More Introductory Material from “Medicine Quest”

Plotkin Lecture NCSTA 2000 Fall Greensboro

Natural Products Chemistry: “ The Poison is the dose”

Poisons have Antiviral Activity

I. “Fungus among us” Cordyceps fungus reated to the toadstool Fungus grows on exoskeleton  goes inside and devours nonvital organs part of brain

 Live at tree top fungus eats the insect

 spread lots of spores- this is source of cyclosporin which is an immunosuppresant which allows surgery especially organ transplants.

“Can you believe this organism is referred to as a lowly organism?”

II. Tropical Insects:

N1 811 does suppress immune system or antibodies and AIDS  blocks reproduction

This makes nature more important than before  final unique molecules

3 x 109 ways to make molecules: Western Medicines need cures for depression, schizophrenia and other mental illness.

III. Crab Spider eats Bee: must kill or be stung

Nervous system: Na, K membrane transport. Spider venom prevents transport therefore death to victim of bite.

IV. Scorpions: More important than Pandas, Rhinoceros and elephants.

Protect the species (of scorpion) because they are there.

Venom- helped to map human nervous system. Many classes of drugs were used.

V. Gila Monsters: Molecule that controls level of sugar in blood

Diabetes kills more than 4 times more people than Breast Cancer

VI. African Vipers: anticoagulant

Merck aggristatin

Coagulation is a 32 step cascade multi-step process

Cost $1x109 per year to care for diabetics.

VII. Copperheads in the Carolinas:

Not much anticoagulants .

Have a compound that interferes with antigens and tumors that send out fibers -this cpd stops growth ie stop Breast Cancer tumors

VIII. Rainforest: 22 years

Spices make food better

Mace: more important to kill bacteria

Spice Trade: “Origin of democracy”

Magii: Frankensense and Muir, Incense, goodies

Muir = “Gift of Life”, strong antibiotic properties

IX. Serendipity: Government gives Award for most wasteful funding.

Proxmire Award was given for

Firefly research: gene for luciferase used to see if genes are turned on

X. TB: is drug resistant

The gene that codes for luciferase is used to test the TB bacteria when combined with an antibiotic:

If light appears, it is a drug resistant strain i.e. TB will grow.

Biological Threat: Drug resistant bacteria swap plasmids

Suriname: found new genus of plant

Catsclaw: in health food stores in NC

Actually lichen on Catsclaw- contains alkaloids

Good or bad effects can happen

Need to know which species

XI. Cone Snails: kill by shooting out poison

Proboscis in paintings by Rembrandt

Kill human scuba divers

-This drug can be effective pain killer

-it is an opiate derivative (opiates are addictive, have confusing side effects)

-This poison has none of the opiate drawbacks

  1. Tanzania: collect daisies and swallow them whole

Monkeys’ Medicine Chest- new antibiotics inside in small sacks of leaves, if swollow whole sacks don’t open leaves have intestinal parasite

We are a planet of Beatles

Somari

Rhinoceroos

Smiley face

Dusthead

Frogs: large source of Biologically Active Molecules

Poison dart blow dart or skin

Abbott Labs in Illinois

New painkiller: different from morphine or cone snail

Green Frog: Green Monkey Frog “The Amazons”

Cut hands, rub frogs on cuts to heal

-heartbeat goes through the roof- lost bodily functions-woke 6 hours later felt like a god for 2 days

Use for Blood Pressure possibly

Peptids  new high blood pressure medicine

“No Matter how weird”  could be therapeutic

luciferase, FRFMide

Anticoagulants

Leeches: suck blood RP pilhrin

New anticoagulant based on leaches

Vampire Bats: suck blood

New anticoagulants

“Every Useful Plant learned of from local people”

Shaman Genius:

Combine plants smoke and dances with spiritual

A lot to learn

Bush Negro from N.E. Amazon

Escape to the Interior

True warriors never defeated

Maroons- 5 different tribes

Each write different pharmacopica

-get sickle cell anemia and treat it

Brazil: Shaman killed many

His son-in-law “you will not live long enough to regret argument”

Found dead in hammock

Tested tissues  found nothing

*How to ask questions*

What happens now?

Magic, Black Magic, Dosage

Healing Earache, Dandruff, Aching Back, Diabetes

Ex: Diabetes Blood Sugar 500----B6 in 12 hours

3 days/week------now go for 1 month

“How to Bring Them Together”

Problem: ‘The Culture is Dying, Disappearing faster than the Rain Forests”

“AmazonTeam.org”

Shaman’s Apprentice program with Harvard Medical School

N.W. Amazon, South Central Amazon, Brazil, Suriname Medicine Men usually resent each other

“Amazon Conservation Team” .org

Amazon Websites

nice picture of monkeys and Indians

highest biodiversity

“conservation.org”

“ACT”

amazonteam.aa.psiweb.com/focus/index.html

accessexcellence.org/RC/enthobotany/page3.html/page4

“Medicinal Plants”

Cinchona, Coca, Curare, pilocarpine, and pineapple

accessexcellence.org/TSN/S/chromatography_background.html

Tales of Shaman’s Apprentice Overview

  1. Through the Emerald Door

“The tropical rain forest s the greatest expression of life on earth”

Thomas Lovejoy 1983

II. Professor Richard Evans Schultes died week of April 6 2001

Father of ethnobotany: how people in region use plants

-foods, religion, hallucinogen, poisons

-photos of Indians hunting, fishing, and shamans

-2000 plants for medicine

III. The Amazon

-Home to the largest eagle, snake, anteater, armadillo, spider, freshwater turtle, and freshwater fish

-Toad that weighs 7lbs

-Rodent that weighs 120lbs, Capy Bara

-1/4 ton catfish

-1 in every 4 plants on earth in Amazon

-Many plants have chemicals toxic to insects, why affect humans differently- nutritious, poison, hallucinogenic, or theraputic

IV. Most Human Powerful Rxns to plant alkalods: 1N and 2C

-Stimulants: caffeine

-Addictive: cocain, heroin, and nicotine

-Poison: strychnine

-Analgesics: Codeine, morphine

-Hallucinogens: meseolin, and psilocybin

-Alkaline- bitter- Quinone (most important of 30)- cure malasia (more deaths than any other disease)- bark of cinchona tree (30 alkaloids)- Southern American Indians, Peru and Ecuador

-Wonder drugs waiting to be found

-Bugs can develop resistance to synthetics but not usually natural ones

-Ginko: 280 million years ago for asthma or allergic Rxns

-Taxol from Pacific Yew Tree: orarian cancer- Indians used for veneral disease arthritis

-Since 1900: 90 tribes extinct in Brazil- Rain Forest disappears at 100 acres/minute

-By 2000, 10% of plants extinct

-Shaman lore gone

V. Albert Hoffmann:

-Inventor of LSD

-Isolated psilocybin

-Cardiac beta blocker

VI. Rosy Periwinkle

- 70 alkaloids

- 6 have antitumor properties

- vinblastine: soaked whole plant in alcohol

- injected into rats; decreased WBC

VII. Eli Lilly:

- annual 100 millions

-Not one penny back to Madagascar

VII. Pasteur

-“Chance favors the prepared mind.”

Chapter 2 “Search for the Black Caiman” –30 ft long

First trip July 1979

Four eye fish

Epiphytes-airplants

Cuckoo relative with claws on wing

Black Caimon- nutrient concentrators for plankton

Longest mammal: tapir .25 tons

Spices:

Volatile oils

Spices: allsice, chili pepper, cardamon, cinnamon, cloves, nutmeg, mace, turmerie, vanilla, paprika

Seed Dispersal: 3 pass through to germinate

Plants evolve chem. defenses to bugs and insects

-Dev medicine

-Latex rubber trees

-Other imp. chem.. use

-Waxes, fibers

Chapter 3 “Among the Maroons”

-Suriname: cultural mosaic

-NE shoulder of S. America