Running 1St and 2Nd Dimension Protein Samples
IPG strips (acrylamide)
Solubilization Solution(see assessment no. 1988 for full details)
Acetone
70% EthanolHazardous Substances Policy - Assessment
CHEMICAL HAZARD AND RISK ASSESSMENT
School of Biosciences
Name of supervisor / Prof N Brown / Assessment Number* / 1989Signature
Assessor / Rita Godfrey / Date of Assessment / 21/08/02
Signature
NotesA School COSHH form in Word is available on the School Server.
Available from the Health and Safety Unit.
Guidance on making an assessment is given in Making a Chemical Hazard and Risk Assessment.
Guidance is also available from Guidance on Completing the Chemical Hazard and Risk Assessment Form.
Use a continuation sheet to expand any section of this form in hard copy version.
1 / LOCATION OF THE WORK ACTIVITY / T1012 / PERSONS WHO MAY BE AT RISK
List names where possible / Post Docs, Post Graduates and Technicians in T101
3 / ACTIVITY ASSESSED / Running 1st and 2nd Dimension Protein Samples
4 / MATERIALS INVOLVED / Attach copies of data sheet(s)
NAME / AMOUNT
max/stored / HAZARD / RISK PHRASES / HAZDAT NO*** / BIOSCIENCESNO***
IPG strips (acrylamide) / Hazard unknown but since they are acrylamide gel strips it would be wise to treat with caution – see acrylamide below
Solubilization Solution
(see assessment no. 1988 for full details) / 5ml / Category 3 Carcinogen
Dangerous for the environment / R40 Possible risk of irreversible effects
R63 Possible risk of harm to the unborn child
R51/53 Toxic to aquatic organisms, may cause long-term adverse effects in the aquatic environment
Acetone / 2.5l / Highly Flammable
Irritant / R11 Highly flammable
R36 Irritating to the eyes
Repeated exposure may cause skin dryness or cracking
Vapours may cause drowsiness and dizziness / 4
70% Ethanol / 200ml / Highly Flammable / R11 Highly Flammable / 84
Siliconising Solution / 200ml / Flammable
Irritant / R10 Flammable
R36 Irritating to the eyes
Separating/Stacking Gels
0.5M/1.5M Tris
10% SDS
Acrylamide (Protogel: 30% acrylamide:0.8% bisacrylamide)
Ammonium Persulphate
10% ammonium persulphate
TEMED / 250ml
100ml
1l
100g
10ml
50ml / NSH
NSH
Toxic
Category 2 Mutagen
Oxidising
Harmful
Irritant
Harmful
Highly Flammable
Corrosive
Harmful / R45 May cause cancer
R46 May cause heritable genetic damage
R8 Contact with combustible material may cause fire
R22 Harmful if swallowed
R36/37/38 Irritating to eyes, respiratory system and skin
R42/43 May cause sensitisation by inhalation and skin contact.
R42/43 May cause sensitisation by inhalation and skin contact
R11 Highly flammable
R34 Causes burns
R20/22 Harmful by inhalation and if swallowed / 307
1165
1176
5XSDS Running Buffer:
Tris
Glycine
SDS
5x SDS running buffer / 1kg
1kg
1kg
2l / Irritant
NSH
Harmful
NSH / R36/37/38 Irritating to the eyes, respiratory system and skin
R22 Harmful if swallowed
R36/38 Irritating to eyes and skin / 911
1169
966
5 / INTENDED USE**
Give brief details and attach protocol/instructions
Running of protein samples on 1st and 2nd dimension gels
6 / RISKS to HEALTH and SAFETY from INTENDED USE
From personal exposure or hazardous reactions. Refer to OELs, flash points, etc., as appropriate. Are pregnant women, breast-feeding mothers especially at risk?
There is a risk to health and safety from handling the IPG strips and the Protogel solution.
There is a fire risk from the acetone, ethanol, TEMED and the siliconizing solution.
There is a health risk from using the solubilizing solution and a risk to the aquatic environment from this also.
There is a risk of contact with Tris, SDS and ammonium persulphate during the procedure.
7 / CONCLUSIONS ABOUT RISKS
Is level of risk acceptable? Can risk be prevented or reduced by change of substance/procedure? Are control measures necessary?
The risks mentioned above can be controlled by using certain measures
8 / CONTROL MEASURES
Additional to Good Chemical Practice
Keep the ethanol, acetone, siliconising solution and TEMED away from sources of ignition.
Protective clothing and gloves must be worn when handling ammonium persulphate, acrylamide, the IPG strips and the solubilising solution. Bear in mind that vinyl and pvc gloves offer only limited protection against acrylamide. The ammonium persulphate should be weighed out preferably in a fume hood and failing that a dust mask should be worn.
Care should be taken when weighing out SDS and Tris so as not to generate dusts. Gloves should be worn when doing this.
The solubilising solution must not go down the sink.
9 / INSTRUCTION/TRAINING
Specify course(s) and/or special arrangements.
10 / MONITORING
Performance of control measures,
Check fume hood is working before using. Check integrity of gloves.
Personal exposure / Health Surveillance
Occupational Health must be notified before you commence this procedure due to the presence of a respiratory sensitizer and carcinogens. Tel: 42695
11 / WASTE DISPOSAL PROCEDURE
See School Server for Approved Procedure Document on specific Chemical Waste Disposal.
Any waste acrylamide should be polymerised before placing in a bag and then disposing in lab waste bin.
Waste ethanol, acetone, siliconising solution, solubilising solution and TEMED should be put into the Waste Non-Halogenated solvent bottle which is taken down to Stores for specialist disposal when full.
All the other solutions can be safely washed down the sink with plenty of running water.
12 / REVIEW
Enter the date or circumstances for review of assessment (maximum review interval 5 years)
August 2007
13 / EMERGENCY ACTION
TO CONTROL HAZARDS / To stabilize situation eg spread absorbant on liquid spill; eliminate sources of ignition, etc.
Turn off all sources of ignition. Spills of ethanol, acetone, TEMED, siliconising solution and solubilising solution should be mopped up with paper towels or covered in absorbant powder from the lab spill kit and if possible the liquid should be carefully transferred to a sealable container, labelled and taken to Stores for disposal.
Spills of acrylamide should be carefully collected into a container and polymerised before disposal in a bag
Ammonium persulphate should be carefully swept up avoiding generating a dust and placed in a sealable container, labelled and taken to Stores for disposal.
TO PROTECT PERSONNEL / Evacuation, protection for personnel involved in clean-up, Special First Aid
Protective clothing, gloves (not vinyl or pvc for use with acrylamide) and goggles or face mask should be used when dealing with any spillages of compounds listed in this assessment. Large spillages of solvents require the area to be evacuated. A dust mask must be worn when clearing up ammonium persulphate.
TO RENDER SITE OF EMERGENCY SAFE / Clean-up/decontamination
The area should be ventilated to remove all traces of flammable vapours. The site can be washed with detergent and plenty of water whilst wearing the protective measures mentioned above.
CONTACT PHONE
10.10.00
* Prefix T is used for Teaching Assessment Number.
** Please include amount of chemicals used and how.
***Hazdat No is the UNICOSHH datasheet report number.
Biosciences No is the Biosciences data sheet number.
UNICOSHH IS A CHEMICAL DATABASE ON THE HEALTH AND SAFETY UNIT SERVER.
BIOSCIENCES DATA SHEETS ARE AVAILABLE IN THE SCHOOL SAFETY OFFICE.