Preparation of Compounds for Use in Animals

This form describes the methods for diluting pharmaceutical grade compounds or preparing non-pharmaceutical compounds for use in animals. The IACUC must review and approve the use of all compounds before they are used. For more information, see IACUC Policy 210 and the instructions on the preparation of compounds for use in animals in IACUC Guidance 211.

Standard Operating Procedures

Preparation of Compounds for Use in Animals

Text in red is either an example or an instruction. Please add, delete or modify, as appropriate.

Principal investigator: / Protocol number(s):
Name of compound: / Species administered:
Route of administration: / Dose(s) administered:
Ingredient* / Purpose / Vendor and catalog number / Chemical grade / Hazardous
5-bromo-2’-deoxy-uridine (BrdU) / Active ingredient / Sigma B9285 / 99% pure / Yes
0.9% (w/v) saline / Diluent / Western Medical / USP / No

* There is no need to amend the protocol for changes in the vendor of USP or pharmaceutical grade ingredients. Changes to non-pharmaceutical grade compounds must be first reviewed and approved by the IACUC.

Preparation:

Describe the method of preparation, including the amounts of each ingredient (recipe). Note that diluents and inactive ingredients must also be USP grade, where possible.

Sterilization:

Include any details about how the compound will be sterilized, if appropriate. If the compound is not sterilized, indicate “Compound not sterilized.”

Testing:

Include any details about how the compound will be tested before being used in animals, if appropriate. If no testing for purity, efficacy, pyrogenicity, sterility, etc… will be performed, indicate “No testing is performed.”

Labeling:

The compound will be labeled with the following:

·  The name and concentration of all the ingredients in the compound

·  The preparation date

·  The use-by-date, which should not extend past the earliest expiration date of any of the components

Storage:

The compound will be stored <add details of the storage method>.

References:

Include any references about use of the non-pharmaceutical compound in similar studies, if available.

Include any references about storage methods and the stability of the compounds, if available.

If no references are available, indicate “No references available.”

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