OIE RL for West Nile Encephalitis Federica Monaco Italy

OIE RL for « West Nile Encephalitis » – « Federica Monaco » – « Italy »

OIE Reference Laboratory Reports
Activities in 2012

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

Each ToR(blueitalicised text)has been placed as a heading covering the group of questions related to it.

Please note the reditalicisedtext is given as guidance and should be deleted from your report and substitute with your data. Examples are based onpast Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

Plaque Reduction neutralization test (PRNT)

Cell Culture (Vero)

Real-time RT-PCRWNV lineage 1

Real-time RT-PCRWNV lineage 2

Real-time RT-PCRWNV lineages 1 and 2

IF (Flavivirus Group)

Genome sequencing

Mosquitoes morphological identification

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

Tostore and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagents officially recognised by the OIE or other international bodies?

X Yes No

3.Did your laboratory supply standard reference reagents to OIE Member Countries?

X YesNo

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

X Yes No

5.Did your laboratory produce vaccines?

YesX No

6.Did your laboratory supply vaccines to OIE Member Countries?

YesX No

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

X YesNo

8.Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

YesX No

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

X Yes No

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

X Yes No

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

X Yes No

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Did your Laboratory collect epizootiological data relevant to international disease control?

X Yes No

13.Did your laboratory disseminate epizootiological data that had been processed and analysed?

X Yes No

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a) Articles published in peer-reviewed journals:...... 5

b) International conferences:...... 7

c) National conferences:...... 2

d) Other:...... internet

• For publications see annex: “List of publications”

ToR: To provide scientific and technical training for personnel from OIE Member Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

X Yes No

a) Technical visits:......

b) Seminars:......

c) Hands-on training courses:...... 2

d) Internships (>1 month):......

ToR:To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned

16.Does your laboratory have a Quality Management System certified according to an International Standard?

X Yes No

17.Is your laboratory accredited by an international accreditation body?

X Yes No

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

X Yes No

ToR:To organise and participate in scientific meetings on behalf of the OIE

19.Did your laboratory organise scientific meetings on behalf of the OIE?

X Yes No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

X Yes* No

*The table content refers to the participation in international meetings

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

X Yes No

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

X Yes No

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

X Yes No

ToR:To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results.

24.Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

X Yes No

ToR:To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

X Yes No

Annex : List of publications

List of publications:

1. Savini G., Capelli G., Monaco F., Polci A., Russo F., Di Gennaro A., Marini V., Teodori L., Montarsi F., Pinoni C., Pisciella M., Terregino C., Marangon S., Capua I., Lelli R. 2012. Evidence of West Nile virus lineage 2 circulation in Northern Italy. Vet Microbiol. doi:10.1016/j.vetmic.2012.02.018.
2. Mulatti P., Capelli G., Lorenzetto M., Terregino C., Monaco F., Bonfanti L., Marangon S. Surveillance of West Nile Virus in north-eastern Italy, 2011: equine IgM and mosquitoes to early detect virus circulation. Zoonoses and Public Health. 2012 Sep 13. doi: 10.1111/zph.12013.
3. Lelli R, Calistri P, Bruno R, Monaco F, Savini G, Di Sabatino D, Corsi I, Pascucci I. West Nile transmission in resident birds in Italy. Transbound Emerg Dis. 2012 Oct; 59 (5):421-8. doi: 10.1111/j.1865-1682.2011.01287.x. Epub 2011 Dec 28.
4. Barbić L., Listeš E., Katić S., Stevanović V., Madić J., Starešina V., Šeparović S., Di Gennaro A., Savini. G. Spreading of West Nile virus infection in Croatia. Vet Microbiol. 2012 Oct 12;159(3-4):504-8. doi: 10.1016/j.vetmic.2012.04.038. Epub 2012 May 9.
5. Di Francesco G., Savini G., Maggi A., Cavaliere N., D’Angelo A. R., Marruchella G. 2012. Equine meningo-encephalitis caused by Halicephalobus gingivalis: a case report observed during West Nile disease surveillance activities. Veterinaria Italiana 48: 431-443.

International conferences:

1. Goffredo M. “Training on Identification of Culicoides and Mosquitoes at Species Level and Molecular Typing of Culicoides Species”, TAIEX 46424 Ankara, Turkey, 17 - 18 April 2012.
2. Savini G., Monaco F., Briguglio P., Pinto S., Pinoni C., Polci A., Di Gennaro A., Pais M., Teodori L., Bruno R., Ruiu A., Lelli R. West Nile disease in Sardinian wild birds. 6th Annual Meeting EPIZONE Brighton, UK, 12 - 14 June 2012.
3. Fares F., Savini G., Goffredo M., Cipriani M., Di Gennaro A., Marini V., Santilli A., Ippoliti C., Monaco F., Lelli R. West Nile and Usutu virus vectors in Italy. 6th Annual Meeting EPIZONE, "Viruses on the move", Brighton, UK, 12 - 14 June 2012.
4. Guercio A., Savini G., Monaco F., Calistri P., Bruno, S. Di Bella, P. Di Marco, V. Di Marco, A. Torina, G. Purpari, R. Lelli. West Nile Disease (WND) in Sicily. 15th International Congress of Infectious Diseases, Bangkok, Thailand, 13 - 16 June 2012.
5. Purpari G., Savini G., Monaco F., Calistri P., Di Gennaro A., Cannella V ., Vitale F., Mira F., Di Bella C., Guercio A., Lelli R. Importance of dogs as sentinels of West Nile virus activity in urban and suburban areas. 15th International Congress of Infectious Diseases, Bangkok, Thailand, 13 - 16 June 2012.
6. Savini G., Monaco F., El Harrak M., Sghaier S., Erturk A., Kennerman E., Calistri P., Lelli R. West Nile Disease in the Mediterranean Basin: activities and results of collaboration between the Istituto G. Caporale Teramo (Italy) and Middle East and North African countries. “Endemic and Emerging Infectious Diseases of Priority in the Middle East and North Africa: Research Opportunities and Biosafety in a Changing Environment”. Istanbul, Turkey, 18 - 21 June 2012.
7. Lelli R. Current West Nile Disease Status and Research. “Chronic Wasting Disease”. Seoul, South Korea, 18 - 23 June 2012.
8. Cito F., Narcisi V., Danzetta M.L., Iannetti S., Di Sabatino D., Bruno R., Carvelli A., Atzeni M., Sauro F., Calistri P. Analysis of surveillance systems in place in European Mediterranean countries for West Nile virus (WNV) and Rift Valley fever (RVF). Arbo-Zoonet final meeting, Joint Conference on Emerging and Re-emerging epidemics affecting global health. Orvieto, Italia, 19 - 21 September 2012.
9. Calistri P., Iannetti S., Danzetta M.L., Narcisi V, Cito F., Di Sabatino D., Bruno R., Sauro F., Atzeni M., Carvelli A., Giovannini A. The Components of “One World – One Health” Approach. Arbo-Zoonet final meeting, Joint Conference on Emerging and Re-emerging epidemics affecting global health. Orvieto, Italia, 19 - 21 September 2012.
10. Danzetta M.L., Iannetti S., Di Sabatino D., Bruno R., Cioci D., Cito F., Narcisi V., Primavera A. , Carvelli A., Atzeni M., Sauro F., Calistri P. An integrated information system for the management of West Nile Disease surveillance in Italy. Arbo-Zoonet final meeting, Joint Conference on Emerging and Re-emerging epidemics affecting global health. Orvieto, Italia, 19 - 21 September 2012.
11. Savini G. West Nile virus in Italy, five years of epidemic. Symposium “Epidemiological and clinical aspects of West Nile virus infection in Croatia and neighbouring countries” Zagreb, Croatia, 25 October 2012 (oral presentation).

National conferences:

1. Marruchella G., Di Francesco G., Vita S., Portanti O., Marini V., d'Angelo A.R., Savini G Infezione da virus west nile: protocolli immunoistochimici a confronto nel modello murino. XIV Congresso Nazionale della S.I.Di.L.V. Sorrento, 24 - 26 October 2012.
2. Savini G., Puggioni G., Di Gennaro A., Rossi R., Di Francesco G., Rocchigiani A., Polci A., Marini V., Pinoni C., Arru D., Rolesu S., Lorusso A., Monaco F. Ceppi del virus della West Nile appartenenti al lineage 2 isolati in uccelli selvatici in Sardegna. XIV Congresso Nazionale della S.I.Di.L.V. Sorrento, 24 - 26 October 2012 (oral presentation).
3. Di Francesco G., Savini G., Maggi A., Cavaliere N., D’Angelo A. R., Marruchella G. Meningo-encefalite equina da Halicephalobus gingivalis: contributo casistico nell’ambito del piano di sorveglianza West Nile disease. IX Congresso Nazionale dell’Associazione Nazionale di Patologia Veterinaria, Perugia, 25-26 May 2012.

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