RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES
KARNATAKA, BANGALORE
PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION IN
M.D (AYURVEDA)
A PHARMACEUTICO-ANALYTICAL STUDY OF ASHTAMANGALA GHRUTHA AND ITS EXPERIMENTAL EVALUATION W.S.R TO ITS ANTI -PYRETIC ACTIVITY
BY
DR.SHIMI BEN.C.J,
1st year PG Scholar ,
Dept.of P.G Studies in Bhaishajya Kalpana,
Alva’s Ayurveda Medical College, Moodbidri
Guide
DR.CHANNABASAPPA.M.BELAGAVI. MD (Ayu),
Asst.Professor,P.G.Deptarment of Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri, Karnataka. .
Co-Guide
DR. PRASHANTH. B.K. MD (Ayu), PhD,
Asst.Professor,P.G.Department of Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri,Karnataka.
2011-2012
ALVA’S AYURVEDA MEDICAL COLLEGE, MOODBIDRI
From
Dr.Shimi Ben C.J,
1st year P.G Scholar,
Dept. of P.G. studies in Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri – 574227
To
The Registrar,
Rajiv Gandhi University of Health Sciences,
Karnataka, Bangalore.
Through
The Principal,
Alva’s Ayurveda Medical College,
Moodbidri, D. K. District.
Sub: Submission of completed Proforma for registration of subject for Dissertation in M.D (Ayurveda)
Respected Sir,
I request you to register the below mentioned subject in my name for the submission of dissertation to Rajiv Gandhi University of Health Sciences, Karnataka, Bangalore, as the partial fulfillment of M.D (Ayu) in Bhaishajya Kalpana.
TITLE OF DISSERTATION:
A PHARMACEUTICO-ANALYTICAL STUDY OF ASHTAMANGALA GHRUTHA AND ITS EXPERIMENTAL EVALUATION W.S.R. TO ITS ANTIPYRETIC ACTIVITY
I am here with enclosing the completed proforma for registration of dissertation subject in M.D (Ayu).
Thanking you
Yours sincerely
Moodbidri,
Dr.Shimi Ben.C.J.
RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,
KARNATAKA, BANGALORE.
ANNEXURE II
PROFORMA FOR REGISTRATION OF SUBJECT FOR DISSERTATION
1 .Name of the candidate : DR.SHIMI BEN C.J,
Address (in block letters) IST YEAR P.G. SCHOLAR,
DEPT. OF P. G. STUDIES IN BHAISHAJYA
KALPANA,
ALVA’S AYURVEDA MEDICAL COLLEGE,
MOODBIDRI, D. K. DISTRICT,
KARNATAKA - 574227
Permanent address : D/O Dr.C.O.JANARDHANAN,
“PRASANTHI”,
CHENDAMANGALAM.P.O,
N.PARAVOOR,
ERANAKULAM DISTRICT,
KERALA - 683512
2. Name of the
instituition : ALVA’S AYURVEDA MEDICAL COLLEGE,
MOODBIDRI, MANGALORE TALUK,
D.K. DISTRICT,
KARNATAKA - 574227
3. Course of study : MD (AYU) IN BHAISHAJYA KALPANA
and subject
4. Date of admission
to the course : 13-08-2011
5. Title of the topic :
A PHARMACEUTICO - ANALYTICAL STUDY OF ASHTAMANGALA GHRUTHA AND ITS EXPERIMENTAL EVALUATION W.S.R TO ITS ANTI-PYRETIC ACTIVITY
6. BRIEF RESUME OF THE INTENDED WORK:
6.1Need for the study:
In the present era, in general practice fever is the most common symptom seen by the physicians. It has caused more concern to all human beings irrespective of age, sex, cast, creed, social status etc.
Jwara is the disease which is given prime importance in most of the classics. According to Acharya Charaka Santhapa is the cardinal feature of Jwara.1 Fever2 can be defined as an elevation of body temperature that exceeds normal daily variation and occurs in conjunction with an increase in the hypothalamic set point.
Even though, there are number of synthetic drugs in other systems of medicine, they are not devoid of undesirable side effects. There are different formulations which are mentioned in different classics. One among them is ‘Ashtamangala‘ghrutha3.
The ghrutha formulation has several advantages over other pharmaceutical preparations in terms of absorption,shelf life etc. Because of longer duration of preparation more dissolution of active constituents of drugs takes place and the potency of ghrutha is to be enhanced .The formulation selected for the study is ‘Ashtamangala ghrutha’ explained in Sahasrayoga,ghrutha prakarana as sarwajwarapaha. Hence it will be prepared according to classical reference and will be analysed physico -chemically and will be evaluated its anti-pyretic effect experimentally.
6.2 Review of Literature:
Review of literature includes thorough screening of classical literature regarding the disease and the drug. So it will be dealt under three headings.
1. Drug Review
The formulation Ashtamangala Ghrutha is taken from the classical text Sahasrayogam, ghrutha yoga prakarana , 9th sloka.
.
Description of ingredients:
Sanskrit name / Botanical name / Family / Part used / QuantityPatola / Tricosanthes dioica4
Roxb. / Cucurbitaceae / Root / 1 Part
Sariva / Hemidesmus indicus 5
R.Br. / Asclepiadaceae / Root / 1 Part
Mustha / Cyperus rotundus6 Linn. / Cyperaceae / Tubers / 1 Part
Madhuka / Glycerrhiza glabra7Linn. / Leguminosae / Root / 1 Part
Katukarohini / Pichrorhiza kurroa8
Royle ex.Benth. / Scrophularaceae / Rhizome / 1 Part
Usira / Vetivera zizanoides 9
(Linn).Nash. / Graminae / Root / 1 Part
Chandana / Santalum album 10Linn. / Santalaceae / Heartwood / 1 Part
Pippali / Piper longum11 Linn. / Piperaceae / Fruit / 1 Part
Jala(water) / 16 times of the Kalka
Moorchitha
Ghrita (Cow’s
ghee) / 4 times of the Kalka
2. Pharmaceutical review:
The pharmaceutical process involved in this study is Ghrutha kalpana. The classics will be screened for this purpose particularly Sharangadhara samhita12 Madhyama Khanda(9/9-16)and Sahasrayoga Ghrutha prakarana, 9th sloka, and Bhaishajya Ratnavali13(5/1275) for ghrutha moorchana process.
3. Disease review:
Jwara being a symptom in various conditions is explained in several classical literatures.
Thus the disease review will be collected from these sources. Contemporary texts, previous works and journals will also be reviewed and recorded.
Some of the reference books are:
Ø Charaka Samhita
Ø Madhava Nidana
Ø Bhaishajya Ratnavali
Ø Harrison’s Principle of Internal Medicine
6.3 Objectives of the study:
· To prepare Ashtamangala ghrutha as per classical ghrutha kalpana.
· To analyse Ashtamangala ghrutha physico-chemically.
· To evaluate the anti pyretic effect of Ashtamangala ghruthta experimentally.
6.4 Hypothesis
H0 : Ashtamangala ghrutha will not be having anti-pyretic activity on experimental rats.
H1 : Ashtamangala ghrutha will be having anti-pyretic activity on experimental rats.
7. MATERIALS AND METHODS:
7.1 Source of data
This being a pharmaceutical and experimental study the source of data will be dealt under three headings.
A: Pharmaceutical source:
The formulation selected for present study ‘Ashtamangala ghrutha’ will be prepared in the Laboratory of P.G Department of Bhaishajya Kalpana attached to Alva’s Ayurveda Medical college,Moodbidri as per textual references.
B: Analytical study:
The ghrutha will be subjected for following physico-chemical analysis-
Ø Loss on drying at 110oc
Ø Specific gravity
Ø Refractive index
Ø Viscosity
Ø Saponification value
Ø Unsaponifiable value
Ø Acid value
Ø Iodine value
Ø Ester value
Ø Free fatty acid
Ø Total fatty matter
Ø TLC etc
C: Experimental source:
Sample of ghrutha will be analysed for its antipyretic action. The study will be carried out in 18 healthy albino rats with 6 animals in each 3 groups.The experiments will be conducted on Albino rats of either sex that are maintained in standard laboratory, food, water and atmosphere in animal house.
7.2. Method of collection of data (including sampling procedure (if any):
(a)Sample: 18 Albino rats will be selected randomly and grouped into 3, each group comprising of 6 rats.
(b)Inclusion criteria: Healthy active Albino rats of either sex each weighing between 150- 200 g.
(c)Exclusion criteria:
Ø Pregnant rats
Ø Infected rats
Ø Rats weighing below 150g and above200g
Ø Rats which are under trial for other experiments
(d)Grouping:
For Antipyretic study - Brewer’s Yeast Inducing Pyrexia Method. 15
Each group contains 6 albino rats and will be kept in separate cages.
SI.no: / Grouping / No. of rats / Drugs1. / Control / 6 / Distilled water
2. / Standard / 6 / Paracetamol
suspension
3. / Trial / 6 / Ashtamangala ghrutha
(e)Dosage:
The standard dose for administration of the drugs will be calculated as per the table of
Paget and Barne’s.16 (Rat dose conversion formula)
Rat dose/kg body.wt= Human dose x Body surface area factor (0.018) x 5
(f) Duration of study: 1 day
(g)Route of administration: oral
(h)Statistical test: As per the data observed statistics will be worked out through appropriate tests.
7.3. Does the study require any investigations or interventions to be conducted on patients or other humans or animals? If so please describe briefly.
Yes, all the experiments should be conducted on Albino rats of either sex that are maintained on standard laboratory food,water and atmosphere in animal house.
7.4. Has ethical clearance has been obtained from your institution in case of 7.3?
Yes, clearance will be obtained through the ethical committee formulated in the institution.
8. REFERENCES
1. Acharya Agnivesha , Charaka Samhita ,Elaborated by Charaka and Drudhabala with Ayurveda Dipika commentary, By Chakrapani Dutta ,Edited by Acharya Yadavji Trikamji , Choukhambha Prakashan, Varanasi;Reprinted 2009. page no:199.
2. Tinsley. R.Harrison,Harrison’s Principles of Internal Medicine,Vol.1,Mc Graw-Hill Medical Publishing Division,16th edition,2005,page no:104.
3. Vaidyan Krishnan.K.V& Pillai Gopala .S.,Sahasrayogam,Vidyarambham publications,2007,page no: 320.
4. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia, reprint edition 2010 page no: 250.
5. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia , reprint edition 2010, page no: 390.
6. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia, reprint edition 2010, page no: 452.
7. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia, reprint edition 2010, page no: 152.
8. Shastry. J.L.N, Dravyaguna Vijnana,Chaukhamba Orientalia, reprint edition 2010, page no: 390.
9. Shastry. J.L.N, Dravyaguna Vijnana,Chaukhamba Orientalia, reprint edition 2010, page no: 558.
10. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia, reprint edition 2010, page no: 478.
11. Shastry. J.L.N, Dravyaguna Vijnana, Chaukhamba Orientalia, reprint edition 2010, page no: 452.
12.Sharangadhara,SharangadharaSamhitha,Adhamalla, Kasirama virachitha Gudarthadeepika, Hindi vyakhya .Chaukambha Orientalia,Varanasi,7th edition,Page 212
.
13.Das govinda, Bhaishajya Ratnavali, edited by Misra Brahma Shankara, Hindi commentary by Sastri Dutta Ambika,Chaukhambha Sanskrit Sansthan,Varanasi,2005,Page 185.
14. Madhavakara, Madhava Nidanam , English commentary, Moorthy Srikanta ,Chaukambha Orientalia,Varanasi,2009,Page6.
15. Gerard V.H, Wolfgang V.H.Chapter H-G,Antipyretic Activity in: Drug Discovery and Evaluation,Springer.Pharmacological assays.Pg-418-420
16.. Paget G.E and Barnes J.M,Evaluation of drug activities in Pharmacometrics,Edited by Laurence P.R. and Bacharach A.L, Newyork Academic Press,1964.Pg-161
9. Signature of the candidate :
10. Remarks of the guide :
11. Name of the
11.1 Guide : DR.CHANNABASAPPA.M.BELAGAVI.), MD (AYU),
Asst.Professor,Dept. of P.G studies in Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri, D.K district,
Karnataka - 574227
11.2 Signature of the guide :
11.3 Co-guide : DR.PRASHANTH.B.K. MD (Ayu), Ph D,
Asst.Professor,Dept.ofP.G studies in Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri, D.K district
Karnataka-574227
11.4 signature of the co-guide :
11.5 Head of the department : DR.M.S.KRISHNAMURTHY. MD (Ayu),Ph.D,
Asst.Professor,Dept. of P.G studies in Bhaishajya Kalpana,
Alva’s Ayurveda Medical College,
Moodbidri, D.K district,
Karnataka- 574227
11.6 Signature and seal :
12.1 Remarks of the Dean/
Principal :
12.2 Signature and seal :