1 / Name Of The Candidate And Address
(In Block Letters) / DR ANUPAMA DESAI
POST GRADUATE STUDENT.
DEPARTMENT OF PERIODONTICS. COORG INSTITUTE OF DENTAL SCIENCES,
VIRAJPET.
2 / Name of the Institution / COORG INSTITUTE OF DENTAL SCIENCES.
3 / Course of study and Subject / MASTER OF DENTAL SURGERY, PERIODONTICS
4 / Date of admission
of Course / 21st APRIL 2007
5 / Title of the Topic
ESTIMATION AND COMPARISON OF SERUM CORTISOL LEVELS AND ACADEMIC PSYCHOLOGICAL STRESS AND THEIR EFFECT ON PERIODONTAL HEALTH.

RajivGandhiUniversityof Health Sciences, Karnataka

Bangalore

ANNEXURE II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DESSERTATION

6 / Brief Resume of the intended work
6.1Need for the study:
Periodontaldiseases are infections associated with microorganisms that colonise the subgingival area. However as with many chronic infections, the onset and progression of periodontal diseases are clearly modified by local and systemic host conditions or risk factors that markedly affect the resistance of host to infecting periodontal organisms.
Stress is a state of physiological or psychological strain caused by adverse stimuli, physical, mental or emotional, internal or external, that tends to disturb the functioning of an organism and which an organism Naturally tends to avoid. Over recent years several authors have discussed that psychological stress might be a risk factor for periodontitis.
Cortisol is the primary stress harmone. It is the major natural glucocorticoid in humans. Its levels in serum are raised during stressful conditions and serve as important marker in evaluating stress in human.
Very few authors have studied the role of stress as a possible psychological risk factor for periodontitis in students appearing for the academic exams. This study is done to evaluate the effect of stress on periodontal health.
6.2REVIEW OF LITERATURE
  1. Renate deizer, Stefan rutterman et al (1998) supported the hypothesis that psychological stress is a significant risk factor for periodontal inflammation.
  2. Lakshmi Byopati and Hom-Leywang (2000) suggested that stress is associated with more severe periodontal disease as well as poor healing response to traditional periodontal therapy.
  3. Renate deizer , Danniel Hilpert et al (2000) stated that psychological stress may induce neglect of oral hygiene and increase in plaque accumulation
  4. J B Hilgert, F N Hugo et al (2006) evaluated the extent severity of chronic periodontitis and its association with levels of salivary Cortisol. They suggested that Cortisol levels were positively associated with the extent and severity of periodontitis

7 / 6.3Objectiveof study:
To assess the psychological stress levelsin the subjects appearing for major academic exam and subjects who are not appearing for academic exam
Estimation of serum Cortisol levels in the subjects appearing for major academic exam and subjects who are not appearing for academic exam
To correlate and compare the stress,Cortisol levels on periodontal Health.
Materials And Methods:
7.1 Source of data:
The study is conducted on students 18 to 25 years of age, Coorg Institute of Dental sciences Virajpet.
7.2Method of collection of data:
Method
1) 40 students of age group 18 to 25 years are to be taken for the study.
2) Prior to the conductionof thestudy thorough oral hygiene instructions which includes standardized brushing technique is advised to all students.
3) periodontal status are evaluated 6 months and one weeks prior to the academic exam
4)serum Cortisol levels are measured 6 months and one weeks prior to the academic exam
Clinical parameters to be used:
Periodontal health is assessed by using
- Turesky – Gilmore – Glickman Modification of Quigley Hein plaque
index1970,
-gingival index (Loe and SilnesH, 1963)
-oral hygiene index (John C Greene and Jack R Vermilion 1960)
-Russels Periodontal Index (1956)
-serum Cortisol levels are assessed by using Electro Chemiluminence
immuno essay
-Student is asked to answer questionnaire at each time to evaluate the level of stress by using standardized stress questionnaire given by ( depression anxiety stress scale 42 by Lovibond and Lovibond (1995)
-Comparison of student’s periodontal status with psychological stress and serum Cortisol levels is done to know if there is any effect of stress on periodontal health.
Criteria for selection of subjects.
Inclusion Criteria.
Students of age group 18 to 25 years of age appearing for major academic exams
Exclusion Criteria
  • Subjects with systemic disease
  • Subjects on immunosuppressive disease
  • Subjects on immunosuppressive drugs.
  • Subjects who chronically use corticosteroids
  • Subjects who use tobacco
  • Subjects with psychological disorders
  • Subjects who have undergone oral prophylaxis for the past six months
  • Subjects on antibiotic therapy for the past six months
STATISTICAL ANALYSIS is done by using
Descriptive analysis
Product moment co relation
Paired sample t test
Repeated measure anova
7.3Does the study require any investigations or interventions to be conducted on the patients or other humans or animals? ( If so please describe briefly )
Yes, student’s 3ml of blood is collected to assess serum Cortisol level after taking Student’s written consent.
7.4Has Ethical clearance been obtained from your institution in case of 7.3?
Yes
8. References:
  1. Robert J. Genko et al 1998. Models to evaluate the role of stress in periodontal disease. Annals of periodontology 3(1):288-299
  2. Deinzer R et al. 2001. Effects of academic stress on oral hygiene – a potential link between stress and plaque associated disease? J Clin periodontol 28:459-464
  3. J B Hilgert et al 2006. Stress, Cortisol and periodontitis in a population aged 50 years and over. Dent Res 85:324-328
  4. Daiane C. Peruzzo et al 2007. Systematic review of stress and psychological factors as possible risk factors for periodontal disease. 78(8):1491-1504
  5. Lakshmi Boyapati, Hom-Lay Wang 2007. The role of stress in periodontal disease and wound healing. Periodontology 2000. 44:195-210