Supplemental Table 1.Primers used for PCR amplification

Gene / GenBank accession
number / Primer name / Froward primer(5’-3’) / Reverse primer(5’-3’) / Use
OsTLP1 / AK103583 / TLP8-RT-F/R / GCTTTCCAGGCATTTGCAA / CAGGCCAGTTTTGTGTCGAA / Real-time PCR
OsTLP2 / AK120706 / TLP12-RT-F/R / GCTCCCGCTTCTCGGAAA / ACCCGGCCACGGAAGTT / Real-time PCR
OsTLP3 / AK104333 / TLP7-RT-F/R / GACACGCAGCCTCCATACAAT / TCGTCCGTTCTTGTGAGCAA / Real-time PCR
OsTLP4 / AK102298 / TLP14-RT-F/R / CAAAGAACCCTTCCAGCACCTA / CGAGTCCCCAAGACATTCAGTT / Real-time PCR
OsTLP5 / AK102221 / TLP3-RT-F/R / AGTGCTCGCTTCTCTGACATCA / CTGTAGCTGCTCATGCCATCTC / Real-time PCR
OsTLP6 / AK070139 / TLP13-RT-F/R / CCAACTCCATCACAGCCTGTT / CCTTCCCGAATTGCAGTATGA / Real-time PCR
OsTLP7 / AK060587 / TLP11-RT-F/R / CGCTATCCTATCTCGGCATTTC / GGTGTCAAAGCTGCTCAGACAT / Real-time PCR
OsTLP8 / AK064855 / TLP5-RT-F/R / TATCACCGCGATTGCCACTA / GCATGGTGCATTGCATTCTC / Real-time PCR
OsTLP9 / AK070401 / TLP1-RT-F/R / GCTAGCTCGAAGGATGAGGAAA / CATCGACCTTACCAAACTGCAA / Real-time PCR
OsTLP10 / AK061747 / TLP9-RT-F/R / CCACCATACGATGGTGCAAA / TGCAAAGCGACGACTCGAT / Real-time PCR
OsTLP11 / EU328286 / TLP10-RT-F/R / ACGGATCTGCCCCCAAATA / TGAGATCTGCCCAACATTGAAG / Real-time PCR
TLP10-cds1-F/R / ACAAGCGGAGGAGGAGTG / CAGCATCAAGGGAAATAACATAC / PCR sequencing
TLP10-cds2-F/R / TCGTCAAGCGAAACAAGAAG / GTAGCAGCCCAATAGAAGAGC / PCR sequencing
TLP10-cds3-F/R / CTTCCCGTCATCGCTCAA / AGAGGTTGGCGGTAATCC / PCR sequencing
OsTLP12 / AK106853 / TLP2-RT-F/R / CTAATTTCACGGGAACAAAGTTCAC / GCTGAACGCCTTAGCACCTT / Real-time PCR
OsTLP13 / EU328285 / TLP4-RT-F/R / GTCGGATCCAGATACCGAATCT / GCGTAGTAACAGTAGGCGCAAA / Real-time PCR
TLP4-cds1-F/R / CCCCACAATTTGAATAAGACA / CACCACGAACTCCGACCT / PCR sequencing
TLP4-cds2-F/R / CTACTCCCTCTACACCAACGA / TTTCCACCAATCCAAATGA / PCR sequencing
TLP4-cds3-F/R / GAAGAAGCCGCCTGTGGT / TGCCTTCCTTTCTCCTCCA / PCR sequencing
TLP4-F/R / TTTGAAGTTTGCGGGTGC / AGACGGCGACCGTTACCAC / PCR sequencing
OsTLP14 / AK102370 / TLP6-RT-F/R / GCCAATGGAACTCGCAAAAA / GATGCGGTTCATCTGATTGTGA / Real-time PCR
Actin / X15865 / Actin-F/R / TGTATGCCAGTGGTCGTACCA / CCAGCAAGGTCGAGACGAA / Real-time PCR

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Supplemental Table 2. Amino acid similarity among OsTLP proteinsa

OsTLP
member / OsTLP member
1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / 9 / 10 / 11 / 12 / 13 / 14
1 / 100 / 71 / 72 / 75 / 83 / 57 / 58 / 56 / 50 / 52 / 41 / 48 / 22 / 42
2 / 100 / 79 / 67 / 68 / 50 / 56 / 54 / 48 / 50 / 38 / 47 / 21 / 40
3 / 100 / 68 / 67 / 51 / 53 / 51 / 47 / 48 / 37 / 44 / 18 / 39
4 / 100 / 70 / 63 / 58 / 55 / 50 / 50 / 39 / 46 / 22 / 42
5 / 100 / 52 / 56 / 55 / 49 / 50 / 39 / 46 / 22 / 42
6 / 100 / 46 / 41 / 44 / 42 / 36 / 38 / 16 / 64
7 / 100 / 60 / 57 / 58 / 47 / 55 / 28 / 58
8 / 100 / 53 / 54 / 43 / 52 / 28 / 47
9 / 100 / 77 / 76 / 69 / 33 / 47
10 / 100 / 64 / 74 / 32 / 44
11 / 100 / 57 / 27 / 40
12 / 100 / 34 / 42
13 / 100 / 23
14 / 100

aFor the OsTLP gene encoding multiple proteins, only the sequence of the largest protein is compared with other OsTLP proteins.

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