Supplemental Tables 1 (S1) The list of antibodies for proteins expressing the selected potential target genes.

Symbol / Primary antiboy / Secondery antibody
FOS / c-Fos Rabbit mAb (Cell Signaling Technology) / goat anti-rabbit IgG-HRP(santa cruz)
p27kip1 / Rabbit polyclonal anti-p27 (Santa cruz) / goat anti-rabbit IgG-HRP(santa cruz)
MAP3K10 / Rabbit polyclonal anti-MLK2 (Abcam) / goat anti-rabbit IgG-HRP(santa cruz)
MAPK10 / JNK3 (55A8) Rabbit mAb (Cell Signaling Technology) / goat anti-rabbit IgG-HRP(santa cruz)
MEIS1 / Goat polyclonal anti-MEIS1 (Abcam) / rabbit anti-goat IgG-HRP (santa cruz)
NLK / Rabbit polyclonal anti-NLK (Abcam) / goat anti-rabbit IgG-HRP(santa cruz)
RAB1A / Rabbit polyclonal anti-Rab1A (Abcam) / goat anti-rabbit IgG-HRP(santa cruz)
YWHAG / 14-3-3 γ (D15B7) Rabbit mAb (Cell Signaling Technology) / goat anti-rabbit IgG-HRP(santa cruz)
ZFHX1B / Mouse monoclonal anti-ZFHX1B (Sigma) / goat anti-mouse IgG-HRP (Invitrogen)

Supplemental Tables 2 (S2) Primer sequences for p27kip1 3′UTR reporter construct generation

Construct / Primer sequences
pMIR-p27 kip1-3′UTR-WT / F1: 5′-GGCGGAGCTCATTAAGAATATGTTTCCTTGTTTATCAGATAC-3′
R1: 5′-CGCGGTCGACTATGGAAGTTTTCTTTATTGATTACTTAATGTG-3′
pMIR-p27 kip1-3’UTR-Mu1 / F1: 5′-GGCGGAGCTCATTAAGAATATGTTTCCTTGTTTATCAGATAC-3′
R1: 5′-CGCGGTCGACTATGGAAGTTTTCTTTATTGATTACTTAATGTG-3′
F2: 5′- TAAATGATCTGCCTCTAAAAGCGTTGATTATGCAATTAGGTTTTT
CCTTATTTGCTTCATTGTACT-3′
R2: 5′-AGTACAATGAAGCAAATAAGGAAAAACCTAATTGCATAATCAA
CGCTTTTAGAGGCAGATCATTTA-3′
pMIR-p27 kip1-3′UTR-Mu2 / F1: 5′-GGCGGAGCTCATTAAGAATATGTTTCCTTGTTTATCAGATAC-3′
R1: 5′-CGCGGTCGACTATGGAAGTTTTCTTTATTGATTACTTAATGTG-3′
F3:5′-TCATTGTACTACCTGTGTATATAGTTTTTACCTTTACATAAACTTTGGGGAAGGGAG-3′
R3: 5′-CTCCCTTCCCCAAAGTTTATGTAAAGGTAAAAACTATATACACA
GGTAGTACAATGA-3′
pMIR-p27 kip1-3′UTR-Mu3 / F1: 5′-GGCGGAGCTCATTAAGAATATGTTTCCTTGTTTATCAGATAC-3′
R1: 5′-CGCGGTCGACTATGGAAGTTTTCTTTATTGATTACTTAATGTG-3′
R2: 5′-AGTACAATGAAGCAAATAAGGAAAAACCTAATTGCATAATCAA
CGCTTTTAGAGGCAGATCATTTA-3′
F3: 5′-TCATTGTACTACCTGTGTATATAGTTTTTACCTTTACATAAACTT TGGGGAAGGGAG-3′

* the underlined sequences are restriction enzyme sites.

Supplemental Tables 3 (S3). The potential targets of miR-221/222 predicted by miRGen.

Symbol / PicTar (4way) / PicTar ( 5way) / TargetScanS / miRBase / microRNA.org
FOS / YES / YES / YES / YES / YES
DMRT3 / YES / YES / YES / YES / YES
MAPK10 / YES / YES / YES / YES / YES
NTF3 / YES / YES / YES / YES / YES
p27kip1 / YES / YES / YES / YES / YES
RAB1A / YES / YES / YES / YES / YES
VGLL4 / YES / YES / YES / YES / YES
ZFPM2 / YES / YES / YES / YES
YWHAG / YES / YES / YES / YES
PBX3 / YES / YES / YES / YES
ZFHX1B / YES / YES / YES / YES
NLK / YES / YES / YES / YES
KHDRBS2 / YES / YES / YES / YES
RBM24 / YES / YES / YES / YES
NAP1L5 / YES / YES / YES / YES
TIMP3 / YES / YES / YES / YES
CD4 / YES / YES / YES / YES
ATP1A1 / YES / YES / YES / YES
C1QDC1 / YES / YES / YES / YES
GNAI2 / YES / YES / YES / YES
MEIS1 / YES / YES / YES / YES
HRB / YES / YES / YES / YES
MESDC1 / YES / YES / YES / YES
MAP3K10 / YES / YES / YES / YES
IRX5 / YES / YES / YES
VAPB / YES / YES / YES
ETS1 / YES / YES / YES
ETS2 / YES / YES / YES
ABCC5 / YES / YES / YES
MAT2A / YES / YES / YES
MYLIP / YES / YES / YES
TRPS1 / YES / YES / YES
FNDC3A / YES / YES / YES
UNC84B / YES / YES / YES
SLC25A37 / YES / YES / YES
HOXC10 / YES / YES / YES
C10ORF86 / YES / YES / YES
KIAA1598 / YES / YES / YES
C20ORF23 / YES / YES / YES
PPARGC1A / YES / YES / YES

Supplemental Tables 3 (S3) (continued)

Symbol / PicTar (4way) / PicTar ( 5way) / TargetScanS / miRBase / microRNA.org
PDCD10 / YES / YES / YES
RSBN1L / YES / YES / YES
CCDC64 / YES / YES / YES
CDKN1C / YES / YES
ESR1 / YES / YES
ATXN1 / YES / YES
PDHB / YES / YES
BMF / YES / YES
EBF3 / YES / YES
PAIP2 / YES / YES
DHX15 / YES / YES
FAT2 / YES / YES
CYR61 / YES / YES
CBFB / YES / YES
GNB3 / YES / YES
HOXB5 / YES / YES
ITGB8 / YES / YES
ADAM11 / YES / YES
PAK1 / YES / YES
PPP6C / YES / YES
SAFB / YES / YES
SDC2 / YES / YES
SFRS2 / YES / YES
SMARCA1 / YES / YES
SNAP25 / YES / YES
SNCB / YES / YES
STAC / YES / YES
TCF12 / YES / YES
SMARCA5 / YES / YES
EIF3S1 / YES / YES
SLC4A4 / YES / YES
BNIP3L / YES / YES
FLOT2 / YES / YES
PGGT1B / YES / YES
PHF2 / YES / YES
RALA / YES / YES
GABBR2 / YES / YES
KNS2 / YES / YES
RBMS1 / YES / YES

Supplemental Tables 3 (S3) (continued)

Symbol / PicTar (4way) / PicTar ( 5way) / TargetScanS / miRBase / microRNA.org
SYT1 / YES / YES
MLLT6 / YES / YES
ARID1A / YES / YES
STK4 / YES / YES
PCGF3 / YES / YES
UBE2E3 / YES / YES
GNAI3 / YES / YES
IGF2BP2 / YES / YES
CTCF / YES / YES
MY010 / YES / YES
PDZRN4 / YES / YES
BBC3 / YES / YES
RIMS3 / YES / YES
VASH1 / YES / YES
TMCC1 / YES / YES
CDC2L6 / YES / YES
POGZ / YES / YES
WDR40A / YES / YES
KIAA1267 / YES / YES
ZNF385 / YES / YES
RAP1B / YES / YES
NBEA / YES / YES
MRPS7 / YES / YES
UBE2J1 / YES / YES
PAIP2 / YES / YES
RBMS1 / YES / YES
CHD7 / YES / YES
WDR6 / YES / YES
PCDHA1 / YES / YES
EIF5A2 / YES / YES
TEAD1 / YES / YES
CBFB / YES / YES
C1QDC1 / YES / YES
PHF1 / YES / YES
PHF2 / YES / YES
CPEB4 / YES / YES
ANGPTL2 / YES / YES

Supplemental Tables 4 (S4) GO classification of the predicted targets of miR-221/222.

Function / Genes
Apoptosis / NTF3 / PDCD10 / BMF / SNCB
BNIP3L / STK4 / BBC3 / MAP3K10
mRNA processing / SFRS2 / EIF3S1 / CPEB4 / ANGPTL2
POGZ / RBMS1 / C1QDC1 / IGF2BP2
Cell differentiation / DMRT3 / RBM24 / GNAI2 / PPARGC1A
UNC84B / CYR61 / HOXB5 / TCF12
KNS2 / RAP1B
Cell cycle / p27kip1 / CDKN1C / PDHB / DHX15
FLOT2 / PGGT1B / RALA / GNAI3
VASH1 / WDR6
Transport / RAB1A / ABCC5 / SLC25A37 / C20ORF23
CCDC64 / GNB3 / SNAP25 / STAC
SLC4A4 / RIMS3
Protein kinase activity / MAPK10 / NLK / CD4 / KIAA1598
ADAM11 / PAK1 / PPP6C / UBE2E3
PDZRN4 / CDC2L6 / KIAA1267 / NBEA
UBE2J1 / PAIP2
Transcription / DMRT3 / ZFPM2 / VGLL4 / ZFHX1B
KHDRBS2 / C1QDC1 / MEIS1 / ETS2
TRPS1 / HOXC10 / ESR1 / EBF3
FAT2 / CBFB / SMARCA1 / SMARCA5
PHF2 / MLLT6 / ARID1A / PCGF3
CTCF / ZNF385 / TEAD1 / CBFB
PHF1 / PHF2
Other / IRX5 / PBX3 / YWHAG / MESDC1
NAP1L5 / ATP1A1 / HRB / VAPB
MAT2A / C10ORF86 / RSBN1L / PAIP2
ITGB8 / RBMS1 / SAFB / GABBR2
SYT1 / TMCC1 / WDR40A / MRPS7
CHD7 / PCDHA1 / FOS / ETS1
TIMP3 / MYLIP / FNDC3A / ATXN1
SDC2 / EIF5A2