Assessor / Advising Health and Safety Officer: / Date Completed: / Date of Previous Form if Any: / Date Reviewed: (additions, amendments to be added) / Authorised personnel / Sheet Number
Ewan McGhee / Richard Selkirk / 05/03/2014 / N/A / 04/03/2015 / Trained users / 1 of
HAZARD POTENTIAL of Chameleon Ultra II laser use
(to be numbered) /
SIGNIFICANT RISKS TO HEALTH AND SAFETY
Identify if any category of employee is especially at risk. Also identify if any category of non- employee is at risk. / PREVENTIVE AND PROTECTIVE MEASURES TO REDUCE RISKS TO AN ACCEPTABLE LEVEL (OR AVOID THEM ALTOGETHER), AND TO COMPLY WITH RELEVANT HEALTH AND SAFETY LAWHazard / Nature of Hazard/Person at risk / Control Measure(s)
Chameleon Ultra II laser + OPO
1. Optical / Operator at risk from exposure to pulsed NIR light / PPE in form of NIR laser blocking goggles, user training, appropriate beam enclosure
2. Environment / Exposure to pulsed NIR laser light to un-authorised people / Visitors are always accompanied, laser warning sign in corridor, laser interlock system
3. Electrical / Risk of electric shock for user / No user access to internals of power supply
4. Mechanical / Engineers and approved users risk of injury from moving it / Only moved in pairs of workers, secured to optical bench all other times
Trim-scope II
1. Optical / Operator at risk from eye damage caused by UV Light and NIR light via eyepieces / Blocking filters inserted in light path
2.Electrical / Risk of electric shock for user / Check for integrity of cables and electrical components
3. Biohazard / Risk of system and user contamination from biological samples imaged / Covered dishes used for live cell work, sealed coverslips for fixed samples, use of gloves at all times (location requirement as well)
4. Chemical / Risk of user and system chemical contamination from chemical treatments used in imaging experiments / Gloves and appropriate PPE
Background Information
Describe the product and application / LaVisionBiotec Trim-scope multi-photon microscope system consisting of: mercury bulb light source, an inverted Nikon Eclipse Ti microscope stand, Trim-scope II scan-head into which is coupled IR pulsed excitation light from a Ti:Sapphire laser system and OPO. This risk assessment covers the daily, routine use of the microscope system as a whole. A laser specific risk assessment covers the laser / optical path maintenance.
Describe the Laser / There are two possible external laser sources available to the Trim-scope. 1) A Coherent Chameleon Ultra II Ti:Sapphirewhich is pumped by internal diodes. This laser can be automatically tuned to emit over a range of 680 – 1080 nm with no cavity adjustment required from the user. Output power is ~ 3.6 W at 830 nm and a variable amount (0 – 100%) of the output can be coupled directly into the scan-head. 2) An APE OPO PP840 cavity source which utilises ~80% of the output from the Chameleon Ti:Sapphire laser as a pump source. The OPO emits between 1100 – 1500 nm with maximum output power of 1080 mW at 1100 nm.
Describe the Beam Delivery System / All beams are enclosed in permanent beam tubes with mirror mounts and periscopes covered by boxes. Access is available to adjust beam steering mirror positions by removal of individual box lids.
Describe the Laser Process / IR light from the Ti:Sapphire and OPO laser enters the microscope body via the scan-head. It is reflected upwards by a dichroic and focussed to a point by an objective lens. The produced fluorescence is then collected by the same objective and detected using PMTs located at exit ports of the microscope.
Describe the Environment / The Ti:Sapphire power supply is located under the optical table. Ancillary equipment is stored on shelving above the table.
Who uses the product or could affect its operation? / All suitably trained users could use it.
What PPE is available and what are the markings? / When optimising the beam coupling in to the scan-head IR blocking goggles must be worn: LG9 Thorlabs amber goggles (720-1075 DM L5, 720-750 + >1064-1075 IR L5, 750-1064 IR L7).
To what part of the life cycle of the activity does this risk assessment apply? / Normal Operation,
YOU MUST HAVE A RISK ASSESSMENT WHICH COVERS THE MOST HAZARDOUS PART OF THE ACTIVITY, FOR EG ALIGNMENT AND CHANGE OF USE