Supplemental Table S1. The primers used in the study.

A, Primers for amplifying genes cloned into the activation-domain vector (pGADT7) or the DNA binding–domain vector (pGBKT7) of the YTHS.

Primerdesignedbased on
Construct / Primer a / Sequence (5´  3´) / AmplifiedGene / Accession no.
FLHCpro / FLHCpro-Fwd (BamHI) / acgggatccgttattcaacaggggatgttttc / FL HCpro of PVAB11 / AJ296311
FLHCpro-Rev (SalI) / atatgtcgacctatccaaccctgtagtgcttc / AJ296311
HCproΔ325-458 / FLHCpro-Fwd (BamHI) / acgggatccgttattcaacaggggatgttttc / FL HCpro of PVAB11 / AJ296311
HCproΔ1-Rev (SalI) / atatgtcgacctattttggatcacctgagttacc / AJ296311
HCproΔ1-229 / HCproΔ4-Fwd (BamHI) / acgggatccgtggaggtggatacaagcag / FL HCpro of PVAB11 / AJ296311
FLHCpro-Rev (SalI) / atatgtcgacctatccaaccctgtagtgcttc / AJ296311
HCproΔ1-108 / HCproΔ6-Fwd (BamHI) / acgggatccgtttaaatcccaatgttaacgc / FL HCpro of PVAB11 / AJ296311
FLHCpro-Rev (SalI) / atatgtcgacctatccaaccctgtagtgcttc / AJ296311
HCproΔ1-108; 325-458 / HCproΔ6-Fwd (BamHI) / acgggatccgtttaaatcccaatgttaacgc / FL HCpro of PVAB11 / AJ296311
HCproΔ1-Rev (SalI) / atatgtcgacctattttggatcacctgagttacc / AJ296311
HCproΔ1-229; 325-458 / HCproΔ4-Fwd (BamHI) / acgggatccgtggaggtggatacaagcag / FL HCpro of PVAB11 / AJ296311
HCproΔ1-Rev (SalI) / atatgtcgacctattttggatcacctgagttacc / AJ296311
HCproΔ169-458 / FLHCpro-Fwd (BamHI) / acgggatccgttattcaacaggggatgttttc / FL HCpro of PVAB11 / AJ296311
FRAMESHIFT resulted in STOP codon
HCproΔ245-354 / HCproΔ15-Fwd * / ttggatgatggcactcc / FL HCpro of PVAB11 / AJ296311
HCproΔ15-Rev * / tccatttggaactcttctctc / AJ296311
TeIF4E / T4E-YTHS-Fwd (EcoRI) / ccggaattcatggttgatgaagtagag / eIF4Ea,b of tobacco / AY702653
T4E-YTHS-Rev (SalI) / cggtcgacctacacagaataacgattc / AY702653
TeiIF(iso)4E / Tiso4E-YTHS-Fwd (Bam HI) / ccgggatccttgcagcagctgaaatggagag / eIF(iso)4Ea,b of tobacco / dbEST Id: 10346837
Tiso4E-YTHS-Rev (XhoI) / ccgctcgagctcgactcacacagtatatc / dbEST Id: 10346837
PeIF4E / P4E-YTHS-Fwd (BamHI) / ccgggatccttgcagcagctgaaatggagag / eIF4Ea,b of potato / dbEST Id:8322240
P4E-YTHS-Rev (SalI) / cgacgtcgacctatacggtgtaacgattcttg / dbEST Id:8994232
PeIF(iso)4E / Piso4E-YTHS-Fwd (BamHI) / ccgggatccttgccaccgaagcaccggtagag / eIF(iso)4Ea,b of potato / dbEST Id: 10346837
Piso4E-YTHS-Rev (SalI) / cgacgtcgactcacacagtatatcggctcttag / dbEST Id: 10346837
a The restriction site for the enzyme mentioned in inparantheses was added to the primer sequence and is underlined.
* No restrictionsiteadded.

B, Primers used for amplification of genes cloned into the pRT expression cassette and subsequently transferred to a binary vector to be used for transformation of A. tumefaciens.

Primerdesignedbased on
Construct / Primer a / Sequence (5´  3´) / AmplifiedGene / Accession no.
pRT_YN-PVAHCpro / PVA-HCpro-Fwd (NcoI) / gcgccatggcgtattcaacaggggatgttttc / HCpro of PVA / AJ296311
PVA-HCpro-Rev (NcoI) / gcgccatggctatccaaccctgtagtgcttc / AJ296311
pRT_YN-TEVHCpro / TEV-HCpro-Fwd (NcoI) / ggcccatggggagcgacaaatcaatctctga / HCpro of TEV / M15239.1
TEV-HCpro-Rev (NcoI) / ggcccatggctatccaacattgtaagttttc / M15239.1
pRT_YN-PVYHCpro / PVY-N-HCpro-Fwd (NcoI) / ggcccatggggtcaagcgctgaaagcttttg / HCpro of PVY / X97895
PVY-N-HCpro-Rev (NcoI) / ggcccatggctaaccaactctatagtgcttaatg / X97895
pRT_T4E-YC / T4E-Fwd (XhoI) / aagctcgagatggttgatgaagtagagaaaccg / eIF4Ea,b of tobacco / AY702653
T4E-Rev (XhoI) / atgctcgagcacagaataacgattcttggc / AY702653
pRT_YC-T4E / T4E-Fwd (NcoI) / acgccatggttgatgaagtagagaaaccg / eIF4Ea,b of tobacco / AY702653
T4E-Rev (NcoI) / acgccatggctacacagaataacgattcttg / AY702653
pRT_Tiso4E-YC / Tiso4E-Fwd (XhoI) / ccgctcgagatggccaccgaagcaccgg / eIF(iso)4Ea,b of tobacco / dbEST Id: 10346837
Tiso4E-Rev (XhoI) / ccgctcgagctcgactcacacagtatatc / dbEST Id: 10346837
pRT_YC-Tiso4E / Toiso4E-Fwd (NcoI) / ccgccatggccaccgaagcaccgg / eIF(iso)4Ea,b of tobacco / dbEST Id: 10346837
Tiso4E-Rev (NcoI) / cggccatggctactcgactcacacagtatatc / dbEST Id: 10346837
pRT_P4E-YC / P4E-Fwd (XhoI) / ggcctcgagatgcttgcagcagctgaaatggag / eIF4Ea,b of potato / dbEST Id:8322240
P4E-Rev (XhoI) / ggcctcgagtacggtgtaacgattcttgg / dbEST Id:8994232
pRT_YC-P4E / P4E-Fwd (NcoI) / ggcccatggggcttgcagcagctgaaatggag / eIF4Ea,b of potato / dbEST Id:8322240
P4E-Rev (NcoI) / ggcccatggctatacggtgtaacgattcttgg / dbEST Id:8994232
pRT_Piso4E-YC / Piso4E-Fwd (XhoI) / ccgctcgagatggccaccgaagcaccgg / eIF(iso)4Ea,b of potato / dbEST Id: 10346837
Piso4E-Rev (XhoI) / ccgctcgagtcacacagtatatcggctc / dbEST Id: 10346837
pRT_YC-Piso4E / Piso4E-Fwd (NcoI) / ccgccatggccaccgaagcaccgg / eIF(iso)4Ea,b of potato / dbEST Id: 10346837
Piso4E-Rev (NcoI) / cggccatggctatcacacagtatatcggc / dbEST Id: 10346837
aThe restriction site for the enzyme metioned in parantheses was added to the primer sequence and is underlined.