Supplementary figure 4a
Alignment of B1334-like sequences amplified from cDNA of plants with (+B) and without (0B) B chromosomes. Single nucleotide polymorphisms are highlighted in red. Genbank sequence AF175285 shows the highest similarity.Sequences B1334-1 - 9 are sequences obtained via cDNA-AFLP and sequencesB1334-10 - 23 are sequences obtained via RT-PCR.
* 20 * 40 * 60 * 80 * 100
AF175285^h : CATCATTCAAAAACATCACAACCC-GA-CTACG-GGTGAAGATCCA-AAAGAGAGATACCAAAACA-ACA-TAAAGCCAC--AGGCCAGGATC-GAATCCTAGTA : 98
B1334-1 +B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-2 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGTAGGATC-GAATCCTAGTA : 96
B1334-3 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC-AGGGGCAGGATC-GAATCCTAGTA : 97
B1334-4 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-5 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-6 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-7 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-8 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B8134-9 +B: CATCATACAAAA-CATCACAACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 96
B1334-10 +B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 97
B1334-11 +B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 97
B1334-12+B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACA-ACA-TAAAGCCAC-AGGGCAGAGATC-GAACCCTAGTA : 98
B1334-13+B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAAGAAAGATACCAAAACT-ACA-TAAAGCCAC--AGGGCAGGATC-GAATCCTAGTA : 97
B1334-14 +B: CATCATACAAAA-CATCACGACCC-GA-CTACGTGGTGAAGAACCATAAAGAAAGATACCGAAACT-ACA-TAAAGCCAGCAGGGTCAGGATC-GAATCCTAGTA : 101
B1334-15+B: CATCATACAAAA-CATCACGACCC-GATCTACG-GGTGAAGAACCATAAAGAAAGATACCAAAACT-ACAGTAAAGCCAGCAGGGCGAGGATCTGAACCCTAGTA : 103
B1334-16+B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AAGGAAAGATACCAAAACA-ACA--AAAGCCAT--AGGTCAGGATC-GGATCCTAGTA : 96
B1334-17+B: CATCATACAAAA-CATCACGACCCAGATCTACG-GGTGAAGAACCA-AAGGAAAGATACCAAAACT-ACA--AAAGCCAT--AGGTCAGGATCGGATCCTTAGTA : 99
B1334-180B: CATCATACAAAA-CATCACGACCC-GATCTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACATACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 98
B1334-190B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACA-ACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 96
B1334-20 0B: CATCATACAAAA-CATCACGACCC-GATCTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACA-ACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 97
B1334-21 0B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACA-ACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 96
B1334-22 0B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACA-ACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 96
B1334-23 0B: CATCATACAAAA-CATCACGACCC-GA-CTACG-GGTGAAGAACCA-AA-GAAAGATACCAAAACA-ACA-TAAGACCAC--AGGCCAAGATC-GAATCCTAGTA : 96
Supplementary figure 4a. continued
* 120 * 140 * 160
AF175285^h : GAAACCA-CTACTGAACATCGAA-ACGA-CACAAACATACGGCTACTCTCTTCA-TG-GAACTCCTGG: 161
B1334- 1 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTC----: 155
B1334- 2 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B1334- 3 +B: GAAACCA-CTACTGAACGTCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 160
B1334- 4 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTCCA-TCGGA-CTCCCG-: 158
B1334- 5 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B1334- 6 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B1334- 7 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B1334- 8 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B8134- 9 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TC-GGACTCCCGG: 159
B1334- 10 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 160
B1334- 11 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 160
B1334- 12 +B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 161
B1334- 13 +B: GAAACCATCTACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 161
B1334- 14 +B: GAAACCAT-TACTGAACATCGAATACGTGCACAAGCAAACAGCCACGATCTTCAATCGGATCTCCCGG: 168
B1334- 15 +B: GAAACCATCTACTGAACATCGAATACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 168
B1334- 16 +B: GAAACCAC-TACTGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 159
B1334- 17 +B: GAAACCACTACATGAACATCGAA-ACGT-CACAAGCAAACAGCCACGATCTTCA-TCGGA-CTCCCGG: 163
B1334- 18 0B: GAAACCATCTATTGAACATCGAA-ACGTGCACAAGCA-ACAGCCACGATCTTCA-TC-GGACTCCCGG: 162
B1334- 19 0B: GAAACCATCTACTGAACATCGAA-ACGT-CACAAGCA-ACAGCCACGATCTTCA-TCTGGACTCCCGG: 160
B1334- 20 0B: GAAACCATCTACTGAACATCGAA-ACGT-CACAAGCA-ACAGCCACGATCTTCA-TC-GGACTCCCGG: 160
B1334- 21 0B: GAAACCA-CTATTGAACATCGAA-ACGT-CACAAGCA-ACAGCCACGATCTTCA-TC-GGACTCCCGG: 158
B1334- 22 0B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCA-ACAGCCACGATCTTCA-TC-GGACTCCCGG: 158
B1334- 23 0B: GAAACCA-CTACTGAACATCGAA-ACGT-CACAAGCA-ACAGCCACGATCTTCA-TC-GGACTCCCGG: 158
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