Supplementary data 1. Primers used for amplification and sequencing of rotavirus genes

Gene / Primer / Primer Sequence (5’- 3’) / Position / Reference
VP1 / K-VP1F / GGC TAT TAA AGC TGT ACA ATG / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-VP1R / GGT CAC ATC TAA GCA CTC TAA / 3282–3302, - / (Mijatovic-Rustempasic et al., 2011)
V1Rint1 / ACT CTG TCT TCA ATT GGT CTA / 1049–1069, - / (Mijatovic-Rustempasic et al., 2011)
V1Fint1 / TGA TTG AYG CAG CAT TAG AC / 1034–1054, + / (Mijatovic-Rustempasic et al., 2011)
V1Rint2 / GAC TGT AGA TAC CAA TGC TT / 2018–2037, - / (Banyai et al., 2009)
V1Fint2 / GGT HCA AGA TGT GTC AAA GCA / 1959–1979, + / (Mijatovic-Rustempasic et al., 2011)
VP2 / K-VP2F / GGC TAT TAA AGG CTC AAT GGC / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-VP2R / GGT CAT ATC TCC ACA GTG G / 2699–2717, - / (Mijatovic-Rustempasic et al., 2011)
V2Rint1 / GGT CTA ATA TAT CGR GCT GTT / 922–942, - / (Mijatovic-Rustempasic et al., 2011)
V2Rint2 / CTG CAT TAA TGC TTC CAT TAA / 1598–1618, - / (Banyai et al., 2009)
V2Fint1 / GCA GAT ATG AGA CAA CAA GTT / 716–736, + / (Mijatovic-Rustempasic et al., 2011)
V2Fint2 / GGC TTG TAT AAC TAT GAA YAT / 1720–1740, + / (Mijatovic-Rustempasic et al., 2011)
VP3 / K-VP3F / GGC TAT TAA AGC AGT ACY AGT / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-VP3R / GGT CAC ATC YTG ACT AGT GT / 2572–2591, - / (Mijatovic-Rustempasic et al., 2011)
V3Rint1 / ATG GAT CCC ATG TCT CAA ATG / 957–977, - / (Mijatovic-Rustempasic et al., 2011)
V3Fint1 / TAC ATT TGA RAC ATG GGA TCC / 955–975, + / (Mijatovic-Rustempasic et al., 2011)
V3Rint2 / GAA TAT GAR GTC GCA GAC TCT / 1786–1806, S / (Mijatovic-Rustempasic et al., 2011)
V3Fint2 / TTA CAT CAT ATG ATG GTT GT / 1614–1633, + / (Mijatovic-Rustempasic et al., 2011)
V3Fint3 / TCA GTT TCA GGA CAT GTY TA / 1871–1890, + / (Mijatovic-Rustempasic et al., 2011)
VP4 / K-VP4F / GGC TAT AAA ATG GCT TCR CTC / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-VP4R / GGT CAC ATC CTC AAT AGC GTT / 2339–2359, - / (Mijatovic-Rustempasic et al., 2011)
Con2 / ATT TCG GAC CAT TTA CC / 887–868, S / (Banyai et al., 2004)
Con3 / TTG CTT CGC TCA TTT ATA GAC A / 11–32, + / (Banyai et al., 2004)
VP6 / JRG7 / GGC TTT AAA ACG AAG TCT TC / 1–20, + / (Kerin et al., 2007)
JRG8 / GGT CAC ATC CTC TCA CTA CAT / 1336–1356, - / (Kerin et al., 2007)
VP7 / Beg9 / GGC TTT AAA AGA GAG AAT TTC CGT CTG G / 1-28 / (Gouvea et al., 1999)
End9 / GGT CAC ATC ATA CAA TTC TAA TCT AAG / 1062-1036 / (Gouvea et al., 1999)
NSP1 / K-NSP1F / GGC TTT TTT TWT GAA AAG TCT / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-NSP1R / GGT CAC ACT TTT TTA TGC TGC / 1548–1568, - / (Mijatovic-Rustempasic et al., 2011)
N1Rint1 / TCT YAA ATC ATT CCA TAC AG / 1023–1042, - / (Mijatovic-Rustempasic et al., 2011)
N1Fint1 / GTY TCH ACT AGA TGT AG / 878–894, + / (Mijatovic-Rustempasic et al., 2011)
NSP2 / K-NSP2F / GGC TTT TAA AGC GTC TCA GTC / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-NSP2R / GGT CAC ATA AGC GCT TTC TAT / 1039–1059, - / (Mijatovic-Rustempasic et al., 2011)
NSP3 / K-NSP3F / GGC TTT TAA TGC TTT TCA GTG G / 1–22, + / (Mijatovic-Rustempasic et al., 2011)
K-NSP3R / GGT CAC ATA ACG CCC CTA TAG / 1064–1084, - / (Mijatovic-Rustempasic et al., 2011)
NSP4 / K-NSP4F / GGC TTT TAA AAG TTC TGT TCC / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-NSP4R / GGT CAC ATT AAG ACC YTT CCT / 731–751, - / (Mijatovic-Rustempasic et al., 2011)
NSP5 / K-NSP5F / GGC TTT WAA AGC GCT ACA GTG / 1–21, + / (Mijatovic-Rustempasic et al., 2011)
K-NSP5R / GGT CAC AAA ACG GGA GTG G / 646–664, - / (Mijatovic-Rustempasic et al., 2011)

Banyai, K., Bogdan, A., Domonkos, G., Kisfali, P., Molnar, P., Toth, A., Melegh, B., Martella, V., Gentsch, J. R., Szucs, G., 2009. Genetic diversity and zoonotic potential of human rotavirus strains, 2003-2006, Hungary. J Med Virol. 81, 362-370.

Banyai, K., Gentsch, J. R., Glass, R. I., Uj, M., Mihaly, I., Szucs, G., 2004. Eight-year survey of human rotavirus strains demonstrates circulation of unusual G and P types in Hungary. J Clin Microbiol. 42, 393-397.

Gouvea, V., Lima, R. C., Linhares, R. E., Clark, H. F., Nosawa, C. M., Santos, N., 1999. Identification of two lineages (WA-like and F45-like) within the major rotavirus genotype P[8]. Virus Res. 59, 141-147.

Kerin, T.K., Kane, E.M., Glass, R.I., Gentsch, J.R., 2007. Characterization of VP6 genesfrom rotavirus strains collected in the United States from 1996–2002. VirusGenes 35, 489–495.

Mijatovic-Rustempasic, S., Banyai, K., Esona, M. D., Foytich, K., Bowen, M. D., Gentsch, J. R., 2011. Genome sequence based molecular epidemiology of unusual US Rotavirus A G9 strains isolated from Omaha, USA between 1997 and 2000. Infect Genet Evol, 11, 522-527.