AOAC 2010

Summary Report for the

Veterinary Sub-Group Meeting

Chair:Dr. Sherri Turnipseed, Food and Drug Administration, USA

Secretary: Dr. Perry Martos, University of Guelph, Canada

On September 27, 2010, the Chemical Contaminants & Residues in Food Community met to discuss various priority issues. The veterinary sub group’s primary task at this time was to develop an action plan on “veterinary drugs in seafood under consideration for collaboration”.

This report summarizes highlights from the subsequent veterinary sub-group meeting held on September 28, 2010.

  1. Focus
  2. The Chair was charged with the responsibility to develop a current perspective on submitted veterinary drug residue methods specific to seafood matrices. While a number of methods were submitted, the Chair also carried out a mini literature review, focusing on dyes, nitrofurans and multi-residue methods in seafood. Included are phenicols, fluoroquinolones, quinolones and methyltestosterone.
  3. The goal to narrowing down the best candidate methods included the possibility to parse out sound attributes from one method and combine them with aspects from other methods, thereby resulting in a third, “synthesized” method providing the “best of both worlds”.
  4. A stakeholder representative suggested the need for testing with a confirmatory method and potentially “as close to the source as possible”. In addition, a focus was made on the study of catfish, shrimp, tilapia, and salmon.
  5. The stakeholder representative also suggested the use of “lateral flow technology”.
  6. The charge statement was that we need methods / approaches suitable to test fish that are intended for processing.
  7. The methods
  8. Submitted and selected methods from the Chair’s activities were summarized in tabular form and provided, in confidentiality, to the veterinary sub-group. Approximately 25-30 methods were available for consideration. Among the methods included draft manuscripts, manuscripts in review, manuscripts in press, and published, peer-reviewed articles. Some of the methods were directly related to analyses in seafood, while other methods were focused, for example, on bovine.
  9. Single analyte methods and multi-residue methods were discussed at length. In particular, nitrofurans garnered considerable attention due to the complexity in their analyses.
  10. Certainly, specific issues with compound classes such as nitrofurans created clarity that those classes are not amenable to analysis with multi-residue methods.
  11. Success with multi-residue methods is often realized through capturing as many compounds as possible with one routine and avoiding sample preparation techniques such as hydrolysis or enzymatic pre-treatments.
  12. General issues
  13. There was considerable discussion on nitrofurans, and some focus to multi-residue methods and methods for dyes.
  14. One of the topics discussed was the need to “develop the best science” in support of the stakeholder needs, but that the scientific objectives needs to be clearly defined and subsequently proven by way of validation and collaborative study.
  15. Another issue discussed were the method performance criteria, where some discussed what spiking levels would be important.
  16. The point about the need for Study Directors was brought up several times, highlighting the need to ensure there is at least one Study Director for every collaborative study.
  17. The breakout groups
  18. Three working groups were formed following direction from the Chair. Individuals within those groups are highlighted below. The groups met separately and discussed the various methods provided to them, and were asked to provide written recommendations as to which “approaches” would be best suited to meet the stakeholder’s needs. Those will be provided separately from this communication.
  19. Nitrofurans
  20. Eric Verdon, Anses
  21. Rolando Perez, ADPEN
  22. Haejun An, US FDA
  23. Teresa Cain, US FDA
  24. Dyes
  25. John Reuther, Eurofins
  26. Olga Shimelis, Supelco
  27. Steve Lehotay, USDA/ARS
  28. Eric Verdon, Anses/France
  29. Multi-residue
  30. Perry Martos, U. of Guelph
  31. Eric Verdon, Anses
  32. Joe Boisson, CFIA
  33. John Reuther, Eurofins
  34. Thierry Delatour, Nestle
  35. Rolando Perez, ADPEN

Page 1 of 1