PROCEDURE 3-6:Microhematocrit Determination

Name______Date______Time______

PROCEDURE 3-6:Microhematocrit Determination

Equipment/Supplies: Microcollection tubes, sealing clay, microhematocrit centrifuge, microhematocrit readingdevice, hand disinfectant, surface disinfectant, gloves, biohazard container, sharps container

Standards: Given the needed equipment and a place to work, the student will perform this skill with _____ % accuracy in a total of _____ minutes. (Your instructor will tell you what the percentage and time limits will be before you begin.)

Key: 4 = Satisfactory0 = UnsatisfactoryNA = This step is not counted

Procedure Steps / self / PARTNER / INSTRUCTOR
1. Washed your hands; put on personal protective equipment /  /  / 
2. Assembled the equipment and supplies /  /  / 
3. Drew blood into the capillary tube by one of two methods:
a. Directly from a capillary puncture (see Chapter 41) in which the tip of the capillary tube is touched to the blood at the wound and allowed to fill to three-quarters of the way full or to the indicated mark
b. From a well-mixed EDTA tube of whole blood; again, the tip is touched to the blood and allowed to fill three-quarters of the tube /  /  / 
4. Place the forefinger over the top of the tube, wiped excess blood off the sides, and pushed the bottom into the sealing clay /  /  / 
5. Drew a second specimen in the same manner /  /  / 
6. Placed the tubes, clay-sealed end out, in the radial grooves of the microhematocrit centrifuge opposite each other; put the lid on the grooved area and tightened it by turning the knob clockwise (Note: Some microhematocrit centrifuges have the scale printed in the machine at the radial grooves.); closed the centrifuge lid; spun it for 5 minutes or as directed by the centrifuge manufacturer /  /  / 
7. Removed the tubes from the centrifuge and read the results (instructions are printed on the device; results should be within 5% of each other); took the average and reported resultsas a percentage /  /  / 
8. Disposed of the microhematocrit tubes in a biohazard container; properly cared for or disposed of other equipment and supplies; cleaned the work area; remove your gloves and washed your hands /  /  / 

Calculation

Total Possible Points: _____

Total Points Earned: _____ Multiplied by 100 = _____

Divided by Total Possible Points = _____%

PassFail



Student’s signature ______Date ______

Partner’s signature ______Date ______

Instructor’s signature ______Date ______