Supplemental Data II. Summary of wheat cDNA clones isolated by yeast two-hybrid screening

Bait Protein / Library
screened / Prey protein / Clones selected by PCR
and restriction analysis / +ve clones tested
for self-activation / Length of clones / Full length clone
isolation
TaRSZ38, full-length CDS / WGL / TaTra2 / 2 / 2 / Partial CDS with 3’UTR / From WGL by PCR
TaLuc7 / 15 / 2 / Partial CDS with 3’UTR / From WEMBL by PCR
TaU1-70kD / 7 / 2 / Partial CDS with 3’UTR / From WEMBL by PCR
TaU2AF65 / 19 / 2 / Full length CDS / -
TaU2AF65a / 7 / 2 / Full length CDS / -
TaPrp38 / 3 / 2 / Partial CDS with 3’UTR / From WEMBL by PCR
TaTRN-SR / 39 / 2 / Partial CDS (approx 2/3) / From WEMBL by PCR
TaRSZ38BP1 / 2 and 1 / 1 / Full length and partial length CDS / -
WENDL / TaU1-70kD / 2 / 1 / Partial CDS with 3’UTR / -
TaPrp38 / 53 and 4 / 1 and 1 / Partial CDS with 3’UTR (two different lengths) / -
TaLuc7 / 3 and 1 / 1 and 1 / Partial CDS with 3’UTR (two different lengths) / -
TaTra2, full-length CDS / WGL / TaSRp30 / 4 and 6 / 2 / Full and partial length CDS / -
TaSRp30a / 51 / 2 / Partial CDS with 3’UTR / From WEMBL by PCR
TaRSZ22 / 20 and 25 / 2 and 2 / Two full length homoeologues / -
TaRSZ22a / 30 and 24 / 2 and 2 / Two full length homoeologues / -
TaTRN-SR / 3 / 1 / Partial CDS (approx 2/3) / -
TaTRN-SR, partial CDS / WGL / TaU2AF35 / 3 / 2 / Full length CDS / -
TaU2AF65a / 2 / 2 / Full length CDS / -
TaY14 / 58, 7 and 4 / 2 / Full length CDS and two partial length CDS / -
TaDRH1 / 3 / 2 / Partial sequence, C-terminal end / Longer partial sequence from WEMBL by PCR

WGL, wheat grain library; WENDL, wheat endosperm library; WEMBL, wheat embryo