© 2014
M. Mirzaeva –Ph. D student
(Scientific adviser− Doctor of Veterinary Science S. Kulinych)
PoltavaStateAgrarianAcademy
EFFECTIVENESS OF LOCAL APPLICATION OF COLLOID OF NANOCLUSTERS AQUACHELATE OF METALS (Ag, Cu, Zn, Mg) IN TREATMENT OF PARODONTOPATHY CONDITIONS IN DOGS
Readers − Doctor of Veterinary Science A. Zamaziy
In workwasclear upin dynamics the results of individualmorphological indexesof krevicular fluid in comparison under using colloid of nanoclustersakvahelat ofmetals (Ag, Cu, Zn, Mg) and chlorhexidine digluconate (0,05% solutionby the treatment of the early forms of parodontopathy in clinically sick dogs. By ascertainmentthe comparative effectiveness we were compared: quantitativelyand qualitatively dynamics of desquamative pavement epithelium and forms of leukocytes.While topical use of a colloid of nanoclusters recorded gradual normalization of these indexes.
Key words: a dog, krevicular fluid, desquamative pavement epithelium and forms of leukocytes.
Statement of a problem.Periodontal disease is dominated by its intensity, spreading and destroying effects on the animals dentition in veterinary dentistry. As a result, on present stage of development of veterinary dentistry appearsa problem insearchingof effective methodsintreatmentof inflammatory periodontal diseases in dogs [1, 2, 3].
In particular, one of the following methods of local treatment is the use of nanoclusters akvahelate ofmetals [4, 5, 6, 7, 8].
Analysis of major studies and publications which discuss the objects.Up topresenttime One of the main preparations for local treatment of parodontopathyretained chlorhexidine digluconate [9]. Butfrequentlybyits usebecome apparentsuchcomplications as teeth enamel staining, increased deposition of dental tartar, taste disorder [10].
Uponthis dayin veterinary medicine has accumulated a fairly large statistical material concerning the treatment of periodontal disease in dogs[1-3], but attention was sparenot enough to the introduction of newestpreparationsfortreat givenpathology, in particularnanoclusters akvahelate of metals, which are frequentlyused practise medicine[4, 7, 8, 11].
Among the advantages of typicalyfor nanomaterials should distinguish their biological compatibility and ecological cleanliness thanks which it is possibleconsiderablyexpanded the range of application of nanotechnology products in various branches of veterinary medicine [12].
Search methods for prevention and treatment of animal diseases, based on the use nanoparticles of metals and represents an important scientific and practical interest [13].
Research objectives.For establishmentthe efficacy of preparation under the treatment of the early forms of parodontopathy in clinically affected dogsformed two groups of animals. In the control group (n = 5) chlorhexidine digluconate (0,05% solution) applied, and nanoclustersakvahelat metals (Ag, Cu, Zn, Mg) local used in experimental group (n = 3) [8]. To qualifycomparative efficacy thefollowing local treatment under the means of quantitativeand qualitativecalculation of individual morphological parameters: dynamics of desquamative pavement epithelium and forms of leukocytes in krevicular fluid.
Materials and methods.Staging experiment conducted with dogs of different breeds 4–12 years of age, for ten-days monitoring parameters Triasstic. On dogs of both groups krevicular fluids were sampled in the morning on an empty stomach.
Samples of substance for morphological investigations was obtained according to methods of S. Eryna, S. Dyachkova and also E. Zhulev, A. Serov [14,15]. Adapted by taking samples from the gingival pocket approximative surfaces of canines and premolars with signs of parodontopathy using sterile endodontic paper pins ISO 30 (Meta Biomed, United Kindom), previously the surrounding tissue were drained with sterile gauze tampons, ten pins were administered to the bottom of the pocket and left there for 5 minutes. Next pins was transferred to a test tube containing 200 ml of physiological sodium chloride solution, was left for 30 minutes to flush its components, then pins were removed and within two hours research was conducted (to avoid destruction of the material).
For quantitative calculation – krevicular fluid diluted in a solution of NaCl, was placed in solution of Turk at a ratio of 10:1, then was stirred and brought to the camera of Goryaev. Cellular elements were counted throughout the area of the grid of chamber [16]. For qualitativecalculation – diluted krevicular fluid in physiological sodium chloride solution, was fixed on a slide stained according to Romanovsky-Himza. The number of desquamative pavement epithelium was counted and also forms of leukocytes were differentiated throughout the area of glass [17, 18].
After sedation, in animals were removed dental deposition using dental ultrasonic scalers (WOODPECKER, DPRK), and then spent polishing teeth with cosmetic dental instrumentation Dental Polisher, (Profi White, DPRK). After the procedure, the oral cavity aerosol treated: in control group, 0,05% solution of chlorhexidine digluconate(CP "Luhanskregional "Pharmacy", Ukraine) – and in experimental group colloid of nanoclustersakvahelat of metals(Ag, Cu, Zn, Mg). Research conducted samplesof krevicular fluid: the first time before the ultrasonic cleaning of teeth and beforethe first spray solutions in both of groups, the second time on the 6th, and the third time on the 17th day. Oral cavity treated once a day daily.
Studies.The researchresults shows that byquantitative calculationof morphological krevicular fluid clinically affected dogsin the experimental group on the first day quantitative of desquamative pavement epithelium was 8,00 ± 1,07 pcs.,that was lower on 20,0% thanin control group (10,00±1,26pcs.). On the sixth day index in the control group reduced comparativeto output data to 5,60 ± 0,86 pcs., and was lower on 30,0% than in the experimental (8,66 ± 1,26 pcs.). On the 17th day in the control groupquantitativeof desquamative pavement epithelium was higher on 33,4% than in the experimental group, which was 7,33 ± 0,84 pcs., regarding to output data.
The number of leukocytes in the control group was 17,60 ± 1,72 pcs., whathigher on 21,6% than in research group. On the sixth day quantitativeof leukocytes in the control group increased concerning to output data 22,60 ± 4,08 G / l – testify of acute inflammation in the oral cavity and was on 54,6% higher than in the experimental group 10,00 ± 0,84 G / l (p0,05). Until the last day of treatment in the experimental group index was at 6,66 ± 0,42 G / l and was lower on 39,5% than in the control group (tabl.).
Table
Dynamics of morphological krevicular fluid in clinically sick dogs
in early forms of parodontopathy (M ± m)
1st day / 6th day / 17th day
c
n = 5 / e
n = 3 / c
n = 5 / e
n = 3 / c
n = 5 / e
n = 3
Desquamative pavement epithelium, units.in the chember / 8,00±1,07 / 10,00±1,26 / 5,60±0,86 / 8,66±1,26 / 11,40±3,00 / 7,33±0,84
Leukocytes,G / lin the chember / 17,60±1,72 / 13,33±1,26 / 22,60±4,08 / 10,00±0,84* / 11,60±2,36 / 6,66±0,42
Desquamative pavement epithelium,units.in the stained smears / 14,80±3,86 / 18,33±1,26 / 9,60±2,80 / 10,33±0,42 / 13,80±1,29 / 11,33±0,42
Lymphocytes,units. in the stained smears / 4,40±0,21 / 4,33±0,42 / 8,80±1,50 / 4,00±0,84* / 4,00±0,43 / 3,66±0,42
Neutrophils,
units. in the
stainedsmears / 15,00±1,29 / 16,33±1,26 / 5,40±1,72 / 11,66±0,42* / 6,40±0,64 / 11,00±0,42***
Note: * – р0,05,*** – р0,001, whereрconcerning to control group.
The results of research the quantityof desquamative pavement epithelium and leukocytes on the 17th day indicate a tendency to reduce the number of index in the experimental group regarding to the control that indicatesabout more efficiencyof use of nanoclusters as leukocyte activity and increased quantity of desquamative pavement epithelium in krevicular fluid are markers of inflammation.
By qualitativecalculationcellular elements the index desquamative pavement epithelium on the first day in the experimental group was 17,8% higher than in the control group. By the sixth day marked index increased on 4% in the experimental group (10,33±0,42 pcs.) regarding to the controlgroup
(9,60±2,80 pcs.). On the 17th day in the control group, they number was 13,80 ± 1,29 pcs., that on 12,9% more than in research group. It should be noted that lymphocytes in the control group and in the experimental group was on the same level. On the sixth day in the control groupindex was higher on 50%
(8,80 ± 1,50 pcs.) than in research group (4,00 ± 0,84 pcs., p0,05), regarding to the output data. On the 17th day in the control group were more on 8,5% of lymphocytes than in experimental group.
It should be noted that in the process of therapy quantity of neutrophils throughout the area of glass in the experimental group on the first day was16,33 ± 1,26 pcs., which on 6,3% more than in the control group. On the sixth day in the control groupconcerning to the output data recorded 5,40 ± 1,72 pcs., which on 51,0% less than in the research group (11,66 ± 0,42 pcs., p0,05). On the 17th day in the experimental group quantityincreased to 11,00 ± 0,42 units. (p0,001), which is higher than in the control group on 41,8%.
Conclusions.Thus, we analyzed the results and may conclude, that in the process of localapplicationof colloid nanoclustersakvahelat of metalsby the treatment of the early forms of parodontopathy in clinically affected dogs observed gradual normalization quantitativelyand qualitatively:desquamative pavement epithelium and forms of leukocytes, which enterto cellular composition krevicular fluid on, unlike index by use of chlorhexidine digluconate(0,05% solution),that testify aboutattenuationof the inflammatoryreaction.
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