Supplemental File S8 - Effects of Mutations on Proteins G Example Results

Supplemental File S8 - Effects of Mutations on Proteins – G Example Results.docx

(*: stop codon, -: deleted nucleotide , underlined: added nucleotide)

Name aa Sequence

Initial 7 nt ATG TGT TTG ACG CAA CAG CGA TAA AGC TTG AAC CGT AAT TGA

aa M C L T Q Q R *

I1a: 13 nt ATG TGT AAAA TTG ACG CAA CAG CGA TAA AGC TTG AAC CGT AA

aa M C K I D A T A I K L E P *

I1b: 1 nt ATG TGA

aa M *

I2a: 12 nt ATG TGT TTG ACG CAA CAG CGA AAAAAAAA TAA AGC TTG A

aa M C L T Q Q R K K N K A *

I2b: 2 nt ATG TGT TAA

aa M C *

I3a: 10 nt ATG TGT TTG AC AAAAAAAAA G CAA CAG CGA TAA

aa M C L T K K K Q Q R *

I3b: 7 nt ATG TGT TTG ACG CAA CAG AAATAG

aa M C L T Q Q K *

I3c: 9 nt ATG TGT TTG ACG AAAAAA CAA CAG CGA TAA

aa M C L T K K Q Q R *

I3d: 6 nt ATG TGT TTG ACG C TTTTTT AA

aa M C L T L F *

I3e: 7 nt ATG TGT TTG ACG CAA CAG CGA T AAAAAAAAAAAA AA…

aa M C L T Q Q R *

D1a: 8 nt ATG TGT --- -CG CAA CAG CGA TAA AGC TTG A

aa M C R N S D K A *

D1b: 2 nt ATG TGT -TG A

aa M C *

D2a: 11 nt ATG TGT TTG ACG CAA C-- CGA TAA AGC TTG AAC CGT AA

aa M C L T N P I K L E P *

D2b: 1 nt ATG TG------A

aa M *

D3a: 6 nt ATG TGT TTG ACG CA- --G CGA TAA

aa M C L T Q R *

D3b: 6 nt ATG TG- --G ACG CAA CAG CGA TAA

aa M W T Q Q R *

D3c: 5 nt ATG TGT ------CAA CAG CGA TAA

aa M C Q Q R *

D3d: 1 nt ATG TG------A

aa M *

D3e: 11 nt ATG TGT TTG ACG CAA CAG CGA T------TG AAC CGT AAT TGA

aa M C L T Q Q R L N R N *