A COMPARITIVE STUDY OF MICROWAVE ASSISTED EXTRACTION VERSUS SOXHLET EXTRACTION IN THE ANALYSIS OF IN VITRO ANTIOXIDANT STUDY OF MICHELIA CHAMPACA LINN FLOWERS

M.PHARM DISSERTATION PROTOCOL

SUBMITTED TO

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA.

BY

SHABNA V.

B.Pharm.

UNDER THE GUIDANCE OF

JAISHREE VAIJANATHAPPA

M.Pharm. Ph.D

DEPARTMENT OF PHARMACEUTICAL CHEMISTRY

SAC COLLEGE OF PHARMACY

B. G. NAGARA, KARNATAKA

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA

Curriculum Development Cell

CONFIRMATION FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

Registration No.

Name of the Candidate - SHABNA.V

Address -SAC COLLEGE OF PHARMACY

B.G. NAGARA-571448

NAGAMANGALA(TALUK)

MANDYA (DIST)

KARNATAKA

Name of the Institution - S.A.C. COLLEGE OF PHARMACY

B.G. NAGARA

Course of study and Subject - M.Pharm. In Pharmaceutical Chemistry

Date of admission to Course -04-06-2008

Title of the Topic - A comparative study of microwave assisted extraction

versus soxhlet extraction in the analysis of In vitro

antioxidant study of Michelia champaca Linn flowers.

Brief resume of the intended work - Attached

Signature of the Student -

Guide name - JAISHREE VAIJANATHAPPA

M.pharm. Ph.D

Remarks of the Guide - The candidate is working under my direct

supervision in laboratories of SAC College

of Pharmacy

Signature of the Guide -

HOD name - Dr. B. RAMESH

M.pharm. Ph.D.

Signature of the HOD

Principal name - Dr. B. RAMESH

M.pharm. Ph.D.

Principal mobile no. – 9845863779

Principal e-mail I.D. -

Remarks of principal

Principal signature -

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

BANGALORE, KARNATAKA

ANNEXURE-II

1 / Name of the candidate & Address / SHABNA.V
SAC COLLEGE OF PHARMACY
B.G. NAGARA- 571 448
NAGAMANGALA (TALUK)
MANDYA (DIST)
KARNATAKA
PERMANENT ADDRES
VENKITTATHODI HOUSE
PO: RANDATHANI
PIN:676510
DIST: MALAPPURAM
ST: KERALA
2 / Name of the institute / SAC COLLEGE OF PHARMACY
B.G. NAGARA
KARNATAKA -571448
3 / Course of the study / M.PHARM IN PHARMACEUTICAL CHEMISTRY
4 / Date of admission / 04-06-2008
5 /
Title of the topic
/ A COMPARITIVE STUDY OF MICROWAVE-ASSISTED EXTRACTION VERSUS SOXHLET EXTRACTION IN THE ANALYSIS OF IN VITRO ANTIOXIANT STUDY OF MICHELIA CHAMPACA LINN FLOWERS
06 /

BRIEF RESUME OF THE INTENDED WORK

Introduction
Michelia champaca Linn (Family: Magnoliaceae), Synonyms: champakam, champak, sampige, sempakam, campakam, campakah. It is an evergreen tree, native to South Asia and Southeast Asia. It is best known and cultivated for its strongly fragrant yellow or white flowers. The plant was traditionally used for fever, cough, leprosy and post partum protection, in childbirth and as febrifuge. Flowers and flower buds are bitter, digestive, carminative stomachic and antipyretic properties [1]. Its anti-inflammatory and antipyretic activities [2] were reported. The active phytoconstituents sesquiterpene lactones, parathenolide and costunolide [3-9] were isolated from the plant. So far no biological activities are reported on the flowers of Michelia champaca. Based on the above observation, we are interested to carry out phytochemical investigation and in vitro antioxidant studies of Michelia champaca flowers extracts.
6.1 Need for the study
It is widely distributed throughout India and abundance in Kerala. A few reports on this plant have been published so far. The flowers of the plant Michelia champaca is selected for the phytochemical and in vitro antioxidant study. Hence, we are interested to compare the microwave assisted extraction versus soxhlet extraction in the analysis of in vitro antioxidant properties, which can be used for further research.
6.2 Review of literature
M. R. Khan et al., (2002) reported antimicrobial activity of Michelia champaca.
Vimala. R, et al., (1997) reported anti-inflammatory and antipyretic activity of Michelia champaca
Sethi VK, et al., (1984) reported constituents of Michelia champaca and Lewis acid catalysed transformations of parthenolide in to guaianolides
Hoffmann JJ, et al., (1977) reported cytotoxic agents from Michelia champaca and Talauma ovata: parthenolide and costunolide
Banerjee SK.et al., (1964) reported Micheline A from Michelia champaca Linn.
Banarjee SK, et al., (1964) reported Iriodenine from Michelia champaca Linn
Ulla Jacobsson, et al., (1995) reported sesquiterpene lactones from Michelia Champaca Linn.
Jaishree V, et al., (2008) have reported the methods of in vitro antioxidant properties
07 /
6.3 Objective of study
1)  Authentification and collection of the flowers of Michelia Champaca Linn
2) Microwave assisted and soxhlet extraction of Michelia Champaca flowers.
3) In vitro antioxidant studies of microwave assisted and soxhlet extracts.
Materials and Methods
Preparation, methods and screening methods will be followed according to literature survey.
7.1  Source of data
. 1) Laboratory based studies
2). Journals and publications
3) www.sciencedirect.com
4) www.pubmed.com
5) www.google.com
7.2  Methods of collections of data
Same as above
7.2.1 Collection of plant material and extraction
The flowers will be collected from Malappuram, Kerala. The air-dried powdered flower will be subjected to microwave assisted and soxhlet extraction with different solvents. The extracts were dried and kept in freeze for further use.
7.2.2 In vitro antioxidant studies
The individual extracts will be subjected to preliminary phytochemical investigation and in vitro antioxidant studies of different extracts will be carried out.
7.3) Does the study requires any investigations or interventions to be conducted on patients or other humans or animals?

NO

7.4) Has ethical clearance been obtained from your institution in case of 7.3?
NOT APPLICABLE
8) /

References:

1) Orient Longman²Indian Medicinal Plants” Vol 4, p. 32-35.
2) Perry LM.Medicinal plants of east and Southeast Asia: attributed properties and uses. Cambridge, Massachusetts, and London: The MIT Press.1980. p.154
3) Vimala R, Nagarajan S, Alam M, SusanT, Joy S. Antiinflammatory and antipyretic activity of Michelia champaca. Indian J. Exp. Biol. 1997, 35, 1310-1314.
4) Khan MR, Kihara M, Omoloso AD. Antimicrobial activity of Michelia champaca. Fitoterapia 2002, 73, 744-748.
5) Sethi VK, Thappa RK, Dhar KL, Atal CK. Constituents of Michelia champaca and Lewis acid catalysed transformations of parathenolide in to guaianolides. Planta Med.1984, 50, 364.
6) Hoffmann JJ, TorranceSJ, Widehopf RM, Cole JR. Cytotoxic agents from Michelia champaca Linn and Talauma ovata: parthenolide and costunolide. J. Pharm. Sci. 1977, 66, 883-884.
7) Banerjee SK, Chakravarti RN. Micheline A from Michelia champaca Linn. Bull. Calcutta Sch Trop Med.1964, 12, 113.
8) Banerjee SK, Chakravartirn, Fales HM. Iriodenine from Michelia champaca. Bull. Calcutta Sch Trop Med.1964, 12, 23-24.
9) Ulla J, Vijaya Kumar, Shantini S. Sesquiterpene lactones from Michelia Champaca. Phytochemistry 1995, 39, 839-843.
10) Jaishree V, Badami S, Suresh B.In Vitro Antioxidant Activity of Enicostemma
axillare, 54 (2008) 524-528.
09 /

Signature of Candidate

/ SHABNA.V
10 /

Remarks of the guide

/ The candidate is working under my direct supervision in laboratories of SAC College Of Pharmacy,
11 / Name and Designation
(In block letters)
11.1 GUIDE
Guide ship reference No. of RGUHS
11.2 Signature
11.3 Head of the Department
11.4 Signature /

JAISHREEVAIJANATHAPPA

M.Pharm, Ph.D
Associate Professor
Department Of Pharmaceutical Chemistry,
S.A.C. College of Pharmacy,
B.G.Nagara- 571448
Nagamangala (Taluk)
Mandya (Dist)
Karnataka
Dr. B. RAMESH
M.Pharm, Ph.D
Department Of Pharmaceutical Chemistry,
S.A.C. College of Pharmacy,
B.G.Nagara-571 448
Nagamangala (taluk)
Mandya (dist)
Karnataka
12 / 12.1  Remarks of the Principal
12.2 Signature