OIE RL for «Chronic Wasting Disease» – « Hyun-JooSohn» – «Korea (Rep. of) »

OIE Reference Laboratory Reports
Activities in 2012

Name of disease (or topic) for which you are a designated OIE Reference Laboratory: / Chronic Wasting Disease
Address of laboratory / Chronic Wasting Disease laboratory
Animal Plant and Fisheries Quarantine & Inspection Agency (QIA), Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF)
175 Anyang-ro, Manan-gu, Anyang, Gyeonggi, 430-757, Republic of Korea
Tel.: / (82) 31-467-1867
Fax: / (82) 31-467-1830
e-mail address: /
website: /
Name (including Title) of Head of Laboratory (Responsible Official): / Dr. Yong Ho ParkDVM, MS, PhD
QIA Commissioner
Name(including Title and Position) of OIE Reference Expert: / Dr. Hyun-JooSohnDVM, MS, PhD
Chief of Chronic Wasting Disease laboratory
Date of submission to the OIE

Instructions

This form should be used by an OIE Reference Laboratory to report activities that took place from January through December of the past year (2012), unless otherwise stated, and must be submitted by the end of January every year.

Only those activities that concern the disease (or topic) for which the laboratory is recognised by the OIE should be mentioned. The questionnaire structure follows the Terms of Reference (ToRs) for OIE Reference Laboratories, available at:

Each ToR(blue italicised text)has been placed as a heading covering the group of questions related to it.

Please note the red italicised text is given as guidance and should be deleted from your report and substitute with your data. Examples are based on past Annual Reports or have been invented.

The questionnaire represents a means of gathering information on activities carried out by OIE Reference Laboratories and making it available to OIE Member Countries and to the OIE Reference Laboratory network.

This annual report will remain available for consultation on the OIE web site:

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ToR:To use, promote and disseminate diagnostic methods validated according to OIE Standards

ELISA tests for BSE are also conducted in local veterinary provincial lab

Disease / Test recommended by the OIE / Total number of test performed last year
CWD / diagnostic tests / Nationally / Internationally
ELISA (Bio-Rad) / 258 tests
Western blotting(WB) / 98 tests
Immunohistochemistry(IHC) / 98 tests
BSE / diagnostic tests / Nationally / Internationally
ELISA (Bio-Rad, IDEXX) / 18,845 tests
Western blotting(WB) / 133 tests
Immunohistochemistry(IHC)0 / 133 tests
Scrapie / Direct diagnostic tests / Nationally / Internationally
ELISA (Bio0Rad) / 68tests

ToR:To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards.

To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease.

2.Did your laboratory produce or store imported standard reference reagentsofficially recognised by the OIE or other international bodies?

Yes No

3.Did your laboratory supply standard reference reagentsto OIE Member Countries?

Yes No

Type of reagent available / Related diagnostic test / Produced/
stored / Amount supplied nationally
(ml, mg) / Amount supplied internationally
(ml, mg) / Name of recipient OIE Member Countries and of institutions
Control CWD paraffineembeded block / IHC / Produced / 20ea / 1ea / Taiwan AHRI,
Antibodies / IHC, WB / produced / 5 ml / 1ml / Taiwan AHRI,

4.Did your laboratory produce diagnostic reagents other than the OIE-approved standard reference reagents?

Yes No

5.Did your laboratory produce vaccines?

Yes No

6.Did your laboratory supply vaccines to OIE Member Countries?

Yes No

ToR:To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases

7.Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

Yes No

8.Didyour laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease?

Yes No

Name of the new test or diagnostic method or vaccine developed / Description and References (Publication, website, etc.)
Antibodies /
  1. Monoclonal antibody for discriminating and detecting an abnormal prion causing chronic wasting disease (2012. patent registration No. 10-1149496 from the industrial property office in Korea)
  2. Monoclonal antibody for discriminating and detecting an abnormal prion causing bovine spongiform encephalopathy (2012. patent registration No. 10-1149396 from the industrial property office in Korea)

ToR:To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries

9.Did your laboratory carry out diagnostic testing for other OIE Member Countries?

Yes No

10.Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country?

Yes No

Name of the OIE Member Country receiving a technical consultancy / Purpose / How the advice was provided
Malaysia regional veterinary Laboratory service / Interpretation ofPrPimmunostained slides for TSE / remote assistance

ToR:To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations

11.Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own?

Yes No

Title of the study / Duration / Purpose of the study / Partners (Institutions) / OIE Member Countries involved other than your country
Interspecies transmission of CWD / 5years / Transgenic mouse model studies include sika deer inoculation studies / Canadian food Inspection Agency / Canada

ToR:To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases

12.Didyour Laboratory collect epizootiologicaldata relevant to international disease control?

Yes No

13.Didyour laboratory disseminate epizootiologicaldata that had been processed and analysed?

Yes No

14.What method of dissemination of information is most often used by your laboratory?

(Indicate in the appropriate box the number by category)

a)Articles published in peer-reviewed journals:...... 4

b)International conferences:...... 3

c)National conferences:...... 8

d)Other:...... 0

a. Scientific publications in peer-reviewed journals

  • Kim HJ, Tark DS, Lee YH, Kim MJ, Lee WY, Cho IS, Sohn HJ, T Yokoyama. Establishment of a cell line persistently infected with chronic wasting disease prions. Journal of VeterinaryMedicine Science. 74(10):1377- 1380, 2012.
  • Lee YH, Sohn HJ, Kim MJ, Kim HJ, Lee WY, Choi YP, Tark DS, Joo IS, Kweon CH, Cho IS.PrPSc detection in affected tissues and blood of mice infected with murine-adapted Bovine Spongiform Encephalopathy strain 301C. Korean Journal of Veterinary public Health , 36(2) :81-88, 2012.
  • Kim HJ, Lee WY, Kim MJ, Tark DS, Cho IS, Sohn HJ, Lee YH. Gene expression profile of a persistently chronic wasting disease (CWD) prion-infected RK13 cell line. Korean Journal ofVeterinary public Health , 36(4) :81-88, 2012
  • Lee YH, Kim MJ, Tark DS, Sohn HJ, Yun EI, Cho IS, Choi YP, Kim CL, Kweon CH, Joo IS, Chung GS, Lee JH. Bovine SpongiformEncephalopathy Surveillance in Republic of Korea 31(3): 2012
  1. Presentations at international conferences and meetings
  • Kim HJ, Tark DS, Lee YH, Kim MJ, Lee WY, Cho IS, Sohn HJ,. Establishment of a cell line persistently infected with chronic wasting disease prions,International Conference on prion disease-Neuroprion 9-10 May 2012, Amsterdam Netherland
  • Lee YH, Sohn HJ, Kim MJ, Kim HJ, Lee WY, Yun EI, Tark DS, Cho IS. Strain characterization of the Korean CWD cases in 2001 and 2004. ,International Conference on prion disease-Neuroprion 9-10 May 2012, Amsterdam Netherland
  • Sohn HJ, Lee YH, Kim MJ, Kim HJ, Lee WY, Cho IS, Invited speaker “Current CWD research topics in ROK” Asian Pacific Prion Symposium, July 29-30 2012 Yokohama Japan
  1. Presentations at national conferences and meetings
  • Sohn HJ “An Overview of TSE diagnostics and research in RO Korea”The 1st QIA Workshop and Hands-on Training on CWD and Other Prion Diseases, in collaboration with the CFIA Canada and the NIAH, Japan(Anyang, Republic of Korea, 19-22 June 2012)
  • Sohn HJ, Lee YH, Kim MJ, Kim HJ, Lee WY, Park HC, Cho IS, The Current situation of Chronic Wasting Disease
  • Lee YH, Sohn HJ, Kim MJ, Park KJ, Kim HJ, Lee WY, Yoon EI, Cho IS, Biochemical and pathological characterization of CWD in VM, The Korean Society of Veterinary Science 1~2 Nov 2012
  • Kim HJ, Lee WY, Kim MJ, Tark DS, Cho IS, Sohn HJ, Lee YH, Gene expression profile of a persistently chronic wasting disease(CWD) prion-infected RK13 Cell Line, The Korean Society of Veterinary Science 1~2 Nov 2012
  • Lee WY, Kim HY, Kim MJ, Bang MH, Sohn HJ, Lee YH, Baek YS, Tark DS, Cho IS anti-prion activity of two natural products in persistently BSE prion infected M2B cell line, The Korean Society of Veterinary Science 1~2 Nov 2012
  • Sohn HJ, Polymorphism of the prion protein gene in cervid , The Korean Society of Veterinary Science 1~2 Nov 2012
  • Park KJ, Sohn HJ Lee YH, Kim MJ, Cho IS, Advancement of an extracting method of PrPSc from natural , The Korean Society of Veterinary Science 1~2 Nov 2012
  • Sohn HJ “An Overview of TSE diagnostics and research in RO Korea” 2012 Workshop on Diagnosis of Animal Diseases for Asian Countries Anyang, Republic of Korea, 23 Oct -2 Nov 2012

15.Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries?

Yes No

a)Technical visits:......

b)Seminars:...... 3

c)Hands-on training courses:...... 23

d)Internships (>1 month):......

Type of technical training provided (a, b,c or d) / Country of origin of the expert(s) provided with training / No. participants from the corresponding country
b, c / Taiwan / 1
b, c / Malaysia / 1
b, c / Philipinese / 1
c / Cambodia / 2
c / Indonesia / 2
c / Lao People’s Democratic Republic / 2
c / Malaysia / 2
c / Mongolia / 2
c / Myanmmar / 2
c / Nepal / 1
c / Philippines / 2
c / Singapore / 1
c / Thailand / 2
c / Vietnam / 2

16.Does your laboratory have a Quality Management System certified according to an International Standard?

Yes No

17.Is your laboratory accredited by an international accreditation body?

Yes No

Test for which your laboratory is accredited / Accreditation body
Immunohistochemistry / AHVLA

18.Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter 1.1.3 orManual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1)

Yes No

19.Did your laboratory organise scientific meetings on behalf of the OIE?

Yes No

20.Did your laboratory participate in scientific meetings on behalf of the OIE?

Yes No

ToR:To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results

21.Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

Yes No

22.Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests?

Yes No

23.Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest?

Yes No

Title of the project or contact / Scope / Name(s) of relevant OIE Reference Laboratories
Interspecies transmission of CWD / Transgenic mouse model studies include sika deer inoculation studies / OIE RL for CWD,Canada

24.Did your laboratory organiseor participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease?

Yes No

ToR: To place expert consultants at the disposal of the OIE

25.Did your laboratory place expert consultants at the disposal of the OIE?

Yes No

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Annual reports of OIE Reference Laboratories, 20121