hours including DMSO, cyclohexamide, or nocodazole. (C) HEC1A infected with C. trachomatis serovars D or L2 for 48 hours co-stained for EBs and human FcRn.
Supplementary Figure 3: Antigen-Specific Serum IgA at Euthanasia
Serum antigen-specific IgA endpoint titers of WT and β2m -/- mice immunized with corresponding antigen at euthanasia were determined by direct ELISA.
Supplementary Figure 4: Chlamydial Burden in the Testes
C57Bl/6 male mice were infected with 106 IFUs via the meatus glans and the testicular chlamydial burden determined weekly for 7 weeks by culture and fluorescence microscopy. Error bars indicate mean ± SEM (n = 5 mice per time point).
Supplementary Figure 5: β2m plays a Supportive but Non-Essential Role in Recovery from Respiratory Chlamydial Infection
WT and β2m -/- mice were intranasally infected with 103 IFUs of C. muridarum and weighed daily until recovery. (A) Percent weight loss of mice following intranasal challenge. (B) Net percentage loss (magnitude) of weight over 15 days of respiratory infection (n = 5 per group).