pmT-Sapphire-CytERM-17 Sequence Number: CERM-10x
ATGGTGAGC - mT-Sapphire Sequence (717 Nucleotides - 239 Amino Acids)
TCCTACGGC - mT-Sapphire Chromophore (SYG); A206K = AAG
ATGGATCCT - Rabbit Cytochrome p450 Sequence (87 Nucleotides - 29 Amino Acids)
LINKER - 17 Amino Acids (51 Nucleotides) between mT-Sapphire and CytERM
ATG - Methionine Start Codon
TAG TAA TGA - Stop Codons Antibiotic = Kan/Neo
GTTTAGTGAACCGTCAGATCCGCTAGCGCTACCGGACTCAGATCTGGTACCCTTGCAGCTTCCAGTTGTCAGGAAGAGAAGGCTCCCATGGATCCTGTGGTGGTGCTGGGGCTCTGTCTCTCCTGTTTGCTTCTCCTTTCACTCTGGAAACAGAGCTATGGGGGAGGGAAGCTTCGAATTCTGCAGTCGACGGTACCGCGGGCCCGGGATCCACCGGTCGCCACCATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCTCCTACGGCGTGATGGTGTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTTCAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCATCCAGTCCAAGCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTGGGCATGGACGAGCTGTACAAGTAAGCGGGCCGCGACTCTAGATCATAATCAGCCATACCACATTTGTAGAGGGTTTTACTTGCTTTAAAAAACCTCCCACACCTCCCCCTGACTGAAACATAAATGAATGCATTGTGTGTACTT
mT-Sapphire = EGFP + L64F-T65S-Q69M-C70V-S72A-Y145F-V163A-S175G-T203I-A206K
mT-Sapphire-CytERM-17 in HeLa Cells