Supplemental Table 1: Primers used for mapping and vector construction. Mapping primers are presented as pairs, and position on BAC is indicated.

Primer name / Forward primer / Reverse primer / BAC / position
1-15 / GCA GCA TAG AGG CGA GGT TA / GGT GGA TCG ACT GTG AGG TT / F7A19 / 94232
1-19 / TCA TCA ACC TCA TCG CAG TC / GGA AGA GGA AGC TCA CGA TG / F20D23 / 10784
1-20 / CAA GGA TTG CAA CAA CAA CAA / GCC CAT CAC GTA GAA GGA GA / F15H18 / 93733
1-21 / GGC AAT CAA TTA GCC GTC AT / TTT TCT TTG CCA AAC / F18014 / 49745
1-21A / ACG AGG CTT ATC GTC ATT CC / GAC TGG GAT AGC GAC ATG GT / F18014 / 65610
1-21B / GAA ATC TGT GTG AAA GCT GCT G / GAA CGG ACC TAA AAC GAC AAA C / F14P1 / 88154
1-21 bII HpaII CAPS / GCC CTT CAA CGA AAT TGT TT / GCG GGT TTC TGT ACG GAC TA / F14P1 / 81572
1-21C / AAC AGA ATC TCT CGC CAT TTT C / TGC TAA CAT CCC ACT CCT CA / F14P1 / 51666
1-21 cII DdeI / ATT TGG AGT GGA TGA TGT TGT G / GTT GGA TAA TCC CAA AGG TCA A / F14P1 / 30279
1-21D / CTT TAC AAG GCC CAT AAA GCT AA / CTG CAT TGC CAA TAG T / F14P1 / 17470
1-21 dII HpyCH4IV CAPS / ATT CAA CGA CGT AAG AAT CGA G / CAT ATG AAC ATT GGG TGG TTT G / F6F9 / 39439
1-21 dIII HinfI CAPS / GCA GAT GTT TCG TCT CCT CTC / GTG CAA TGC CCT CAT AGT TAC A / F6F9 / 28251
1-21 dIII DdeI dCAPS / TTC AAT CTA AAG AGC TGG CTT TG / ATT CTT TTT CCA GGA AAA TTG AC / F6F9 / 28251
1-21 dIV / ACT GAT CCA ATA AGA A / AGC TGG AGA TCT TCC CAT / F6F9 / 24162
1-21E / AGT ATG AGC CGC GTA CGA TT / TGG TTT CCA GAT AAA TCG ATG / F6F9 / 1636
1-21 eII HpyCH4IV dCaps / AGA TGA CTG AAG CTG CTC TTG A / TAA GTT AAA GAG GAA AGA T / T20H2 / 77872
1-21 eII Xholl CAPS / TCA GCC ATC TTA GCC TTT AAC C / GCG TGG AAT CAG TTC AAA TGT A / T20H2
1-21 eIII DdeI dCAPS / CGA TCA ATC AAA TCG TCT TAT TT / TGC TAA CAT CCC ACT CCT CA / T20H2 / 72261
1-21F / TTG GTC GGC TTC AAG AAA AA / CCT CCA ACT CCA AGC / T20H2 / 66510
1-21G / CGA CCC TCG AGA AAG AAC AA / TCA ATG AAT GCT ACA ATG / T20H2 / 36893
1-22 / TGC TAA CAT CCC ACT CCT CA / TCT GGT GCT GCA TTT CTG TC / T20H2 / 38374?
1-23 / TTTCGTCCTGCCCAATAAAG / TGTGACGTGGAAAAAGTGAA / F9H16 / 52878
1-24 / CAAGGGAAGAGACAAACGAGA / CCAAATATTTCCATGGTGTGG / T26F17 / 74927
1-26 CER464619 / ACG GGT TTA CGT TTT TGG TG / CAA GCG ATG CAA TTG AGA AA / F12K8 / 22427
1-29 / TGCATGCAATTATCACGTATG / GCGGCACAACCTAAATGAAA / F21J9 / 77494
1-31 CER459662 / GCA AGA ACA TCT TGG AAA ATC A / TCC CTT AGA GTG GCG AAT GT / T24P13 / 95027
BDLcDNARPNotI / GGC GGC CGC CTA AAC AGG GTT GTT TCT TTG TC
SalIBDLcDNALP / GGT CGA CCC ATG CGT GGT GTG TCA GAA TTG G
SalIMPcDNALP / GGT CGA CCC ATG ATG GCT TCA TTG TCT TG
abncDNARPNotI / GGC GGC CGC TTA TGG GTG AGT TAG TAG TCC T
MPcDNARPNotI / GGC GGC CGC TTA TGA AAC AGA AGT CTT AAG

Supplemental Figure 1: Cleared embryos from days post anthesis (DPA) staged siliques of wild type plants (a-c) and non-staged elongating siliques of mpabnplants grown at 22oC. Representative octant (a, d), Globular (b, c) and heart (c, f) stage embryos are shown. Viewed under DIC optics.