Online Resource 1.GenBankaccessionnumbersforthesequencesused in thephylogeneticanalyses. Specimenssequencedforthisstudyaremarkedwith an asterisk (*). A dash (–) indicatesmissingdata.

Aethionemeae: Aethionemasaxatile(L.)W.T.Aiton, GQ284853, Q288726; Alysseae: AlyssumlinifoliumStephan exWilld., KF512500, KF022888; Galitzkyapotaninii(Maxim.) V.Bocz., EF514656, KF022983; Anchonieae: Anchoniumbillardierei, DQ357513,–;Anchoniumelichrysifolium,DQ357514, –; Aphragmeae: AphragmuseschscholtzianusAndrz., DQ165334, –; A. oxycarpus(Hook.f. & Thomson) Jafri, DQ165337, –;Arabideae: ArabisalpinaL., DQ060111, DQ288731;Aubrietadeltoidea(L.)DC.,KF547215, DQ288734; Asteae: Asta schaffneri(S.Watson) O.E.Schulz, HQ541168, –; A. strictaRollins, HQ541172, –; Biscutelleae:BiscutellaauriculataL., DQ452057, –;BiscutellacichoriifoliaLoisel., KF022693, –;BiscutelladidymaL., KF850615, –;BiscutellalaevigataL., DQ452056, KF023014; HeldreichiabupleurifoliaBoiss. subsp. bupleurifolia, FN397975, KX987128*;HeldreichiabupleurifoliaBoiss. subsp. rotundifolia (Boiss.) Parolly, NordtMumm. var.rotundifolia,FN397982, KX987129andKX987130*; H. bupleurifoliasubsp. rotundifoliavar.atalayii(Kit Tan) Parolly, NordtMumm., –, KX987131*; H. bupleurifoliasubsp. bourgai(Boiss.) Parolly, NordtMumm.,FN397973, –; H. bupleurifoliasubsp. polymorphaParolly,Nordt, Eren & Mumm., FN397972,–;LunariaannuaL., KF022719, KF023035;LunariaredivivaL.,GQ424543, –;MegadeniapygmaeaMaxim., GQ424544, KX987132*; Ricotiaaucheri(Boiss.) Burtt,KR262072, –; R. carnosulaBoiss. & Heldr.,KR262080, KX987133*;R. creticaBoiss. & Heldr.,KR262091, –; R. davisianaBurtt, KR262078, –;R. isatoides(Barley) Burtt, KR262074, –;R. lunaria(L.) DC.,KR262103, –;R. sinuataBoiss. & Heldr.,KR262101, –;R. tenuifoliaSibth. & Sm.,KR262076, –;R. variansBurtt, KR262104, KX987134*.Bivonaeeae: BivonaealuteaDC.,HQ327490, –;Brassiceae: BrassicaoleraceaL., EF601912,DQ288742;Hirschfeldiaincana(L.) Lagr.-Foss.,AY722470,DQ288778; Calepineae: Calepinairregularis(Asso) Thell., AY722504, –; Goldbachialaevigata(M.Bieb.) DC.,DQ357545, DQ288771; Cardamineae:Cardaminebulbosa(Schreb. exMuhl.)Britton, SternsPoggenb.,EU819316,HM120272;CardaminehirsutaL.,EU819351,HM120271;Cochlearieae: Ionopsidiumacaule(Desf.) Rchb.,HQ268659, DQ288785;I.megalospermum(Maire) M.A.Koch, HQ268630,–; Coluteocarpeae: Noccaeacaerulescens(J.PreslC.Presl) F.K.Mey.,KT220625, DQ288806; Noccaeathlaspidioides(Pall.) F.K.Mey.,DQ249838,DQ288804;Conringieae: Conringiaperfoliata(C.A.Mey.) N.A.Busch, AY722505, –; ConringiapersicaBoiss., –, DQ288756; Zuvandaexacoides(DC.)Askerova, DQ357607, –; Cremolobeae: CremolobussubscandensKuntze,–, DQ288757;MenonvilleahookeriRollins,JX470565,DQ288797; MenonvilleapinnatifidaBarnéoud,KF662738, –;Euclidieae: Euclidiumsyriacum(L.)W.T.Aiton,–, DQ288767; Strigosellaafricana(L.)Botsch.,–,DQ288793;Eudemeae:BrayopsiscolombianaAl-Shahbaz, KM376244, EU718525; EudemanubigenaHumb. & Bonpl.,EU620297, EU718545; Eutremeae: Eutreamaaltaicum(C.A.Mey.) Al-ShehbazWarwick, DQ165365, DQ288836; E. heterophyllum(W.W.Sm.) H.Hara, DQ165352, DQ288768; Heliophileae: Heliophilaafricana(L.) Marais, South Africa, Western Cape, Cape Town, K. Mummenhoff 1002 (OSBU), AJ863602/AJ864808, –; H. coronopifoliaL., DQ249846, –;Heliophila sp., –, DQ288775; Hesperideae: HesperismatronalisL., DQ288776, –;Hesperisrechingeri, KF023027, –;Iberideae: IberisamaraL., AJ440311, –; IberissempervirensL.,–, DQ288781; I. spathulataBergeret, AJ440312, –; IberisumbellataL.,–,HM120281;Isatideae: IsatistinctoriaL.,GQ131323, DQ288786;MyagrumperfoliatumL.,GQ424547,DQ288800;Kernereae: Kernerasaxatilis(L.) Sweet,AJ440314, –; RhizobotryaalpinaTausch, AJ440315, –; Lepidieae: LepidiumalyssoidesA.Gray,KF022714, DQ288789; LepidiumdrabaL.,KJ623488, DQ288790;Megacarpaeeae: Pugioniumcornutum(L.) Gaertn., JF978166, FJ183791; P. dolabratumMaxim., JF978171, –;Notothlaspideae: NotothlaspiaustraleHook.f., AF100689, –; N. rosulatumHook.f., AF100690, –; Schizopetaleae: MathewsiafoliosaHook. & Arn.,DQ357563, EU718555; SchizopetalonbiseriatumPhil., KC174394, EU718565; Scoliaxoneae: Scoliaxonmexicanus(S.Watson) Payson, HQ541175, –;Sisymbrieae: SisymbriumaltissimumL., KJ557141, DQ288826; Sisymbriumlinifolium(Nutt.)Nutt.,–,DQ288821; S. septulatumDC., KJ557137, –;Stevenieae: MacropodiumpterospermumF.Schmidt, GU182055, –; Pseudoturritisturrita(L.) Al-Shehbaz, GU592179, –; Thelypodieae: Chaunanthuspetiolatus(Hemsl.) O.E.Schulz, EU620289, –;Stanleyapinnata(Pursh)Britton, EU620319, DQ288832;StreptanthuscrassicaulisTorr.,–, DQ288750;Thlaspideae: Alliariapetiolata(M.Bieb. )CavaraGrande,KJ748666, DQ288727; ThlaspiarvenseL.,KT220620,DQ288839.