Standard Operating Procedures s23

Standard Operating Procedures

Laboratory Specific

Chemical: 4,4'-Methylenebis(2-chloroaniline) (MBOCA)

Please fill out the form completely. Print a copy and insert into your

Laboratory Safety Manual and Chemical Hygiene Plan.

Refer to instructions for assistance.

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Department:______Date when SOP was written:______

Date when SOP was approved by the lab supervisor: ______

Principal Investigator:______

Internal Laboratory Safety Coordinator/Lab Manager:______

Laboratory Phone:______Office Phone:______

Emergency Contact:______

(Name and Phone Number)

Location(s) covered by this SOP:______

(Building/Room Number)

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Type of SOP: Process Hazardous Chemical Hazardous Class

Purpose

4,4'-Methylenebis(2-chloroaniline) (MBOCA) is a substance used as a curing agent in polyurethane production. It is a suspected human carcinogen.

It is a weak base with a slight odor and is reactive to active metals such as sodium, potassium, magnesium and zinc.

Physical & Chemical Properties/Definition of Chemical Group

CAS#: 101-14-4

Class: Regulated Carcinogen

Molecular formula: C13H12Cl2N2

Appearance: off-white powder

Molar mass: 267.15 g/mol

Density: 1.354g/cm3

Melting point: 102-107°C

Boiling point: 412°C @ 760mmHg

Solubility in water: insoluble

Potential Hazards/Toxicity

Based on available data for 4,4'-Methylene bis (2-chloroaniline) repeated exposures may produce cyanosis and cancer. May effect kidney function. The following have been observed in experimental animals only: Pulmonary carcinoma, malignant liver tumors, carcinoma of the breast and bladder and methemoglobinemia.

INHALATION: May produce cyanosis. 4,4'-Methylenebis-2-chloroaniline may cause cancer based on tests in laboratory animals. May cause respiratory sensitization in susceptible individuals. At room temperature, vapors are minimal due to low vapor pressure. If heated, excessive concentrations are attainable that could be hazardous on single exposure.

EYE CONTACT: Vapors from heated product may cause eye irritation and severe damage.

SKIN CONTACT: Hazardous components are absorbed through the skin. 4,4'-Methylenebis-2-chloroaniline may cause cancer based on tests in laboratory animals. May produce cyanosis. May cause allergic skin reaction in susceptible individuals. Prolonged or repeated exposure may cause skin irritation.

INGESTION: Material may be ingested through poor hygiene practices. May cause gastrointestinal, kidney, and central nervous system damage. May produce cyanosis. 4,4'-Methylene bis-2-chloroaniline single oral dose toxicity: Oral LD50 for rats is 2,100 mg/kg.

TARGET ORGANS: May effect kidney function.

CHRONIC: Based on available data for 4,4'-Methylene bis (2-chloroaniline) repeated exposures may produce cyanosis and cancer. The following have been observed in experimental animals only: Pulmonary carcinoma, malignant liver tumors, carcinoma of the breast and bladder and methemoglobinemia.

Monitoring

Special urinalysis should be conducted to determine that no employee's urine contains >100 mg of MBOCA/liter of urine when the specific gravity (SG) is adjusted to 1.024. When urine sample are not received, the exposure shall not exceed an 8-hour time weight average (TWA) concentration of MBOCA in excess of 10 r/m3 of air and employee shall not be exposed to a ceiling concentration in excess of 50 rg/m3 during any 15 minute period. Accessible surfaces which employees are likely to contact in controlled access areas during their normal work shall not exceed 100 rg MBOCA/100 cm2 of surface. The average of at least five wipe samples shall be used.

If one sample exceeds the mean by a factor of 10 or more, it will be rejected as not being representative. Use of >1 kg of MBOCA, irrespective of concentration, dilution, or form, except frozen pre-mix, in any 6-month period, a program of surface-contamination monitoring and urinalysis (for MBOCA) should be instituted. Any employee entering a controlled-access area will have the urinalysis repeated quarterly with samples to be collected near the end of the work shift. Any employee whose urinary MBOCA level exceeds 100 rg/1 with SG adjusted 10 1.024, shall be tested at least each month until two consecutive samples taken not <3 days apart have been reduced to <100 rg/1. The method of monitoring and measurement shall be accurate and precise so that 95% of the determination are within 25% of the true value at the exposed limit. Employees are to be notified in writing, if exposed in excess of PEL, within 10 days of the numerical results of the monitoring.

Personal Protective Equipment (PPE)

· All persons shall wear personal protective equipment when handling 4,4'-Methylenebis(2-chloroaniline). This includes wearing a lab coat, nitrile gloves, closed toe shoes, and chemical splash goggles when working with 4,4'-Methylenebis(2-chloroaniline). Gloves should be changed frequently.

· Leave lab coats, gloves, and other personal protective equipment in the lab once your work is complete to prevent the spread of this or other chemicals outside of the lab.

Engineering Controls

Always handle 4,4'-Methylenebis(2-chloroaniline) in a certified chemical fume hood. Use dry materials in a fume hood, or choose premixed solutions to avoid inhalation exposure.

First Aid Procedures

· Inhalation or Ingestion: In the case of 4,4'-Methylenebis(2-chloroaniline) ingestion, obtain medical attention immediately.

· Eyes exposure: Immediately flush eyes with copious amounts of water for at least 15 minutes, preferably in an emergency eyewash.

· Skin exposures: In the event of skin exposure, remove contaminated clothing and immediately wash the affected area with soap and copious amounts of water for 15 minutes.

Special Handling and Storage Requirements

1. Signage is required for the area where 4,4'-Methylenebis(2-chloroaniline) is stored. Signage should read “Controlled Access Area Authorized Personnel Only”.

1. All work with 4,4'-Methylenebis(2-chloroaniline) is to be done in an " 4,4'-Methylenebis(2-chloroaniline)" designated area in order to keep 4,4'-Methylenebis(2-chloroaniline) contamination to a minimum. Any persons in this area are required to wear personal protective equipment. Safety shower and eye wash stations should be easily accessible where 4,4'-Methylenebis(2-chloroaniline) is used.

2. All laboratory equipment (such as beakers, pipettes, etc.) used in the "4,4'-Methylenebis(2-chloroaniline)" designated area are to be labeled as

“CAUTION 4,4'-Methylenebis(2-chloroaniline). Avoid skin contact. Avoid breathing dust/vapor of this regulated carcinogen”.

3. Wash thoroughly after handling. Remove contaminated clothing and wash before reuse. Use with adequate ventilation (fume hood). Minimize dust generation and accumulation. Do not get in eyes, on skin, or on clothing.

4. Keep container tightly closed. Avoid breathing dust.

5. Store in a tightly closed container. Store in a cool, dry, well-ventilated area away from incompatible substances. Containers should have a “cancer hazard” label and be placed in a designated area with secondary containers labeled “cancer hazard”.

Spill and Accident Procedure

Chemical Spill Dial 911 and x59797

Spill – Help contaminated or injured persons. Evacuate the spill area. Avoid breathing vapors. Eliminate sources of ignition if the chemical is flammable. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).

Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.

Large (>1 L) – Dial 911 (or 310-825-1491 from cell phone) and EH&S at x59797 for assistance.

Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for 15 minutes by forcibly holding the eye open. Seek medical attention. Notify supervisor and EH&S at x59797 immediately.

Medical Emergency Dial 911 or x52111

Life Threatening Emergency, After Hours, Weekends And Holidays – Dial 911 (or 310-825-1491 from cell phone) or contact the Ronald Reagan UCLA Medical Center (emergency room) directly at x52111 (located at 757 Westwood Plaza, enter from Gayley Avenue). Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Non-Life Threatening Emergency– Go to the Occupational Health Facility (OHF), x56771, CHS room 67-120 (This is on the 6th floor, 7th corridor, room 120. Enter through the School of Dentistry on Tiverton Drive and proceed to the “O” elevator to the 6th floor.)Hours: M - F, 7:30 a.m. to 4:30 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All serious injuries must be reported to EH&S at x59797 within 8 hours.

Needle stick/puncture exposure (as applicable to chemical handling procedure)– Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 231 from a campus phone, enter 93333 when prompted and then enter your extension. Hours: M – F, 8:00 a.m. to 4:00 p.m. At all other times report to Ronald Regan UCLA Medical Center (emergency room) at x52111. Note: All needle stick/puncture exposures must be reported to EH&S at x59797 within 8 hours.

Decontamination/Waste Disposal Procedure

Waste disposal procedures

1. All 4,4'-Methylenebis(2-chloroaniline) contaminated waste shall be disposed of into waste containers specifically designated for 4,4'-Methylenebis(2-chloroaniline) waste.

2. No waste streams containing 4,4'-Methylenebis(2-chloroaniline) shall be disposed of in sinks.

3. Wash hands and arms with soap and water after finished.

4. Once the waste container is full, dispose of according to the UCLA EH&S hazardous waste guidelines.

Waste containers containing 4,4'-Methylenebis(2-chloroaniline) (a Particularly Hazardous Substance) must be labeled:

“DANGER, CANCER HAZARD”

Material Safety Data Sheet (MSDS) Location

(State the location of MSDS)

Hardcopy or electronic copy must be available.

Online MSDS can be accessed at http://msds.ehs.ucla.edu.

Protocol/Procedure

(Add specific description of procedure.)

Note: Any deviation from this SOP requires written approval from PI.

Documentation of Training (signature of all users is required)

· Prior to conducting any work with 4,4'-Methylenebis(2-chloroaniline), designated personnel must provide training to his/her laboratory personnel specific to the hazards involved in working with this substance, work area decontamination, and emergency procedures.

· The Principal Investigator must provide his/her laboratory personnel with a copy of this SOP and a copy of the 4,4'-Methylenebis(2-chloroaniline)’s MSDS provided by the manufacturer.

· The Principal Investigator must ensure that his/her laboratory personnel have attended appropriate laboratory safety training or refresher training within the last two years.

I have read and understand the content of this SOP:

Name Signature Date

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