CLINICAL LABORATORY TECHNICIAN PROGRAM

MadisonvilleCommunity College

Task List

STUDENT: / DATE:
PROCEDURE: / MICROBIOLOGY CLINICALS / GRADE:
TASK TO PERFORM / Policy, Rules, Procedures Explained by Instructor / Performed Under Supervision / Performed Independently / Needs Improvement / Not performed at this clinical facility
Select appropriate media for each type of specimen received for culture, using protocol manual.
Urine plating
Sputum/Respiratory
  • screening protocol/rejection

Stool
  • preservation and stool media

Body Fluid Cultures
  • CSF and other fluids
  • Centrifugation/media

Miscellaneous Cultures
  • Cath Tips
  • Wound cultures
  • Backup Strep cultures

MRSA Screens
O&P Crypto/Giardia Screens
  • Preservative/SAF

Group B (LIM) cultures
Blood Cultures
  • Bottle data entry
  • Positive Bottle
  • Gram Stain, reporting in LIS
  • Doctor notification/alert value

Microscopy:
  • Gram Stains
  • KOH/Wet Preps
  • India Ink (CSF)
  • Arthropod Exams
  • AFB Staining (KF & Flurochrome)

Perform incubation of cultures under proper conditions for optimal growth and interpret culture and stain characteristics to detect normal flora, potential pathogens/contaminants.
Urine Cultures
  • Contamination protocol
  • Colony counts

Respiratory Cultures
  • Pathogens vs. usual respiratory flora
  • Yeast Protocol

Stool Cultures
  • Pathogens vs. usual fecal flora
  • State lab Isolate Prep

Miscellaneous Cultures such as:
  • Usual Flora vs. True Pathogens
  • Blood Bank Cultures
  • Dialysis Water cultures
  • Foreign Objects
  • Cath-tip Cultures (colony count)

Wound Cultures
  • True pathogens vs. usual flora

Anaerobic Cultures
  • Aerotolerance Testing
  • ID Testing
  • Cefinase Testing

Blood Cultures
  • Contamination vs. pathogens
  • Instrument Maintenance

Perform rapid screening tests/identification tests when appropriate.
Catalase test
Slide coagulase test or staph latex test
Staph latex test
Hippurate test
Candida screen
Optochin Test
PBP2 for MSRA
Oxidase test
Rapid strep screen
Rapid indole test
Flu A & B tests
Legionella Urine Antigen
Pneumococcal Urine Antigen
Mycoplasma Antibody
Crypto/Giardia Antigen
Vaginosis DNA Probe Testing (if applicable)
Occult Blood
Bacterial Antigen Panel
Gastroccult
Fecal Lactoferrin (WBC)
Perform susceptibility test procedures.
Walkaway Panels
  • Inoculum Prep
  • Drug selection/reporting
  • Purity testing

E-Test MIC’s
  • Inoculum prep
  • Drug selection

MDRO Protocols
Instrument maintenance
Prepare organisms for identification using an automated system.
Walkaway Panel Selection
Instrument Maintenance
Observe Mycobacteriology workup (if applicable).
  • Specimen Digestion/concentration procedures
  • LJ & MGIT Examination
  • Procedures for Positives

Observe Mycology workup (if applicable).
  • Yeast Protocol
  • Lactophenol Cotton Blue Prep Procedure
  • Contamination vs. pathogen
  • Subculturing for dermatophytes
  • India Ink Prep

Ova & Parasite Examination (if applicable).
  • Trichrome Stain
  • Concentration Procedure
  • Iodine Prep
  • Review old Proficiency testing samples/slides

Perform identification testing to include:
Comments:

09/06