NanoDrop user guide
1- Turn on the computer.
2- Launch the NanoDrop 2000 software.
3- Select the right assay (Nucleic Acid).
4- Wavelength verification step. Remove the kimtech under the arm of the nandrop and click OK.
5- Select the right type of sample (DNA, RNA, ssDNA, …) (colored top right field) and the absorption coefficient will be modified accordingly.
6- Before any measurement, ensure that the box “add to report” (top left) is checked in order to save the totality of your data.
7- Load 1.5 – 2 mL of ddH2O or DEPC H2O on the pedestal reading surface. Put the NanoDrop arm GENTLY down and click on “Blank” (top left).
8- Wipe the drop from the pedestal and arm surfaces with a clean kimtech.
9- Enter your sample ID.
10- Load 1.5 – 2 mL of your sample on the pedestal reading surface. Put the NanoDrop arm GENTLY down and click on “Measure” (top left).
11- Repeat steps 8 to 10 (cleaning, loading and reading) for all your samples.
12- Load 1.5 – 2 mL of ddH2O or DEPC H2O on the pedestal reading surface. Put the NanoDrop arm GENTLY down and read its absorbance as a sample by clicking on “Measure” (top left). You expect to have a 0ng/mL concentration and no absorbance peak. This step is to confirm the validity of your measurements.
13- Wipe the drop from the pedestal and arm surfaces with a clean kimtech and clean the NanoDrop using a kimtech moistened with ddH2O or DEPC H2O.
14- Click on the “report” bar (bottom left).
15- Click on the “Export” button (top left).
16- Save your data in a *.XML format file on a USB storage device.