Supplemental table 2. Sequence comparison of human and porcine collagen Type Iα2 chain.

Number / Amino acid sequence / Number
human
porcine
human
porcine / 1
1
31
31 / MLSFVDTRTL LLLAVTLCLA TCQSLQEETV
MLSFVDTRTL LLLAVTSCLA TCQSLQEATA
RKGPAGDRGP RGERGPPGPP GRDGEDGPTG
RKGPTGDRGP RGERGPPGPP GRDGDDGI PG / 30
30
60
60
human
porcine
human
porcine / 61
61
91
91 / PPGPPGPPGP PGLGGNFAAQ YDGKGVGLGP
PPGPPGPPGP PGLGGNFAAQ YDGKGVGAGP
GPMGLMGPRG PPGAAGAPGP QGFQGPAGEP
GPMGLMGPRG PPGAVGAPGP QGFQGPAGEP / 90
90
120
120
human
porcine
human
porcine / 121
121
151
151 / GEPGQTGPAG ARGPAGPPGK AGEDGHPGKP
GEPGQTGPAG ARGPPGPPGK AGEDGHPGKP
GRPGERGVVG PQGARGFPGT PGLPGFKGIR
GRPGERGVVG PQGARGFPGT PGLPGFKGIR / 150
150
180
180
human
porcine
human
porcine / 181
181
211
211 / GHNGLDGLKG QPGAPGVKGE PGAPGENGTP
GHNGLDGLKG QPGAPGVKGE PGAPGENGTP
GQTGARGLPGERGRVGAPGPAGARGSDGSV
GQTGARGLPGERGRVGAPGPAGARGNDGSV / 210
210
240
240
human
porcine
human
porcine / 241
241
271
271 / GPVGPAGPIG SAGPPGFPGAPGPKGEI GAV
GPVDPAGPIG SAGPPGFPGAPGPKGELGPV
a
GNAGPAGPAGPRGEVGLPGLSGPVGPPGNP
GNPGPAGPAGPRGEVGLPGVSGPVGPPGNP / 270
270
300
300
human
porcine
human
porcine / 301
301
331
331 / GANGLTGAKGAAGLPGVAGAPGLPGPRGIP
GANGLPGAKGAAGLPGVAGAPGLPGPRGIP
GPVGAAGATGARGLVGEPGPAGSKGESGNK
GPAGAAGATGARGLVGEPGPAGSKGESGNK / 330
330
360
360
human
porcine
human
porcine / 361
361
391
391 / GEPGSAGPQGPPGPSGEEGKRGPNGEAGSA
GEPGAAGPQGPPGPSGEEGKRGPNGEVGSA
GPPGPPGLRGSPGSRGLPGADGRAGVMGPP
GPPGPPGLRGNPGSRGLPGADGRAGVMGPP / 390
390
420
420
human
porcine
human
porcine / 421
421
451
451 / GSRGASGPAGVRGPNGDAGRPGEPGLMGPR
GSRGP TGPAGVRGPNGDSGR PGEPGLMGPR
b1
GLPGSPGNI GPAGKEGPVGLPGIDGRPGPI
GFPGSPGNVGPAGKEGPAGLPGIDGRPGPI / 450
450
480
480
human
porcine
human
porcine / 481
481
511
511 / GPAGARGEPGNIGFPGPKGPTGDPGKNGDK
GPAGARGEPGNIGFPGPKGPTGDPGKNGEK
GHAGLAGARGAPGPDGNNGAQGPPGPQGVQ
GHAGLAGARGAPGPDGNNGAQGPPGPQGVQ / 510
510
540
540
human
porcine
human
porcine / 541
541
571
571 / GGKGEQGPPGPPGFQGLPGP SGPAGEVGKP
GGKGEQGPAGPPGFQGLPGPAGTAGEVGKP
GERGLHGEFG LPGPAGPRGERGPPGESGAA
GERGI P GEFG LPGPAGPRGERGPPGESGAA
b2 / 570
570
600
600
human
porcine
human
porcine / 601
601
631
631 / GPTGPIGSRGPSGPPGPDGNKGEPGVVGAV
GPAGPIGSRGPSGPPGPDGNKGEPGVLGAP
GTAGPSGPSGLPGERGAAGIPGGKGEKGEP
GTAGPSGPSGLPGERGAAGIPGGKGEKGET
/ 630
630
660
660
human
porcine
human
porcine / 661
661
691
691 / GLRGE I GNPGRDGARGAPGAVGAPGPAGAT
GLRGDVGSPGRDGARGAPGAVGAPGPAGAN
b3
GDRGEAGAAGPAGPAGPRGSPGERGEVGPA
GDRGEAGPAGPAGPAGPRGSPGERGEVGPA / 690
690
720
720
human
porcine
human
porcine / 721
721
751
751 / GPNGFAGPAGAAGQPGAKGERGAKGPKGEN
GPNGFAGPAGAAGQPGAKGERGTKGPKGEN
GVVGPTGPVGAAGPAGPNGPPGPAGSRGDG
GPVGPTGPVGAAGPAGPNGPPGPAGSRGDG / 750
750
780
780
human
porcine
human
porcine / 781
781
811
811 / GPPGMTGFPGAAGRTGPPGPSGISGPPGPP
GPPGATGFPGAAGR I GPPGPSGISGPPGPP
GPAGKEGLRGPRGDQGPVGRTGEVGAVGPP
GPAGKEGLRGPRGDQGPVGRTGET GAS GPP / 810
810
840
840
human
porcine
human
porcine / 841
841
871
871 / GFAGEKGPSGEAGTAGPPGTPGPQGLLGAP
GFAGEKGPSGEP GTAGPPGTPGPQGI LGAP
GILGLPGSRGERGLPGVAGAVGEPGPLGIA
GFLGLPGSRGERGLPGVAGSVGEPGPLGIA / 870
870
900
900
human
porcine
human
porcine / 901
901
931
931 / GPPGARGPPGAVGSPGVNGAPGEAGRDGNP
GPPGARGPPGAVGNPGVNGAPGEAGRDGNP
GNDGPPGRDGQPGHKGERGYPGNI GPVGAA
GSDGPPGRDGQAGHKGERGYPGNPGPAGAA / 930
930
960
960
human
porcine
human
porcine / 961
961
991
991 / GAPGPHGPVGPAGKHGNRGE TGPSGPVGPA
GAPGPQGAVGPAGKHGNRGEPGPAGSVGPA
GAVGPRGPSGPQGIRGDKGEPGEKGPRGLP
GAVGPRGPSGPQGIRGEKGEPGDKGPRGLP / 990
990
1020
1020
human
porcine
human
porcine / 1021
1021
1051
1051 / GLKGHNGLQGLPGI AGHHGDQGAPGSVGPA
GLKGHNGLQGLPGLAGHHGDQGAPGPVGPA
GPRGPAGPSGPAGKDGRTGHPGTVGPAGIR
GPRGPAGPSGPAGKDGRTGQPGAVGPAGIR / 1050
1050
1080
1080
human
porcine
human
porcine / 1081
1081
1111
1111 / GPQGHQGPAGPPGPPGPPGPPGVSGGGYDF
GSQGS QGPAGPPGPPGPPGPPGPSGGGYDF
GYDGDFYRADQPRSAPSLRPKDYEVDATLK
GYEGDFYRADQPRSP PSLRPKDYEVDATLK / 1110
1110
1140
1140
human
porcine
human
porcine / 1141
1141
1171
1171 / SLNNQIETLLTPEGSRKNPARTCRDLRLSH
SLNNQIETLLTPEGSRKNPARTCRDLRLSH
PEWSSGYYWIDPNQGCTMDAIKVYCDFSTG
PEWSSGYYWIDPNQGCTMDAIKVYCDFSTG / 1170
1170
1200
1200
human
porcine
human
porcine / 1201
1201
1231
1231 / ETCIRAQPENIPAKNWYRSSKDKKHVWLGE
ETCIRAQPENIPAKNWYRNSKVKKHVWLGE
TINAGSQFEYNV EGVTSKEMATQLAFMRLL
TINGGTQFEYNMEGVTTKEMATQLAFMRLL / 1230
1230
1260
1260
human
porcine
human
porcine / 1261
1261
1291
1291 / ANYASQNITYHCKNSIAYMDEETGNLKKAV
ANHASQNITYHCKNSIAYMDEETGNLKKAV
ILQGSNDVELVAEGNSRFTYTVLVDGCSKK
ILQGSNDVELVAEGNSRFTYTVLVDGCSKK / 1290
1290
1320
1320
human
porcine
human
porcine / 1321
1321
1251
1251 / TNEWGKTIIEYKTNKPSRLPFLDIAPLDIG
TNEWRKTIIEYKTNKPSRLPI LDIAPLDIG
GADQEF FVDI GPVCFK
DADQEVSVDVGPVCFK / 1350
1250
1366
1366

BLAST search result: sequence identity 1280/1366 (94%), positives hits1311/1366 (95%). Signal sequence, N-Propeptide and N-Propeptideare absent in mature collagen and the difference locus in these region have no role in immunogenicity or antigenicity. Mature collagen. Difference locus. Similar locus

a: At position 244 of the human peptide, glycine (Gly-Pro-Ala) leads to helix formation. In the porcine peptide, there is an aspartic acid residue at this position (Asp-Pro-Ala), which precludes a helix structure, that is likely to be a antigenic determinant.

b1, b2 and b3:It will be easier to recognize a single Gly-X-Y triplet at which both X and Y residues diverge between human and porcine