LOCUS Exported File 2264 Bp Ds-DNA Circular SYN 05-MAR-2013
LOCUS Exported File 2264 bp ds-DNA circular SYN 05-MAR-2013
DEFINITION Entry vector for transferring a cloned attL-flanked gene to a
destination vector in the Gateway(R) system.
ACCESSION .
VERSION .
KEYWORDS pENTR201
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2264)
AUTHORS I.M.A.G.E. Consortium
TITLE Direct Submission
JOURNAL Exported Saturday, Apr 06, 2013 from SnapGene 1.5.1
FEATURES Location/Qualifiers
source 1..2264
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
terminator 80..107
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
/note="color: #ffffff"
terminator 199..285
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
/note="color: #ffffff"
protein_bind 338..436
/bound_moiety="LR Clonase(TM)"
/note="attL1'"
/note="recombination site for the Gateway(R) LR reaction;
mutated version of attL1"
/note="color: #31849b"
protein_bind 447..546
/gene="mutant version of attL"
/bound_moiety="LR Clonase(TM)"
/note="attL2"
/note="recombination site for the Gateway(R) LR reaction"
/note="color: #31849b"
CDS 669..1478
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin in bacteria or G418
(Geneticin(R)) in eukaryotes"
/note="color: #ccffcc"
/translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
rep_origin 1621..2209
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
ORIGIN
1 acatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgctagccagg
61 aagagtttgtagaaacgcaaaaaggccatccgtcaggatggccttctgcttagtttgatg
121 cctggcagtttatggcgggcgtcctgcccgccaccctccgggccgttgcttcacaacgtt
181 caaatccgctcccggcggatttgtcctactcaggagagcgttcaccgacaaacaacagat
241 aaaacgaaaggcccagtcttccgactgagcctttcgttttatttgatgcctggcagttcc
301 ctactctcgcgttaacgctagcatggatctcgggccccaaataatgattttattttgact
361 gatagtgacctgttcgttgcaacaaattgatgagcaatgcttttttataatgccaacttt
421 gtacaaaaaagctggcaccatgggcgacccagctttcttgtacaaagttggcattataag
481 aaagcattgcttatcaatttgttgcaacgaacaggtcactatcagtcaaaataaaatcat
541 tatttgccatccagctgcagctctggcccgtgtctcaaaatctctgatgttacattgcac
601 aagataaaaatatatcatcatgaacaataaaactgtctgcttacataaacagtaatacaa
661 ggggtgttatgagccatattcaacgggaaacgtcgaggccgcgattaaattccaacatgg
721 atgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaa
781 tctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggta
841 gcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgc
901 ctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactg
961 cgatccccggaaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaata
1021 ttgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtc
1081 cttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtt
1141 tggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctgga
1201 aagaaatgcataaacttttgccattctcaccggattcagtcgtcactcatggtgatttct
1261 cacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgag
1321 tcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagtttt
1381 ctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaata
1441 aattgcagtttcatttgatgctcgatgagtttttctaatcagaattggttaattggttgt
1501 aacactggcagagcattacgctgacttgacgggacggcgcaagctcatgaccaaaatccc
1561 ttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttc
1621 ttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctacc
1681 agcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctt
1741 cagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccactt
1801 caagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgc
1861 tgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataa
1921 ggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgac
1981 ctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagg
2041 gagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggga
2101 gcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgact
2161 tgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaa
2221 cgcggcctttttacggttcctggccttttgctggccttttgctc
//