Pre-lab 4f: Sterile Technique and Pouring Plates--CLASS SET
Directions: Answer the following questions ON YOUR OWN PAPER and in COMPLETE SENTENCES. Get the questions stamped by your teacher upon completion.
1. When is sterile technique used?
2. How do we sterilize media used in biotechnology?
3. Why is high temperature important for sterility?
4. What is sterile technique? (list examples of what you would do)
5. What materials are needed for this lab?
6. What should be done BEFORE you begin pouring plates?
7. We will not be using a flow hood so what should we do to reduce contamination?
8. Now read through the procedure for pouring the LB agar plates. Construct a flow chart (rough draft) that captures all steps in the procedure.
9. Once you are happy with your flow chart, add it to your laboratory notebook (you can cut and paste it in or re-draw it—it must be in black ink!).
10. Name 5 things you can do to decrease the chance of contaminating a sample.
11. When pouring plates, you notice that the agar is coming out in lumps. Why is this undesirable and what corrective measures can you take?
12. LB agar plates are needed for several experiments. If eight sleeves of Petri dishes (20 plates/ sleeve) are needed, each poured with about 20 mL, what total volume of LB agar should you prepare?
13. Read Lab 4g (bacterial cell cultures)—we will answer questions later.
Pre-lab 4f: Sterile Technique and Pouring Plates--CLASS SET
Directions: Answer the following questions ON YOUR OWN PAPER and in COMPLETE SENTENCES. Get the questions stamped by your teacher upon completion.
1. When is sterile technique used?
2. How do we sterilize media used in biotechnology?
3. Why is high temperature important for sterility?
4. What is sterile technique? (list examples of what you would do)
5. What materials are needed for this lab?
6. What should be done BEFORE you begin pouring plates?
7. We will not be using a flow hood so what should we do to reduce contamination?
8. Now read through the procedure for pouring the LB agar plates. Construct a flow chart (rough draft) that captures all steps in the procedure.
9. Once you are happy with your flow chart, add it to your laboratory notebook (you can cut and paste it in or re-draw it—it must be in black ink!).
10. Name 5 things you can do to decrease the chance of contaminating a sample.
11. When pouring plates, you notice that the agar is coming out in lumps. Why is this undesirable and what corrective measures can you take?
12. LB agar plates are needed for several experiments. If eight sleeves of Petri dishes (20 plates/ sleeve) are needed, each poured with about 20 mL, what total volume of LB agar should you prepare?
13. Read Lab 4g (bacterial cell cultures)—we will answer questions later.