Supplemental Table 1. Characteristics of the selected studies using the RBM Human DiscoveryMAP in comparison to the ADNI dataset

Source / ADNI / Hu et al. 201011 / Öhrfelt et al. 201112 / Craig-Schapiro
et al. 201113 / Britschgiet al. 201114
Cohort / Multicenter / PENN / Gothenburg / OPTIMA / WU-ADRC / Multicenter
N* / 92 / 69 / 33 / 66 / 52 / 24 / 48 / 63 / 242 / 91 / 25 / 18
Age, years* / 76 / 75 / 72 / 71 / 67 / 65 / 69 / 76 / 72 / 75 / 76 / 72
Education, years* / 16 / 15 / 16 / 15 / - / - / - / 16/ 16
Sex, %men* / 50 / 56 / 42 / 47 / 60 / 75 / 46 / 63 / 65 / 51 / 72 / 64
MMSE, points* / 29 / 24 / 30 / 18 / 30 / 26 / 29 / 12 / 29 / 25 / 29 / 20
ApoE ε4, %carrier* / 24 / 71 / - / - / - / 32 / 55 / 33 / 76

PubMed/MEDLINE was searched from the year 2009 on using the terms: Alzheimer; CSF; multiple biomarkers. Only studies using the RBM Human DiscoveryMAP were included. Exclusion criteria were: non case-control studies; single biomarker studies; studies using other techniques.

* controls / patients; PENN: The University of Pennsylvania Alzheimer’s Disease Clinical Core; Gothenburg; Gothenburg MCI study; OPTIMA: The Oxford Project to Investigate Memory and Aging; WU-ADRC: The Knight Alzheimer Disease Research Center at Washington University.

Supplemental Table 2.Individual maximum follow-up times of patients with mild cognitive impairment

Follow-up time (months) / N / Percent
12 / 5 / 3.4
18 / 8 / 5.4
24 / 35 / 23.5
36 / 78 / 52.3
48 / 16 / 10.7
60 / 7 / 4.7

Supplemental Table 3. RBM biomarkers

AXL / AXL Receptor Tyrosine Kinase
ADI / Adiponectin
ADIPOQ / Agouti-Related Protein
AAT / Alpha-1-Antitrypsin
A1Micro / Alpha-1-Microglobulin
A2Macro / Alpha-2-Macroglobulin
AFP / Alpha-Fetoprotein
AR / Amphiregulin
ANG2 / Angiopoietin-2
ACE / Angiotensin-Converting Enzyme
ApoAI / Apolipoprotein A-I
ApoCIII / Apolipoprotein C-III
ApoD / Apolipoprotein D
ApoE / Apolipoprotein E
ApoH / Apolipoprotein H
LPA / Apolipoprotein
BLC / B Lymphocyte Chemoattractant
B2M / Beta-2-Microglobulin
BTC / Betacellulin
BMP6 / Bone Morphogenetic Protein 6
BDNF / Brain-Derived Neurotrophic Factor
CRP / C-Reactive Protein
CD40 / CD 40 antigen
CD40L / CD40 Ligand
Calcitonin / Calcitonin
CA125 / Cancer Antigen 125
CA199 / Cancer Antigen 19-9
CEA / Carcinoembryonic Antigen
HCC4 / Chemokine CC-4
CgA / Chromogranin-A
CNTF / Ciliary Neurotrophic Factor
CLU / Clusterin
C3 / Complement C3
Cortisol / Cortisol
CKMB / Creatine Kinase-MB
CystatinC / Cystatin-C
E-Selectin / E-Selectin
en-RAGE / EN-RAGE
ET1 / Endothelin-1
Eotaxin1 / Eotaxin-1
Eotaxin3 / Eotaxin-3
EGF / Epidermal Growth Factor
EPR / Epiregulin
ENA / Epithelial-Derived Neutrophil-Activating
EPO / Erythropoietin
FAS / FASLG Receptor
FactorVII / Factor VII
FasL / Fas Ligand
FABP / Fatty Acid-Binding Protein, heart
FRTN / Ferritin
Fibrinogen / Fibrinogen
FGF4 / Fibroblast Growth Factor 4
FGFb / Fibroblast Growth Factor basic
FSH / Follicle-Stimulating Hormone
GLP1 / Glucagon-like Peptide 1, total
GCSF / Granulocyte Colony-Stimulating Factor
GMCS / Granulocyte-Macrophage Colony-Stimulatin
GH / Growth Hormone
GROa / Growth-Regulated alpha protein
Haptoglobin / Haptoglobin
HBEGF / Heparin-Binding EGF-Like Growth Factor
HGF / Hepatocyte Growth Factor
IgA / Immunoglobulin A
IgE / Immunoglobulin E
IGM / Immunoglobulin M
Insulin / Insulin
IGF / Insulin-like Growth Factor-Binding Prote
ICAM1 / Intercellular Adhesion Molecule 1
IFNg / Interferon gamma
IFI10 / Interferon gamma Induced Protein 10
IL1a / Interleukin-1 alpha
IL1b / Interleukin-1 beta
IL1r / Interleukin-1 receptor antagonist
IL10 / Interleukin-10
IL12p40 / Interleukin-12 Subunit p40
IL12p70 / Interleukin-12 Subunit p70
IL13 / Interleukin-13
IL15 / Interleukin-15
IL16 / Interleukin-16
IL17 / Interleukin-17
IL18 / Interleukin-18
IL2 / Interleukin-2
IL23 / Interleukin-23
IL25 / Interleukin-25
IL3 / Interleukin-3
IL4 / Interleukin-4
IL5 / Interleukin-5
IL6 / Interleukin-6
IL6r / Interleukin-6 Receptor
IL7 / Interleukin-7
IL8 / Interleukin-8
LOX / Lectin-Like Oxidized LDL Receptor 1
Leptin / Leptin
LH / Luteinizing Hormone
Lymphotactin / Lymphotactin
MCSF1 / Macrophage Colony-Stimulating Factor 1
MIP1alpha / Macrophage Inflammatory Protein-1 alpha
MIP1beta / Macrophage Inflammatory Protein-1 beta
MIP3alpha / Macrophage Inflammatory Protein-3 alpha
MMIF / Macrophage Migration Inhibitory Factor
MDC / Macrophage-Derived Chemokine
MMLDL / Malondialdehyde-Modified Low-Density Lip
MMP2 / Matrix Metalloproteinase-2
MMP3 / Matrix Metalloproteinase-3
MMP9 / Matrix Metalloproteinase-9
MCP1 / Monocyte Chemotactic Protein 1
MCP2 / Monocyte Chemotactic Protein 2
MCP3 / Monocyte Chemotactic Protein 3
MCP4 / Monocyte Chemotactic Protein 4
MI / Monokine Induced by Gamma Interferon
MPIF / Myeloid Progenitor Inhibitory Factor 1
MPO / Myeloperoxidase
Myoglobin / Myoglobin
NPBN / N-terminal prohormone of brain natriuret
NGFb / Nerve Growth Factor beta
NrCAM / Neuronal Cell Adhesion Molecule
NGAL / Neutrophil Gelatinase-Associated Lipocal
Osteopontin / Osteopontin
PPP / Pancreatic Polypeptide
PLGF / Placenta Growth Factor
PAI1 / Plasminogen Activator Inhibitor 1
PDGF / Platelet-Derived Growth Factor BB
PAPPA / Pregnancy-Associated Plasma Protein A
PGR / Progesterone
PRL / Prolactin
PSAf / Prostate-Specific Antigen, Free
PAP / Prostatic Acid Phosphatase
PARC / Pulmonary and Activation-Regulated Chemo
RAGE / Receptor for advanced glycosylation end
Resistin / Resistin
S100B / S100 calcium-binding protein B
SAP / Serum Amyloid P-Component
SGOT / Serum Glutamic Oxaloacetic Transaminase
SHBG / Sex Hormone-Binding Globulin
Sortilin / Sortilin
SCF / Stem Cell Factor
SOD1 / Superoxide Dismutase 1, Soluble
I3 / T Lymphocyte-Secreted Protein I-309
Tcee / T-Cell-Specific Protein RANTES
TRAILR3 / TNF-Related Apoptosis-Inducing Ligand Receptor 3
THP / Tamm-Horsfall Urinary Glycoprotein
TNC / Tenascin-C
tTEST / Testosterone, Total
TM / Thrombomodulin
THPO / Thrombopoietin
THPO1 / Thrombospondin-1
TSH / Thyroid-Stimulating Hormone
TBG / Thyroxine-Binding Globulin
TF / Tissue Factor
TIM1 / Tissue Inhibitor of Metalloproteinases 1
TGFa / Transforming Growth Factor alpha
TGFb / Transforming Growth Factor beta-3
TFF3 / Trefoil Factor 3
TNFR2 / Tumor Necrosis Factor Receptor 2
TNFa / Tumor Necrosis Factor alpha
TNFb / Tumor Necrosis Factor beta
VCAM1 / Vascular Cell Adhesion Molecule-1
VEGF / Vascular Endothelial Growth Factor
vWF / von Willebrand Factor