Protocol #: Approval Date:

TNPRC Only

Projects: Assigned Vet: Census Code:

Institutional Animal Care & Use Committee

PROTOCOL for the Use of Live Nonhuman Vertebrates

(other than NHPs) in Research, Teaching or Demonstration

IACUC Office, 1430 Tulane Ave. TB 23 New Orleans, LA 70112 504-988-6868

TNPRC IACUC Office, 18703 Three Rivers Road, Covington La 70343 985-871-6636

Email a copy of this protocol to http://tulane.edu/asvpr/iacuc/index.cfm

Date Filed: Principal Investigator: Position/Title:

Division / Department: E-mail: Phone: Lab Phone:

Address/Mailbox:

Co-investigator and/or Tulane contact: (required if PI is an outside collaborator at the TNPRC)

Position/Title: Division / Department: E-mail:

Additional Contact Person(s): Phone: Email:

Emergency Contact: Phone:

TITLE OF PROTOCOL:

List the Title of the grant only if it differs from the protocol title:

TYPE OF REVIEW: FUNDING AGENCY OR SOURCE:

PROJECT START DATE: DURATION OF PROJECT: - Please include animal use for the duration of the entire study. While protocols are approved for three years the committee needs to review the complete project.

TNPRC ONLY

IS THIS PROTOCOL A SUBCONTRACT OR FEE-FOR-SERVICE?

NO

YES (If the PI of the grant is affiliated with an institution other than TNPRC, “yes” should be indicated.)

IF YES, INDICATE PI OF THE GRANT:

INITIAL PROTOCOL –Proceed to Section III. Species

RENEWAL PROTOCOLS – List protocol number: Start at Section I. A

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SECTION I. RENEWAL PROTOCOLS ONLY

Please enter a brief summary of what has been accomplished thus far so that the continuation efforts described in this protocol can be better understood. Please include any relevant publications from your study.

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SECTION II. ANIMALS CURRENTLY ASSIGNED (animals housed in the animal facility)

Animals are currently in the DCM animal facility. Please note that animals must be transferred when the renewal protocol is approved. Comment if needed:

SECTION III. SPECIES, NUMBER & CATEGORY OF ANIMAL USE

*http://tulane.edu/asvpr/iacuc/index.cfm for detailed description of each USDA category

SPECIES / STRAIN / NUMBER / *USDA CATEGORY
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
.MouseRatRabbitPigFerretGuinea PigCatXenopus FrogOther-use grey box / . BCDE
COMMENT:

IMPORTANT

All questions must be answered in order for the protocol to undergo IACUC review.

Do not refer to or attach passages from grants.

SECTION IV. REGULATORY INFORMATION

A. Please check YES or NO as it applies to the species requested. If you answer YES to a question, you must provide an explanation and justification.

1. Is this an endangered or threatened species?

No

Yes

2. Is shortterm (> 30 minutes; < 12 hours) physical restraint of a conscious animal employed?

No

Yes

3.  Is longterm (12 hours or more) physical restraint of a conscious animal employed?

No

Yes

4.  Will specialized management procedures be required, e.g. specialized caging equipment, restricted or altered feeding/watering/sanitization schedules, or environmental parameters such as cage size, temperature, lighting or other fall outside the “Guide for the Care and Use of Laboratory Animals”?

No

Yes

5.  Will you perform more than one major survival surgery on any animal? PLEASE NOTE: major survival surgery penetrates and exposes a body cavity or produces substantial impairment of physical or physiologic functions (such as laparotomy, thoracotomy, craniotomy, joint replacement or limb amputation).

No

Yes

6.  Will animals be housed outside of AAALAC-accredited Tulane facilities for over 12 consecutive hours? If YES, the proposed site must be inspected and approved by the IACUC prior to use. Please list the location with justification below.

No

Yes

I have read the Policy for Satellite Housing http://tulane.edu/asvpr/iacuc/hsc/sops.cfm and will provide applicable SOPs. The satellite housing must be approved by the IACUC prior to the start of the study.

7.  Will pain or distress without the use of analgesic or anesthetic agents occur during the course of experimentation? This is Category E and must be scientifically justified with references.

No

Yes

8.  Will these procedures be filmed, videotaped or photographed for use outside the institution? If so, you must obtain approval from the Executive Director of Comparative Medicine. (985-871-6285)

No

Yes

9.  Does this protocol include a breeding colony?

No

Yes - Please complete the Breeding Appendix located at http://tulane.edu/asvpr/iacuc/hsc/deadlines.cfm

SECTION V. PERSONNEL HAZARDS

A. Is there potential exposure of humans to chemical hazards, physical hazards, radioactive agents, or biohazardous agents during the course of animal experimentation?

No

Yes - please respond to the choices below.

1. Physical Indicate the hazard:

2. Chemical Indicate the hazard:

3. Radioactive Indicate the hazard:

4. Biohazard / Recombinant DNA Indicate the hazard:

Does this biohazard require IBC approval? http://tulane.edu/asvpr/biosafety/committee/index.cfm

Yes, please provide the IBC#

No

Personnel from the Office of Biosafety or the Office of Environmental Health and Safety may contact you for additional information or completion of forms.

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SECTION VI. NON-TECHNICAL SUMMARY

Using non technical language (lay) that a layperson with no scientific background would understand, provide a brief summary that describes the nature and purpose of the this study and its potential value to human or animal health the advancement of knowledge or the betterment of society. Do not use scientific jargon, acronyms or abbreviations and do not cut and paste from grant applications.
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SECTION VII. COMPLETE DESCRIPTION OF ANIMAL USE

A.  HYPOTHESES - Please state your hypotheses in this section but do not cut and paste passages from your grant application. It is recommended that you limit this to approximately 20 lines.

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B.  DESCRIPTION - Provide a concise description including all steps, in chronological order, the experimental course of an animal from its entry into the experiment to the endpoint of the study. It is recommended that you provide a table or tables, which includes the Animal Group Composition, Sampling, Procedures, Drugs, Compounds and Time points. Please provide a table title reference if using more than one table.


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Insert table®

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C.  STANDARD OPERATING PROCEDURES (SOPs)

Procedures may reference an approved TULANE SOP/Policy in lieu of a complete description. Please choose all of the SOPs that will be used on your study. SOP/policies listed here are available at http://tulane.edu/asvpr/iacuc/hsc/sops.cfm or contact the IACUC office.

If there is no approved SOP then a complete description must be included in VII.B.

.P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID / .P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID
.P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID / .P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID
.P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID / .P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID
.P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID / .P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID
.P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID / .P6.03 Satellite HousingP6.04 Transportation of Animals PolicyP6.05 Use of NPGs PolicyP6.07 Policy of Guillotine Maintenance P6.08 Policy of Humane Experimental Endpoints in RP6.10 Rodent ID Techniques3.5 CO2 euthanasia.doc3.6 Blood Collection Techniques5.2 Procedures for Transgene IDTNPRC 3.31 Rodent Transport DT to TNPRCTNPRC 3 45 Rodent BioaerosolTNPRC 3.46 Rabbit Blood Collection TNPRC 3.47 Mouse Blood Coll. TechniquesTNPRC 3.57 Euthanasia of Lab Animals Using CO2TNPRC 3 59 Transgene ID

D.  PROPOSED TREATMENTS

ALLEVIATION OF PAIN AND DISTRESS: Describe the use of analgesics, anesthetics, and tranquilizing drugs to minimize discomfort, distress, pain or injury. The list below contains some common drugs and doses and must be checked if they will be administered in this protocol.

MOUSE AND RAT
DRUG / DOSE / ROUTE / FREQUENCY
Preanesthetic
Atropine / 0.05 mg/kg / IM/SC / Prior to anesthesia
Anesthetic Drugs
Ketamine/Xylazine / 80-110/5-10 mg/kg / IM/IP / As needed
Pentobarbital / 50-80 mg/kg / IP / As needed
Isoflurane gas / 1-5% in oxygen, to effect / Inhalation / Continuous
Analgesic Drugs
Buprenorphine / 0.05-0.1 mg/kg / IM/SC/IP / BID-TID
Caroprofen / 1-2 mg/kg / SC/PO / BID
Meloxicam / 1-2 mg/kg / SC/PO / SID
Local anesthetics/analgesics (can be combined in same syringe)
Lidocaine / 0.5%, maximum dose 7 mg/kg / SC/intra-incisional / Fast onset, but short duration (1 hour)
Bupivicaine / 0.25%, maximum dose 8 mg/kg / SC/intra-incisional / Slow onset, but long duration (4-8 hours)
OTHER analgesics, anesthetics, and tranquilizing drugs that will be used:
RABBIT
DRUG / DOSE / ROUTE / FREQUENCY
Preanesthetic Drugs
Glycopyrrolate / 0.01 mg/kg / IM/SQ / Prior to anesthesia
Sedative Drugs
Acepromazine/Buprenorphine / 1/0.02 mg/kg / IM/SQ / As needed
Ketamine/Diazepam / 20-25/1-3 mg/kg / IM/SQ / As needed
Anesthetic Drugs
Isoflurane gas / 1-5% in oxygen, to effect / Inhalation / Continuous
Ketamine/Xylazine / 20-35/1-3 mg/kg / IM / As needed
Analgesic Drugs
Buprenorphine / 0.01-0.05 mg/kg / IM/SQ / BID
Meloxicam / 0.1-0.2 mg/kg / IM/PO / Once daily
OTHER analgesics, anesthetics, and tranquilizing drugs that will be used:
PIG
DRUG / DOSE / ROUTE / FREQUENCY
Preanesthetic
Atropine / 0.04 mg/kg / IM/SC / Prior to anesthesia
Anesthetic Drugs
Tiletamine-Zolazepam/Xylazine / 4-6/2-3 mg/kg / IM/IV / As needed
Pentobarbital / 40-50 mg/kg / IV / As needed
Isoflurane gas / 1-5% in oxygen, to effect / Inhalation / Continuous
Analgesic Drugs
Buprenorphine / 0.05-0.1 mg/kg / IM / BID
Local anesthetics/analgesics (can be mixed and combined in same syringe)
Lidocaine / 0.5%, maximum dose 7 mg/kg / SC/IT/intra-incisional / Fast onset, but short duration (1 hour)
Bupivicaine / 0.25%, maximum dose 8 mg/kg / SC/IT/intra-incisional / Slow onset, but long duration (4-8 hours)
OTHER analgesics, anesthetics, and tranquilizing drugs that will be used:

List all drugs, agents, compounds, and substances that are introduced to the animals throughout the study period as described in the complete description.

SELECT TYPE / NAME / DOSE / ROUTE
.OTHER DRUGSVIRAL/BACTERIAL AGENTSRECOMBINANT VECTORSOTHER SUBSTANCES
.OTHER DRUGSVIRAL/BACTERIAL AGENTSRECOMBINANT VECTORSOTHER SUBSTANCES
.OTHER DRUGSVIRAL/BACTERIAL AGENTSRECOMBINANT VECTORSOTHER SUBSTANCES
.OTHER DRUGSVIRAL/BACTERIAL AGENTSRECOMBINANT VECTORSOTHER SUBSTANCES
.OTHER DRUGSVIRAL/BACTERIAL AGENTSRECOMBINANT VECTORSOTHER SUBSTANCES
IN VIVO RADIONUCLIDE(s) / VOLUME
INSECT VECTORS / NUMBER / MODE

E. VETERINARY CARE
Routine care as specified in the standard operating procedures

Ø  Division of Veterinary Medicine (985-871-6266) http://www.tnprc.tulane.edu/index.shtml