Supplemental Table 1. Sarcoma cell lines growth medium and supplements.

Cell Line / Initial Source / Type / Media type
1 / RD-ES / ATCC HTB-166 / Ewing Sarcoma / RPMI 1640 15% FBS 1%Glut
2 / A-204 / NCI Repository / Rhabdoid tumor / RPMI 1640 10% FBS 1%Glut
3 / A-673 / NCI Repository / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
4 / CHA 59 / NCI Repository / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
5 / CHP-100 / MSKCC / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
6 / DDLS / MSKCC / Dedifferentiated liposarcoma / RPMI 1640 10% FBS 1%Glut
7 / ES-1 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
8 / ES-2 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
9 / ES-3 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
10 / ES-4 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
11 / ES-6 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
12 / ES-7 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
13 / ES-8 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
14 / EW8 / PPTP-Houghton / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
15 / HOS / NCI Repository / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
16 / Hs 913.T / NCI Repository / Fibrosarcoma / RPMI 1640 10% FBS 1%Glut
17 / HT-1080 / NCI Repository / Fibrosarcoma / RPMI 1640 10% FBS 1%Glut
18 / Hu09 / MSKCC Repository / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
19 / LS141 / MSKCC / Dedifferentiated liposarcoma / RPMI 1640 10% FBS 1%Glut
20 / MES-SA / NCI Repository / Uterine Sarcoma / RPMI 1640 10% FBS 1%Glut
21 / MES-SA Dx5 / NCI Repository / Uterine Sarcoma / RPMI 1640 10% FBS 1%Glut
22 / MHM-25 / NCI Repository / Sarcoma / RPMI 1640 10% FBS 1%Glut
23 / MHM-8 / NCI Repository / Sarcoma / RPMI 1640 10% FBS 1%Glut
24 / MPNST / MSKCC / Malignant peripheral neural sheath tumor / RPMI 1640 10% FBS 1%Glut
25 / OHS / NCI Repository / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
26 / RD / NCI Repository / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
27 / Rh18 / NCI Repository / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
28 / Rh28 / NCI Repository / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
29 / Rh30 / NCI Repository / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
30 / Rh28 PX11/LPAM / PPTP-Houghton / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
31 / Rh36 / PPTP-Houghton / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
32 / Rh41 / PPTP-Houghton / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
33 / SAOS-2 / NCI Repository / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
34 / SJCRH30(RMS13) / ATCC CRL-2061 / Rhabdomyosarcoma / RPMI 1640 10% FBS 1%Glut
35 / SJSA-1 / ATCC CRL-2098 / Osteosarcoma / RPMI 1640 10% FBS 1%Glut
36 / SK-N-MC / COG- Reynolds / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
37 / SK-UT-1B / MSKCC / Leiomyosarcoma / RPMI 1640 10% FBS 1%Glut
38 / ST8814 / MSKCC / Malignant peripheral neural sheath tumor / RPMI 1640 10% FBS 1%Glut
39 / TC-32 / COG- Reynolds / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
40 / TC-71 / COG- Reynolds / Ewing Sarcoma / RPMI 1640 10% FBS 1%Glut
41 / SK-ES-1 / ATCC HTB-86 / Ewing Sarcoma / McCoys 15%FBS 1%Gut
42 / G-401 / ATCC CRL-1441 / Rhabdoid Tumor / McCoys 10%FBS 1%Gut
43 / U-2 OS / ATCC HTB-96 / Osteosarcoma / McCoys 10%FBS 1%Gut
44 / SW 1353 / ATCC HTB-94 / Chondrosarcoma / Leibovitz L15 10%
45 / SW 684 / ATCC HTB-91 / Fibrosarcoma / Leibovitz L15 10%
46 / SW 982 / ATCC HTB-93 / Atypical Synovial sarcoma / Leibovitz L15 10%
47 / CHLA-10 / COG- Reynolds / Ewing Sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
48 / CHLA-25 / COG- Reynolds / Ewing sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
49 / CHLA-258 / COG- Reynolds / Ewing Sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
50 / CHLA-32 / COG- Reynolds / Ewing sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
51 / CHLA-9 / COG- Reynolds / Ewing Sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
52 / COG-E-352 / COG- Reynolds / Ewing sarcoma / IMDM 20%FBS+ITS(insulin,transferrin,selenous)
53 / KHOS NP / ATCC CRL-1544 / Osteosarcoma / EMEM 10%FBS
54 / KHOS-240S / ATCC CRL-1545 / Osteosarcoma / EMEM 10%FBS
55 / KHOS-312H / ATCC CRL-1546 / Osteosarcoma / EMEM 10%FBS
56 / SK-LMS-1 / ATCC HTB-88 / Leiomyosarcoma / EMEM 10%FBS
57 / SK-UT-1 / ATCC HTB-114 / Leiomyosarcoma / EMEM 10%FBS
58 / ASPS-1 / NCI Repository / Alveolar Soft Part sarcoma / DMEM-F12 50/50 10%FBS
59 / Hs 706.T / ATCC CRL-7447 / Giant Cell Sarcoma / DMEM 10%FBS
60 / Hs 729 / ATCC HTB-153 / Rhabdomyosarcoma / DMEM 10%FBS
61 / HSSY-II / MSKCC / Synovial sarcoma / DMEM 10%FBS
62 / SYO-1 / MSKCC / Synovial sarcoma / DMEM 10%FBS
63 / VA-ES-BJ / ATCC CRL-2138 / Epithelioid Sarcoma / DMEM 10%FBS

Supplemental Table 2. Compounds screened in the sarcoma cell lines.

1