Supplementary Table 4. BLAST results of downloaded 16S rDNA sequences and its insilico amplified product using primers 265F1 and 265R 1 through Fast PCR version 5.4.

220 bp / Accession No. / Homology / 16S rDNA / Accession No. / Homology
Bacillus aquimaris strain TF-12 16S ribosomal RNA, partial sequence / NR_025241.1 / 100% / Bacillus aquimaris strain TF-12 16S ribosomal RNA, partial sequence / NR_025241.1 / 100%
Bacillus asahii strain ma001 / GQ150535.1 / 100% / Bacillus asahii strain MA001 16S ribosomal RNA, partial sequence / NR_024817.1 / 100%
Bacillus cohnii 16S rRNA gene, strain US147 / AM287289.1 / 100% / Bacillus cohnii strain DSM 6307 16S ribosomal RNA, complete sequence / NR_026138.1 / 100%
Bacillus firmus strain UST981101-006 16S ribosomal RNA gene, partial sequence / FJ188300.1 / 100% / Bacillus firmus strain IAM 12464 16S ribosomal RNA, partial sequence / NR_025842.1 / 100%
Bacillus humi strain LMG 22167 16S ribosomal RNA, partial sequence / NR_025626.1 / 100% / Bacillus humi strain LMG 22167 16S ribosomal RNA, partial sequence / NR_025626.1 / 100%
Bacillus shackletonii strain LMG 18435 16S ribosomal RNA, partial sequence / NR_025373.1 / 100% / Bacillus shackletonii strain LMG 18435 16S ribosomal RNA, partial sequence / NR_025373.1 / 100%
Bacillus sporothermodurans 16S rRNA type III gene, partial sequence / U49080.1 / 100% / Bacillus sporothermodurans strain M215 16S ribosomal RNA, partial sequence / NR_026010.1 / 100%
Bacillus subtilis strain Sd2B10(R) 16S ribosomal RNA gene, partial sequence / GQ285101.1 / 100% / Bacillus subtilis subsp. subtilis strain DSM 10 16S ribosomal RNA, partial sequence / NR_027552.1 / 100%
Bacillus mojavensisJF-2 16S ribosomal RNA gene, partial sequence / AY436360.1 / 100% / Bacillus mojavensis strain IFO15718 16S ribosomal RNA, partial sequence / NR_024693.1 / 100%
Bacillus horikoshii strain NBSL27 16S ribosomal RNA gene, partial sequence / FJ984530.1 / 100% / Bacillus horikoshii strain NBSL27 16S ribosomal RNA gene, partial sequence / FJ984530.1 / 100%
Paenibacillus cookii strain B39 16S ribosomal RNA gene, partial sequence / DQ153950.2 / 100% / Paenibacillus cookii strain LMG 18419 16S ribosomal RNA, partial sequence / NR_025372.1 / 100%
Bacillus boroniphilus strain S8-01 16S ribosomal RNA gene, partial
sequence / EU620409.1 / 100% / Bacillus boroniphilus strain S8-01 16S ribosomal RNA gene, partial sequence / EU620409.1 / 100%
Bacillus thuringiensis partial 16S rRNA gene, isolate CCM15B
Bacillus weihenstephanensis partial 16S rRNA gene, isolate CCM16B / FN433029.1
FN433021.1 / 100%
100% / Bacillus weihenstephanensis strain DSM11821 / NR_024697.1 / 100%
Bacillus carboniphilus strain JCM9731 16S ribosomal RNA, partial
sequence / NR_024690.1 / 100% / Bacillus carboniphilus strain JCM9731 16S ribosomal RNA, partial sequence / NR_024690.1 / 100%
Bacillus flexus strain WT019 16S ribosomal RNA gene, partial sequence / GQ152128.1 / 100% / Bacillus flexus strain IFO15715 16S ribosomal RNA, partial sequence / NR_024691.1 / 100%
Brevibacillus thermoruber gene for 16S rRNA, partial sequence, strain / AB112722.1 / 100% / Brevibacillus thermoruber strain BT2 16S ribosomal RNA, partial sequence / NR_026514.1 / 100%
Bacillus agaradhaerens strain DSM 8721 16S ribosomal RNA, partial seq / NR_026142.1 / 100% / Bacillus agaradhaerens strain DSM 8721 16S ribosomal RNA, partial sequence / NR_026142.1| / 100%
Bacillus pseudofirmus gene for 16S rRNA, partial sequence, clone / AB201799.1 / 100% / Bacillus pseudofirmus strain DSM 8715 16S ribosomal RNA, complete sequence / NR_026139.1 / 100%
Anoxybacillus voinovskiensis strain TH13 16S ribosomal RNA, partial se / NR_024818.1 / 100% / Anoxybacillus voinovskiensis strain TH13 16S ribosomal RNA, partial sequence / NR_024818.1 / 100%
Alicyclobacillus pomorum strain 3A 16S ribosomal RNA, partial sequence / NR_024801.1 / 100% / Alicyclobacillus pomorum strain 3A 16S ribosomal RNA, partial sequence / NR_024801.1 / 100%

Supplementary Figure1.Position of the forward primer (265F) in the 16S rDNA sequence of Bacillus subtilis.

Supplementary Figure2.Position of the Reverse primer (265R) in the 16S rDNA sequence of Bacillus subtilis.

Supplementary Figure3. ARDRA pattern obtained with Alu I digestion from lane 28-47 with Marker 100bp. Lanes indicates 1st lane100bp Marker, lanes 28- B.aminovorans,29- B.mycoides,30- B. sporothermodurans,31- B.megaterium,32- B. firmus,33- B. laevolacticus,34- Sporosarcina globisporus,35- B. cereus,36- B.amyloliquifaciens,37- B. firmus,38- Sporosarcina pasteurii,39- Geobacillus stearothermophilus,40- Paenibacillus larvae,41- Paenibacillus polymyxa,42- B. oleronius,43- B. fumarioli,44- B.pichinotyi,45- Paenibacillus lactis,46- B. sporothermodurans,47- B.djibelorensis& Last lane 100 bp Marker.

Supplementary Figure4. ARDRA pattern obtained with Alu I digestion from lane 47-56 with Marker 100bp. Lanes indicates 1stlane100 bp Marker, 48- B.laterosporus, 49- B.ginsengi, 50- B.senegalensis, 51- B. asahii, 52- B. subtilis, 53- B.altitudinus, 54- Virgibacillus sp, 55-B. arsenicus, 56-Geobacillus stearothermophilus.

Suplimentary Figure 2.

Suplimentary Figure 3.

Suplimentary Figure 4.