Supplemental table S1: Accession numbers of 55 protein sequences used for the phylogenetic analysis of full-size ABCG transporters.

Plant species / New systematic name / Synonyms / Accession number / Reference
Arabidopsis thaliana / AtABCG29 / AtPDR1 / NP_566543 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG30 / AtPDR2 / NP_193258 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG31 / AtPDR3 / NP_180555 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG32 / AtPDR4 / NP_180259 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG33 / AtPDR5 / NP_181265 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG34 / AtPDR6 / NP_181179 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG35 / AtPDR7 / NP_172973 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG36 / AtPDR8 / NP_176196 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG37 / AtPDR9 / NP_190916 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG38 / AtPDR10 / Q7PC85 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG39 / AtPDR11 / NP_176867 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG40 / AtPDR12 / NP_173005 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG41 / AtPDR13 / Q7PC83 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG42 / AtPDR14 / Q7PC82 / Verrier et al. [29]
Arabidopsis thaliana / AtABCG43 / AtPDR15 / Q7PC81 / Verrier et al. [29]
Glycine max / GmABCG1 / GmPDR12 / NP_001237697 / Eichhorn et al. [41]
Hordeum vulgare / HvABCG31 / BAK52287 / Chen et al. [50]
Nicotiana
plumbaginifolia / NpABCG1 / NpPDR1 / Q949G3 / Jasinski et al. [38]
Nicotiana
plumbaginifolia / NpABCG2 / NpPDR2 / Q2PCF1 / Bienert et al. [62]
Nicotiana
plumbaginifolia / NpABCG5a / NpPDR5 / AFN42936 / Bienert et al. [62]
Nicotiana tabacum / NtABCG1 / NtPDR1 / BAB92011 / Sasabe et al. [39]
Nicotiana tabacum / NtABCG3 / NtPDR3 / Q5W274 / Ducos et al. [45]
Nicotiana tabacum / NtABCG5 / NtPDR5 / AFN42937 / Bienert et al. [62]
Oriza sativa / OsABCG45 / OsPDR1 / LOC_Os08g43120.1 / Verrier et al. [29]
Oriza sativa / OsABCG41 / OsPDR2 / LOC_Os02g32690.1 / Verrier et al. [29]
Oriza sativa / OsABCG48 / OsPDR3 / LOC_Os11g37700.1 / Verrier et al. [29]
Oriza sativa / OsABCG40 / OsPDR4 / LOC_Os02g21340.1 / Verrier et al. [29]
Oriza sativa / OsABCG43 / OsPDR5 / LOC_Os07g33780.1 / Verrier et al. [29]
Oriza sativa / OsABCG31 / OsPDR6 / LOC_Os01g08260.1 / Verrier et al. [29]
Oriza sativa / OsABCG39 / OsPDR7 / LOC_Os02g11760.1 / Verrier et al. [29]
Oriza sativa / OsABCG37 / OsPDR8 / LOC_Os01g42410.1 / Verrier et al. [29]
Oriza sativa / OsABCG36 / OsPDR9 / LOC_Os01g42380.1 / Verrier et al. [29]
Oriza sativa / OsABCG34 / OsPDR10 / LOC_Os01g42350.1 / Verrier et al. [29]
Oriza sativa / OsABCG35 / OsPDR11 / LOC_Os01g42370.1 / Verrier et al. [29]
Oriza sativa / OsABCG42 / OsPDR12 / LOC_Os06g36090.2 / Verrier et al. [29]
Oriza sativa / OsABCG38 / OsPDR15 / LOC_Os01g52560.1 / Verrier et al. [29]
Oriza sativa / OsABCG32 / OsPDR16 / LOC_Os01g24010.1 / Verrier et al. [29]
Oriza sativa / OsABCG44 / OsPDR17 / LOC_Os08g29570.1 / Verrier et al. [29]
Oriza sativa / OsABCG47 / OsPDR19 / LOC_Os09g16380.1 / Verrier et al. [29]
Oriza sativa / OsPDR20 / LOC_Os09g16330.2 / Verrier et al. [29]
Oriza sativa / OsABCG46 / OsPDR21 / LOC_Os09g16290.1 / Verrier et al. [29]
Oriza sativa / OsABCG49 / OsPDR22 / LOC_Os12g13720.1 / Verrier et al. [29]
Oriza sativa / OsABCG50 / OsPDR23 / LOC_Os12g32820.1 / Verrier et al. [29]
Solanum tuberosum / StABCG1 / AEO22187 / Ruocco et al. [33]
Solanum tuberosum / StABCG2 / AEB65936 / Ruocco et al. [33]
Solanum tuberosum / StABCG3 / AEO22188 / Ruocco et al. [33]
Solanum tuberosum / StABCG4 / AEO22189 / Ruocco et al. [33]
Spirodela polyrrhiza / SpABCG1 / SpTUR2 / O24367 / Smart et al. [44]
Triticum aestivum / TaPDR1 / ACL80650 / Shang et al. [49]
Triticum aestivum / TaLr34 / ACN41354 / Krattinger et al. [48]
Triticum aestivum / TaABCG2 / AEY84029 / This work
Triticum aestivum / TaABCG3 / AEY84030 / This work
Triticum aestivum / TaABCG4 / AEY84031 / This work
Triticum aestivum / TaABCG5 / AEY84032 / This work
Triticum aestivum / TaABCG6 / AEY84033 / This work

Supplemental figure S1: DNA sequence alignment of the 432 bp C terminal region spanned between Walker A and PDR signature 3 conserved motifs of wheat (T. aestivum) TaPDR1 (FJ185035), TaABCG2 (JN799999), TaABCG3 (JN800000), TaABCG4 (JN800001), TaABCG5 (JN800002), TaABCG6 (JN800003) full-size transporters isolated from F. graminearum resistant genotype Centenaire. The sequences were aligned using Clustal W.

Supplemental figure S2: Determination of the chromosome location of TaABCG2 gene using gene specific primers and set of 78 deletion lines derived from Chinese Spring wheat. TaABCG2 was determined on chromosome wheat chromosome 5BL in chromosomal bin 5BL1-0.55-1.00. The asterisks show the corresponding deletion line where TaABCG2 was assigned. M: DNA size marker.

Supplemental figure S3: Southern blot analysis of EcoRI-digested genomic DNA from 21 nullisomic-tetrasomic Chinese Spring lines with TaABCG3, TaABCG5 and TaABCG6 cDNA as probes. The chromosomal localization of TaABCG3 was determined to be on chromosome 7A, B and D. TaABCG5 was positioned on chromosomes 3A, B, and D and TaABCG6 was situated on 2B, 5A, and 5D. The asterisks show where the ABCG genes were located.