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Electronic Supplementary Material

Article title: Spatial dynamics of pollination in dioecious Shepherdia canadensis (Elaeagnaceae)

Journal name: Plant Ecology

Author names: Shang-Yao Lin, Erica Nol, Marcel E. Dorken

Affiliation and e-mail address of the corresponding author:

S.-Y. Lin (corresponding author)

Department of Biology, University of Ottawa, 30 Marie Curie, ON, K1N 6N5, Canada.

e-mail:

Appendix

Appendix 1

Table S1. Mean (± SE) shrub size, flower production, fruit production, percent fruit set, and stigmatic pollen load of males and/or females in each study site. Fruit set represented the percentage of total flowers that yielded berries and was obtained from a subset of flowering females.

Site / Shrub height (cm) / Shrub crown area (cm2) / Flower production in 2011 (number of flowers per shrub) / Female fruit production (number of berries per shrub) / Fruit set (%) / Stigmatic pollen load (number of pollen grains per flower per plant)
Male / Female / Male / Female / Male / Female / 2011 / 2012 / 2011 / 2012
1 / 44.1 ± 3.0 (n = 34) / 51.0 ± 3.2 (n = 26) / 40.3 ± 8.0 (n = 34) / 58.6 ± 14.2 (n = 26) / 104.2 ± 19.8 (n = 10) / 101.6 ± 40.5 (n = 8) / 159.0 ± 72.7 (n = 20) / 21.6 ± 12.7 (n = 20) / 46.2 ± 6.4 (n = 8) / 7.1 ± 2.0 (n = 19)
2 / 35.9 ± 2.6 (n = 34) / 41.6 ± 2.3 (n = 26) / 38.7 ± 8.2 (n = 34) / 36.2 ± 8.1 (n = 26) / 323.2 ± 34.8 (n = 10) / 207.7 ± 28.2 (n = 9) / 95.2 ± 29.2 (n = 20) / 86.4 ± 21.1 (n = 20) / 30.8 ± 7.1 (n = 8) / 32.4 ± 4.6 (n = 20)
3 / 37.4 ± 4.8 (n = 20) / 33.7 ± 1.9 (n = 40) / 28.6 ± 9.6 (n = 20) / 21.8 ± 4.2 (n = 40) / 140.3 ± 12.0 (n = 6) / 129.4 ± 36.9 (n = 12) / 143.3 ± 47.7 (n = 21) / 21.0 ± 6.6 (n = 20) / 49.2 ± 5.5 (n = 12) / 16.8 ± 3.6 (n = 20)
4 / 46.0 ± 3.2 (n = 37) / 52.9 ± 2.6 (n = 23) / 37.0 ± 8.7 (n = 37) / 38.8 ± 7.4 (n = 23) / 334.6 ± 47.9 (n = 15) / 230.0 ± 92.8 (n = 4) / 193.6 ± 57.3 (n = 20) / 44.5 ± 11.1 (n = 20) / 58.0 ± 3.0 (n = 4) / 18.0 ± 3.8 (n = 24)
5 / 37.8 ± 2.3 (n = 36) / 39.2 ± 2.3 (n = 24) / 24.5 ± 4.2 (n = 36) / 27.3 ± 9.2 (n = 24) / 168.4 ± 16.4 (n = 12) / 165.6 ± 47.3 (n = 8) / 65.1 ± 15.4 (n = 21) / 16.7 ± 4.9 (n = 20) / 45.9 ± 7.0 (n = 8) / 19.6 ± 4.5 (n = 20)
6 / 41.5 ± 3.2 (n = 35) / 40.1 ± 3.1 (n = 25) / 32.7 ± 10.5 (n = 35) / 22.6 ± 6.2 (n = 25) / 259.7 ± 44.1 (n = 13) / 232.6 ± 54.2 (n = 7) / 82.9 ± 18.1 (n = 20) / 81.6 ± 36.0 (n = 21) / 47.2 ± 6.3 (n = 7) / 8.9 ± 1.7 (n = 19)
7 / 48.0± 4.2 (n = 31) / 47.0 ± 3.3 (n = 29) / 45.6 ± 15.6 (n = 31) / 53.0 ± 9.9 (n = 29) / 200.2 ± 39.0 (n = 13) / 143.3 ± 46.1 (n = 6) / 130.4 ± 44.3 (n = 20) / 31.6 ± 9.4 (n = 20) / 45.5 ± 9.4 (n = 6) / 16.8 ± 3.9 (n = 19)
8 / 35.2 ± 2.9 (n = 26) / 42.4 ± 2.9 (n = 34) / 26.3 ± 7.6 (n = 26) / 39.0 ± 8.3 (n = 34) / 217.3 ± 73.7 (n = 6) / 179.6 ± 46.4 (n = 10) / 125.0 ± 35.4 (n = 21) / 36.6 ± 8.7 (n = 20) / 44.2 ± 7.1 (n = 10) / 14.8 ± 2.8 (n = 16)
9 / NA / 46.0 ± 2.4 (n = 20) / NA / 25.8 ± 4.9 (n = 20) / NA / NA / NA / 12.2 ± 5.9 (n = 20) / NA / 23.9 ± 4.0 (n = 57)
10 / NA / 30.3 ± 1.8 (n = 20) / NA / 15.3 ± 2.1 (n = 20) / NA / NA / NA / 0.3 ± 0.2 (n = 20) / NA / 9.3 ± 2.3 (n = 34)

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Appendix 2

Table S2. Abundance and richness of insects observed visiting S. canadensis flowers in each site in 2011.

Site
Visitor group / 1 / 2 / 3 / 4 / 5 / 6 / 7 / 8 / Abundance per group
Miridae / 1 / 1
Empididae / 1 / 1 / 2
Sciaridae / 1 / 1
Syrphidae / 2 / 9 / 2 / 1 / 14
Unidentified Dipteran / 1 / 1
Formicidae / 10 / 1 / 2 / 1 / 12 / 26
Apoidea / 2 / 2
Abundance per site / 15 / 1 / 11 / 4 / 4 / 0 / 0 / 12 / 47
Family richness per site / 5 / 1 / 3 / 2 / 3 / 0 / 0 / 1 / 7

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Appendix 3

Fig S1. Distribution of males (opened circles) and females (closed circles) at site: 2 (a) and site 6 (b).

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Appendix 4

Figure S2. An example of the relationship between male density and fruit set at distance class 4 in Site 2 (Pearson correlation, t = 2.20, R = 0.47, P = 0.04, nplants = 18).