LOCUS Exported 8788 bp ds-DNA circular SYN 02-JAN-2015
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pZIP-hEF1a-HpaI-empty
SOURCE synthetic DNA construct
ORGANISM recombinant plasmid
REFERENCE 1 (bases 1 to 8788)
AUTHORS Transomic
TITLE Direct Submission
JOURNAL Exported Wednesday, Feb 04, 2015 from SnapGene 2.6.2
FEATURES Location/Qualifiers
source 1..8788
/organism="recombinant plasmid"
/mol_type="other DNA"
misc_feature 23..667
/note="CMV-LTR"
misc_feature 720..856
/note="psi"
misc_feature 1348..1552
/note="rre"
misc_feature 2062..2212
/note="cts"
misc_feature 2213..2214
/note="MCS"
misc_feature 2220..3406
/note="pEF1 alpha"
primer_bind 3415..4110
/note="seq.SFFVfw"
CDS 3415..4110
/codon_start=1
/label=ZsGreen
/note="ZsGreen"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
primer_bind 3415..3480
/note="seq.ZsGrev"
primer_bind 4005..4110
/note="seq.ZsGfw"
misc_feature 4122..4698
/note="IRES"
CDS 4699..5301
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAKLSGSRL
AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 5319..5399
/note="5'-UltramiR"
misc_feature 5400..5473
/label=Ultramir3'
/note="Ultramir3'"
misc_feature 5493..6084
/note="WPRE"
misc_feature 6289..6524
/note="3'LTR"
promoter 6618..6947
/note="SV40"
/note="SV40 enhancer and early promoter"
rep_origin 6798..6933
/note="ori"
/note="SV40 origin of replication"
rep_origin complement(7036..7621)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7792..8751)
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
ORIGIN
1 gacggatcgggagatcctcgcgcgttgacattgattattgactagttattaatagtaatc
61 aattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggt
121 aaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgta
181 tgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacg
241 gtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattga
301 cgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactt
361 tcctacttggcagtacatctacgtattagtcatcgctattaccagctagcgaggcgtggc
421 ctgggcgggactggggagtggcgagccctcagatcctgcatataagcagctgctttttgc
481 ctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagg
541 gaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccg
601 tctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaat
661 ctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctct
721 cgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtg
781 agtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtc
841 agtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccaggggga
901 aagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgca
961 gttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaa
1021 ccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctc
1081 tattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagag
1141 gaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagac
1201 ctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaa
1261 aaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaa
1321 aaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcacta
1381 tgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgc
1441 agcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacag
1501 tctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatc
1561 aacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgcctt
1621 ggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatgg
1681 agtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgc
1741 aaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgt
1801 ggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtag
1861 gaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggc
1921 agggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggc
1981 ccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtga
2041 acggatctcgacggtatcgcctttaaaagaaaaggggggattggggggtacagtgcaggg
2101 gaaagaatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaatt
2161 acaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttatcgatac
2221 gtgaggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagtt
2281 ggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactggga
2341 aagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataag
2401 tgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaag
2461 tgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttg
2521 aattacttccacgcccctggctgcagtacgtgattcttgatcccgagcttcgggttggaa
2581 gtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttg
2641 aggcctggcttgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtc
2701 tcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgcttt
2761 ttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggttt
2821 ttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggg
2881 gcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctc
2941 tggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggt
3001 cggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaa
3061 aatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaaggg
3121 cctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggc
3181 acctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggtttt
3241 atgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcact
3301 tgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagc
3361 ctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgcccggtccatggcc
3421 cagtccaagcacggcctgaccaaggagatgaccatgaagtaccgcatggagggctgcgtg
3481 gacggccacaagttcgtgatcaccggcgagggcatcggctaccccttcaagggcaagcag
3541 gccatcaacctgtgcgtggtggagggcggccccttgcccttcgccgaggacatcttgtcc
3601 gccgccttcatgtacggcaaccgcgtgttcaccgagtacccccaggacatcgtcgactac
3661 ttcaagaactcctgccccgccggctacacctgggaccgctccttcctgttcgaggacggc
3721 gccgtgtgcatctgcaacgccgacatcaccgtgagcgtggaggagaactgcatgtaccac
3781 gagtccaagttctacggcgtgaacttccccgccgacggccccgtgatgaagaagatgacc
3841 gacaactgggagccctcctgcgagaagatcatccccgtgcccaagcagggcatcttgaag
3901 ggcgacgtgagcatgtacctgctgctgaaggacggtggccgcttgcgctgccagttcgac
3961 accgtgtacaaggccaagtccgtgccccgcaagatgcccgactggcacttcatccagcac
4021 aagctgacccgcgaggaccgcagcgacgccaagaaccagaagtggcacctgaccgagcac
4081 gccatcgcctccggctccgccttgccctgagaattcaattccgcccctctccctcccccc
4141 cccctaacgttactggccgaagccgcttggaataaggccggtgtgcgtttgtctatatgt
4201 tattttccaccatattgccgtcttttggcaatgtgagggcccggaaacctggccctgtct
4261 tcttgacgagcattcctaggggtctttcccctctcgccaaaggaatgcaaggtctgttga
4321 atgtcgtgaaggaagcagttcctctggaagcttcttgaagacaaacaacgtctgtagcga
4381 ccctttgcaggcagcggaaccccccacctggcgacaggtgcctctgcggccaaaagccac
4441 gtgtataagatacacctgcaaaggcggcacaaccccagtgccacgttgtgagttggatag
4501 ttgtggaaagagtcaaatggctctcctcaagcgtattcaacaaggggctgaaggatgccc
4561 agaaggtaccccattgtatgggatctgatctggggcctcggtgcacatgctttacatgtg
4621 tttagtcgaggttaaaaaaacgtctaggccccccgaaccacggggacgtggttttccttt
4681 gaaaaacacgataataccatggccaccgagtacaagcccacggtgcgcctcgccacccgc
4741 gacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacg
4801 cgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttc
4861 ctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtg
4921 gcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggctcg
4981 cgcatggccaagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctg
5041 gcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgac
5101 caccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgc
5161 gctggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcgg
5221 ctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatg
5281 acccgcaagcccggtgcctgagcggccgcaatggatcctgtttgaatgaggcttcagtac
5341 tttacagaatcgttgcctgcacatcttggaaacacttgctgggattacttcttcaggtta
5401 actgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaa
5461 ttaaatcactttacgcgtggcgcgcctggaacaatcaacctctggattacaaaatttgtg
5521 aaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctt
5581 taatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtata
5641 aatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtgg
5701 tgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagc
5761 tcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcct
5821 gccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgt
5881 cggggaagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcg
5941 ggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcc
6001 tgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatct
6061 ccctttgggccgcctccccgcctggaattaattctgcagtcgagacctagaaaaacatgg
6121 agcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcaca
6181 agaggaggaggaggtgggtttttccagtcacacctcaggtacctttaagaccaatgactt
6241 acaaggcagctgtagatcttagccactttttaaaagaaaagaggggactggaagggctaa
6301 ttcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagacc
6361 agatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaa
6421 gcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactaga
6481 gatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatc
6541 ttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggccttgac
6601 attgtttaaactggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcag
6661 gcagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccag
6721 gctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtcc
6781 cgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccc
6841 atggctgactaattttttttatttatgcagaggccgaggccgcctctgcctctgagctat
6901 tccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagctcccgggag
6961 cttgtatatccattacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc
7021 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg
7081 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg
7141 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt
7201 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt
7261 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg
7321 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact
7381 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt
7441 cttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctct
7501 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac
7561 cgctggtagcggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctca
7621 agaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtta
7681 agggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaa
7741 atgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatg
7801 cttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctg
7861 actccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgc
7921 aatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagc
7981 cggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaa
8041 ttgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
8101 cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccgg
8161 ttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctc
8221 cttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttat
8281 ggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactgg
8341 tgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgccc
8401 ggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattgg
8461 aaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgat
8521 gtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgg
8581 gtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatg
8641 ttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtct
8701 catgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcac
8761 atttccccgaaaagtgccacctgacgtc
//