Supplementary Fig. 1 ACAD Domain Alignment of IBR3 and IBR3 Homologs. IBR3 Was Compared

Supplementary Fig. 1 ACAD domain alignment of IBR3 and IBR3 homologs. IBR3 was compared to similar proteins from Arabidopsis (At), rice (Os), chicken (Gg), humans (Hs), Tetrahymena (Tt), mouse (Mm), Caenorhabditis elegans (Ce), rat (Rn), pig (Ss), and the bacterium Megasphaera elsdenii (Me), using only the ACAD domain for the multidomain proteins and the ACAD domain without the predicted mitochondrial targeting sequences for the other proteins (IVD, SCAD, and MCAD). The alignment was generated in the MegAlign program (DNAStar) using the Clustal W method. Amino acid residues identical in at least seven sequences are shaded in black; chemically similar amino acids are shaded in grey. Hyphens indicate gaps introduced to maximize alignment. The locations of the mutations in ibr3-1 and ibr3-2 are indicated above the sequence by arrows, and the catalytic glutamate residues in Ss MCAD and Hs IVD (Kim and Miura 2004) are indicated below the sequence. This alignment was used to generate the phylogenetic tree shown in Figure 7.

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