Legends for supplemental figures
Supplemental Figure 1. Design of the in vivo study evaluating the 30 days effects of OBST injection in healthy and type 2 diabetic mice. Mice were fed 10 weeks standard diet (healthy) and 60% High Fat Diet - HFD diet (T2DM). After this time, T2DM animals were injected of a single dose of streptozotocin (50 mg/kg body weight).After 7 days, animals with blood glucose level above 11 mmol/l were considered diabetic. Next,healthy and T2DM mice were divided into two groups: vehicle (administrated with 0.9% NaCl) and OBST (administrated with 75 nmol/kg b.w. of OBST). Animals were receiving saline or obestatin for 30 days. During the experiment healthy animals were fed a standard diet and T2DM animals received HFD. Next, animals were sacrificed and specimens (blood and tissues) were collected.
Supplemental Figure 2. Randomized body weight and blood glucose before experiment. Body weight at the start of the experiment (A). The mean of body weight was 24.18 ±0.34 g for vehicle (0.9% NaCl) and 24.31±0.36 g for obestatin treated healthy group and 27.79±1.39 g for HFD/STZ control and 27.89±1.008g for obestatin treated HFD/STZ group. Body mass was statistically significant higher in T2DM animals compared to healthy mice. There were no differences in vehicle and OBST group in healthy and T2DM mice also. Blood glucose before experiment (B). Glucose level ranged 5.64±0.715mmol/l (0.9% NaCl), 5.69±0.723mmol/l (OBST) in healthy mice and 13.24±1.024 mmol/l (0.9% NaCl) and 13.27± 0.645 mmol/l (OBST) in T2DM animals.Statistically significant changes are marked *p<0.05, **p<0.01 and *** p<0.001.
Supplemental Figure 3.Mean of food consumption and serum obestatin level after experiment.Average of food intake in healthy and T2DM animals (A). Food intake was monitored for each cage, not individually for eachmice.Serum obestatin level after experiment (B). Statistically significant changes are marked *p<0.05, **p<0.01 and *** p<0.001.