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Mammalian Cell Bank Characterization
(Form YY)
Please print and complete this form and forward/fax to MDS. We will contact you for a detailed quotation.
Institution: / Investigator:Email:
Billing Address:
Submitted by: / Date Submitted // / PO #:
Shipping Address:
Zip: / State: / City:
Fax: / Tel: / Ext:
Type of Cells: HumanAnimal
PrimaryImmortalTransformed/Transgenic
Master Cell Bank (MCB)Working Cell Bank (WCB)End of Product Cell Bank EPCB)
ID as it appears on the submitted sample:
Culture medium to be used:
Culture Reagent / Type / Manufacturer / Cat. # / Final Concentration in Culture MediumMedium
Serum
Additional Supplements
Environmental conditions:
Temperature: Humidity: %CO2:
AdherentNon-adherent
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Identity Testing
Karyotyping MCB WCBEPCB
Genetic profiling (STR) MCB WCBEPCB
Total viable cells/vial MCBWCBEPCB
Cell viability (post-bank thaw) MCB WCBEPCB
Isoenzyme electrophoresis MCBWCBEPCB
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Glucose-6-phosphate dehydrogenase (G6PD)
Lactate dehydrogenase (LD)
Malate dehydrogenase (MD)
Nucleoside phosphorylase (NP)
Aspartate aminotransferase (AST)
Peptidase B (PepB)
G-Protein coupled receptors (GPCR)
cAMPphosphodiesterase (cAMP)
Protein kinase (PK)
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Purity
In vitro adventitious virus testing MCBWCBEPCB
In vivo adventitious virus testing MCBWCBEPCB
In vitro Assay, Detection of porcine Viral Contaminants using PPK cells According to 9CFR
MCBWCBEPCB
F-PERT Assay MCBWCBEPCB
Retrovirus 293 Co-cult (5 Passages, FPERT End-point) MCBWCBEPCB
PCR testing for human viruses and Mycoplasma for MCB WCBEPCB
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EBV (HHV 4, Human Herpes Virus, Epstein-Barr)
Hepatitis A Virus (HAV)
Hepatitis B Virus (HBC)
Hepatitis C Virus (HCV)
Human Cytomegalovirus (CMV)
Human Herpes Virus 1 (HHV1)
Human Herpes Virus 6 (HHV6)
Human Herpes Virus 7 (HHV7)
Human Herpes Virus 8 (HHV8)
Human Immunodeficiency Virus-1 (HIV1) DNA
Human Immunodeficiency Virus-1 (HIV1) RNA
Human Immunodeficiency Virus-2 (HIV2) DNA
Human Immunodeficiency Virus-2 (HIV2) RNA
Human Papilloma virus -16 (HPV-16)
Human Papilloma virus -18 (HPV-18)
Human Rotavirus (HRV)
Human T-Cell Leukemia Virus Type 1 (HTLV1) DNA
Human T-Cell Leukemia Virus Type 1 (HTLV1) RNA
Human T-Cell Leukemia Virus Type 2 (HTLV2) DNA
Human T-Cell Leukemia Virus Type 2 (HTLV2) RNA
Influenza
Measles
Norwalk Virus (NW)
Parvovirus B19 (Parvo-B19)
Rubella
Enterovirus (polio)
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PCR testing for bovine virusesMCBWCBEPCB
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Bovine viral diarrhea virus (BVDV)
Bovine parvovirus (BPV)
Bovine adenovirus (BAV)
Bluetongue virus (BTV)
Bovine respiratory syncytial virus (BRSV)
Reovirus (RV)
Rabies virus
Bovine Herpes Virus I (BHV I)
Bovine Herpes Virus IV (BHV IV)
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PCR testing for porcine virusesMCBWCBEPCB
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Simian viruses (SV40 and SV-5)
African swine fever virus (ASFV)
Classical swine fever virus (CSFV)
Foot and mouth disease virus (FMDV)
Japanese encephalitis virus
Swine vesicular disease (SVD)
Vesicular exanthema (VE)
Vesicular stomatitis
Porcine Circovirus-1 and 2 (B/PCV1)
Swine hepatitis E Virus (HEV)
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Testing for animal viruses according to 9 CFR 113.46, 113.47 and 113.53, using cell culture monolayers MCB WCB EPCB
Testing serum alone for bovine diarrhea virus (BVDV1) and bovine rotavirus (BRV)
Testing trypsin alone for Porcine Parvovirus (PPV)
Testing product for both porcine and bovine viruses
Basic MicrobiologyMCBWCBEPCB
Bacteriostasis/Fungistasis (B & F)
Sterility (Direct Inoculation Method (EP, USP, JP and 21 CFR)
Endotoxin (LAL)
Mycoplasma PCR
Direct Culture and DNA Staining Test for the Detection of Mycoplasmas
TEM Examination of Cell Cultures (200 Cell profiles)MCBWCBEPCB
In vivo tumorigenicity MCB WCBEPCB
In vivo Oncogenicity MCBWCBEPCB
Additional Testing: