Manuscript submitted to EcotoxicologyF Pomati & L Nizzetto

Supplementary online material

Supplementary online material

“Assessing triclosan-induced ecological and trans-generational effects in natural phytoplankton communities: a trait-based field method”

Francesco Pomati and Luca Nizzetto

Includes:

-Table S1. Average trait levels for Cytobuoy-derived functional groups.

-Table S2. Coefficients for linear fit functions applied to haldane rates.

-Table S3. P-values for linear fit functions applied to haldane rates.

-Figure S1. Photos of laboratory and field experimental setups.

-Figure S2. Half-life of triclosan in CE-membrane bags.

-Figure S3. Price equation partitioning of changes in total Chl-a FL.

Supplementary table S1 Average trait levels for each Cytobuoy-derived functional group. For information on Cytobuoy parameters (traits) refer to the main text. Number of cells by SWS = number of humps in the signal, proportional to number of cells for colonial phytoplankton.

Trait / GR1 / GR2 / GR3 / GR4 / GR5 / GR6 / GR7 / GR8 / GR9 / GR10 / GR11 / GR12
Length.SWS / 99 / 13 / 11 / 10 / 8 / 21 / 15 / 13 / 14 / 48 / 27 / 17
Total.FWS / 61214 / 2365 / 2205 / 1981 / 1232 / 5793 / 2329 / 3276 / 3095 / 16068 / 13327 / 6087
Total.SWS / 220287 / 2112 / 1475 / 1180 / 555 / 8968 / 2457 / 2729 / 3714 / 39590 / 30825 / 9243
Total.FL.Orange / 394 / 18 / 15 / 14 / 11 / 35 / 23 / 18 / 19 / 96 / 62 / 29
Total.FL.Red / 95246 / 4456 / 5221 / 4208 / 2691 / 8974 / 4528 / 9080 / 7496 / 19873 / 42894 / 21721
Total.2.FL.Red / 94989 / 2606 / 2691 / 2408 / 1595 / 6648 / 3689 / 4303 / 4276 / 17374 / 20822 / 9623
Total.FL.Yellow / 243 / 18 / 13 / 15 / 11 / 30 / 22 / 18 / 30 / 74 / 42 / 25
FillFactor.FL.Orange / 0.68 / 0.92 / 0.93 / 0.94 / 0.92 / 0.91 / 0.93 / 0.93 / 0.93 / 0.89 / 0.85 / 0.91
FillFactor.FL.Red / 0.45 / 0.64 / 0.66 / 0.69 / 0.79 / 0.56 / 0.73 / 0.59 / 0.64 / 0.46 / 0.51 / 0.55
FillFactor.2.FL.Red / 0.64 / 0.81 / 0.81 / 0.81 / 0.86 / 0.77 / 0.85 / 0.76 / 0.77 / 0.72 / 0.71 / 0.75
FillFactor.FL.Yellow / 0.83 / 0.95 / 0.96 / 0.94 / 0.93 / 0.96 / 0.95 / 0.95 / 0.72 / 0.91 / 0.90 / 0.96
NumberOfCells.SWS / 2.66 / 1.10 / 1.08 / 1.07 / 1.11 / 1.28 / 1.40 / 1.10 / 1.12 / 2.11 / 1.31 / 1.13
SWS/FWS / 4.14 / 0.94 / 0.71 / 0.64 / 0.56 / 1.56 / 1.37 / 0.88 / 1.10 / 2.40 / 2.41 / 1.56
Biovolume / 0.20 / 0.05 / 0.04 / 0.04 / 0.03 / 0.07 / 0.04 / 0.05 / 0.05 / 0.11 / 0.10 / 0.07

Supplementary table S2Coefficients for linear fit functions applied to haldane indexes of phenotypic evolution for selected traits (Fig. 4in the main text).

Traits / GR1 / GR2 / GR3 / GR4 / GR5 / GR6 / GR7 / GR8 / GR9 / GR10 / GR11 / GR12
Fill Factor FL Phyco / 0.268 / -0.090 / 0.003 / 0.065 / 0.073 / 0.172 / -0.064 / 0.063 / 0.280 / 0.056 / -0.025 / -0.039
Total FL Phyco / -0.343 / -0.054 / -0.061 / -0.082 / -0.035 / 0.018 / -0.074 / -0.016 / -0.012 / 0.082 / 0.074 / -0.084
Total SWS / -0.210 / -0.091 / -0.092 / -0.051 / -0.151 / 0.003 / 0.201 / -0.118 / -0.150 / -0.027 / -0.024 / -0.016
Length SWS / 0.616 / -0.039 / 0.056 / -0.048 / 0.107 / 0.047 / -0.102 / 0.056 / 0.011 / 0.002 / -0.003 / 0.050
Biovolume / 0.122 / -0.076 / -0.063 / -0.095 / -0.089 / 0.005 / -0.053 / -0.014 / 0.095 / 0.015 / 0.084 / -0.073
Total FL Chl-a / 0.159 / -0.028 / -0.007 / 0.067 / 0.009 / 0.266 / 0.037 / 0.117 / -0.116 / -0.019 / 0.105 / 0.223

Supplementary table S3 P-values for linear fit functions applied to haldane indexes of phenotypic evolution for selected traits (Fig. 4in the main text).

Traits / GR1 / GR2 / GR3 / GR4 / GR5 / GR6 / GR7 / GR8 / GR9 / GR10 / GR11 / GR12
Fill Factor FL Phyco / 0.113 / 0.411 / 0.968 / 0.386 / 0.496 / 0.029 / 0.655 / 0.227 / 0.305 / 0.404 / 0.748 / 0.126
Total FL Phyco / 0.027 / 0.230 / 0.204 / 0.156 / 0.418 / 0.676 / 0.482 / 0.635 / 0.916 / 0.553 / 0.291 / 0.060
Total SWS / 0.030 / 0.167 / 0.258 / 0.374 / 0.022 / 0.968 / 0.019 / 0.171 / 0.376 / 0.588 / 0.445 / 0.710
Length SWS / 0.048 / 0.724 / 0.442 / 0.040 / 0.262 / 0.433 / 0.462 / 0.114 / 0.958 / 0.973 / 0.973 / 0.298
Biovolume / 0.040 / 0.376 / 0.192 / 0.260 / 0.408 / 0.875 / 0.521 / 0.587 / 0.469 / 0.772 / 0.314 / 0.157
Total FL Chl-a / 0.046 / 0.720 / 0.881 / 0.258 / 0.840 / 0.000 / 0.733 / 0.005 / 0.248 / 0.599 / 0.050 / 0.001

Supplementary Fig. S1

Figure S1A-B: laboratory tests to assess the effects of membranes on the growth of phytoplankton communities. C-D: mesocosms experiments using dialysis bags (LN in the photo, taken by FP).

Supplementary Fig. S2

Figure S2Disappearance of triclosan in CE-membrane bags during laboratory tests (20°C, dark and running water, n=3).

Supplementary Fig. S3

Figure S3Price equation partitioning of community change for total FL associated with Chl-a (mV). For details of the plot, see the main text.