Immunohistochemistry: A Brief Review

*Author1, Author2……..

1Affiliation of first author, 2Affiliation of second author

*Contact Author e-mail:

Abstract

Immunohistochemistry (IHC) is a technique for identifying cellular or tissue constituents (antigens) by means of antigen- antibody interactions. It is useful as an auxillary diagnostic procedure in histopathology. The introduction of prognostic and predictive markers in IHC has made a tremendous impact on patient’s treatment and management. These methods are particularly useful to a pathologist in ambiguous areas of neoplastic process enhancing the accuracy to large extent. This review provides a summary of merits, demerits, staining procedures and troubleshooters in immunohistochemistry technique. [150 to 200 words]

Key Words: Immunochemistry, Monoclonal Antibodies, Amino Acids, Polyclonal, Epitopes.

RUAS-JDOR Journal 1 Vol. 10, Issue 1

1.  Introduction

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1.1  Structure and Description:

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Follow this order when typing manuscripts: Title, Authors, Affiliations, Abstract, Keywords, Main text, Acknowledgements, Appendix and References. The pictures and tablesmust be placed at appropriate positions in the text.(body of text in TNR, 9)

Units


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1.2  Guidelines for Manuscript Preparation

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Statistics

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1.4  Tables

Tables must be constructed using the table-tool available in MS-Word only as shown in Table 1. Do not include image of tables created in other applications. Tables must be numbered sequentially.

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·  Include the units of measurements in the table text wherever the variable list is used

Table 1. Table showing percentage of advise requests on oral health reported by pharmacists

Sl. No. / Enquiry (E) / Percentage (%)
1 / Ulcers / 20%
2 / Sore Mouth / 12%
3 / Bleeding Gums / 14%

1.5  Figures

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Fig. 3 Text organization of published articles in the British Medical Journal from 1935 to 1985 (n=341)

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ACKNOWLEDGMENT

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Include references at the end of the paper. The referencing style for journal papers, conference papers, textbooks and websites are given below.

References

[Print Articles]

Hallal AH, Amortegui JD, Jeroukhimov IM, Casillas J, Schulman CI, Manning RJ, et al. Magnetic resonance cholangiopancreatography accurately detects common bile duct stones in resolving gallstone pancreatitis. J Am Coll Surg. 2005 Jun;200(6):869-75.

[Electronic Journal Article]

Stockhausen L, Turale S. An explorative study of Australian nursing scholars and contemporary scholarship. J Nurs Scholarsh [Internet]. 2011 Mar [cited 2013 Feb 19];43(1):89-96. Available from: http://search.proquest.com. ezproxy.lib.monash.edu.au/ docview/858241255?accountid=12528

[Electronic Journal Articles with DOI]

Kanneganti P, Harris JD, Brophy RH, Carey JL, Lattermann C, Flanigan DC. The effect of smoking on ligament and cartilage surgery in the knee: a systematic review. Am J Sports Med [Internet]. 2012 Dec [cited 2013 Feb 19];40(12):2872-8. Available from: http://ajs.sagepub.com/content/40/12/2872 DOI: 10.1177/0363546512458223

[Books and Other Monographs Book Reference]

[Print book]

Carlson BM. Human embryology and developmental biology. 4th ed. St. Louis: Mosby; 2009. 541 p.

[Electronic Book]

Shreeve DF. Reactive attachment disorder: a case-based approach [Internet]. New York: Springer; 2012 [cited 2012 Nov 2]. 85 p. Available from: 5 http://ezproxy.lib.monash.edu.au/ login?url=http://dx.doi.org/10.1007/978-1-4614- 1647-0

[Chapter in an Edited Book]

Blaxter PS, Farnsworth TP. Social health and class inequalities. In: Carter C, Peel JR, editors. Equalities and inequalities in health. 2nd ed. London: Academic Press; 1976. p. 165-78

[Chapter in an Edited Electronic Book]

Halpen-Felsher BL, Morrell HE. Preventing and reducing tobacco use. In: Berlan ED, Bravender T, editors. Adolescent medicine today: a guide to caring for the adolescent patient [Internet]. Singapore: World Scientific Publishing Co.; 2012 [cited 2012 Nov 3]. Chapter 18. Available from: http://www.worldscientific.com/ doi/pdf/10.1142/9789814324496_0018

[Dissertations]

Tjerbo T. The multilevel governance of health care: an analysis of the Norwegian health care sector [doctoral thesis]. Oslo: University of Oslo, Faculty of Medicine; 2009.

[Web Page]

[Home Page]

Diabetes Australia. Diabetes globally [Internet]. Canberra ACT: Diabetes Australia; 2012 [updated 2012 June 15; cited 2012 Nov 5]. Available from: http://www.diabetesaustralia.com.au/en/ Understanding Diabetes/DiabetesGlobally/

[Part of Website]

Australian Medical Association [Internet]. Barton ACT: AMA; c1995-2012. Junior doctors and medical students call for urgent solution to medical training crisis; 2012 Oct 22 [cited 2012 Nov 5]; [about 3 screens]. Available from: https://ama.com.au/media/junior-doctors -and-medical-students-call-urgentsolution-medical-training-crisis

[Conference]

Nørvåg K. Space-Efficient Support for Temporal Text Indexing in a Document Archive Context. In: Koch T, Sølvberg I, editors. Research and Advanced Technology for Digital Libraries. 7th European Conference, ECDL; 2003 Aug 17-22; Trondheim, Norway. Berlin: Springer; 2003. p. 511-22.

[Software Manual]

Epi Info [computer program]. Version 6. Atlanta (GA): Centers for Disease Control and Prevention; 1994.

[Patent]

Pagedas AC, inventor; Ancel Surgical R&D Inc., assignee. Flexible endoscopic grasping and cutting device and positioning tool assembly. United States patent 20020103498. 2002 Aug 1.

RUAS-JDOR Journal 4 Vol. 10, Issue 1