Remarkably Increased Water-Dispersibility of Zinc Phthalocyanine Nanowire and Its Application

Supplementary Information

Remarkably increased water-dispersibility of zinc phthalocyanine nanowire and its application in cancer phototherapy

Hye Kyung Moon, Minhyeok Son, Ji Eun Park, Seok Min Yoon, Sang Ho Lee*, Hee Cheul Choi*

FigureS1.ThermogravimetriccurverecordedfortheZnPcPWobtainedinAr atmosphere and heatingrateof5 oC/min.ThermalvaporizationofZnPcPWstartsat 550 oC.

FigureS2.FT-IRspectraofα-ZnPcNW(red)andβ-ZnPcPW(black).

FigureS3.XRDpatternofZnPcNWsgrownonaSi(100)substrate.

FigureS4.Massspectrumof(a)ZnPcNWand(b)ZnPcPW.

FigureS5.1HNMRspectraof(a)ZnPcNWand(b)ZnPcPW.

FigureS6.(a, b)Dark-fieldscanningTEM(STEM)imageswithcorrespondingEDX elementary (C,N,O,Zn)mappingofZnPcNWtruncatedstructuresafter sonication.

FigureS7.(a)FT-IRspectraofZnPcNW(red)andZnPcPW(black).ThebandofZnPc NW at 3600-3300cm-1iscorrespondingtotheO-Hstretchingvibrationofwatermolecule. (b) XPSsurveyspectraofZnPcNWexposedtoair(left)andvacuumannealed(right).The O1s peakobservedataround533eVisduetoadsorbedH2O.

FigureS8.UV-visabsorptionspectraforZnPcNWsolubilizedinDMFandwatermixture solvent atdifferentratios.Thepeakisblue-shiftedfrom343nmto330 nmaswatercontent increase. TheinsetimageisaphotoofZnPcNWsolutions withdifferentDMF/waterratios.

FigureS9.(a)FT-IRspectraofPcNW(red)andPcPW(black).TheO-Hstretchingband at 3600-3300cm-1appearsinPcNWbutnotinPcPW,whichissimilar behavior of ZnPc NW and PW.(b)UV-visspectrumofPcNW solution.Thespectrum shows well-resolved Soret and QbandlikeZnPcNWaqueous solution.Theinsetimageis a photo ofPcNWaqueous solution.

FigureS10. Dynamic light scattering (DLS) data of ZnPc NW and ZnPc PW. (a, b)Size distributions taken right after sonication for 40 min showing about 86 and 90 nm ofhydrodynamic sizes for ZnPc NW and ZnPc PW, respectively. (c,d) Size distributionstaken after 1 h of sonication. While the ZnPc NW still shows about 87 nm of hydrodynamic size, ZnPc PW shows a high polydispersity index (0.918) owing to severe aggregations.

FigureS11.The absorption change of ZnPc NW aqueous solution during irradiation with 660 nm (a) and 808 nm laser (b), respectively.

FigureS12.Representativehistologicimagesoftumortissuetreated with ZnPcNWand NIR irradiation. (a-d)Hematoxylinandeosin(HE)stainingoftumortissuetreatedwith ZnPc NW+NIR,untreated,ZnPcS4+NIR,andPBS+NIR,respectively.Mostpink-stained cells aredead(pink:cytoplasm,violet:nucleus).(e-h)Deoxynucleotidyltransferase biotin-dUTP nick-endlabeling(TUNEL)assayforthedetectionofspecificapoptoticcellsafter treating with ZnPcNW+NIR,untreated,ZnPcS4+NIR,andPBS+NIR,respectively. Apoptotic cells arestainedbrownandnon-apoptoticcellsarecounterstainedbluewith hematoxylin. Thescalebaris50µm.