Intermediate/advanced workshop (IMED 7302) – (Revised 11Feb9)

June 6-14, 2011

DATE / TIME / LOCATION / DESCRIPTION
June 6 / 8.30
9:00-12.00
12.00-1.00
1.00-3.30
3.30-5.30 / Thompson Rm*
ON6045
Thompson Rm*
ON 6045
GCCRD (ON6026) / Dr. Sabine Mai: Introduction
In the Lab:
Chromosome preparation and cell preparation for fluorescent immunohistochemistry
LUNCH
Fluorescent immunohistochemisrty
Start of Imaging
June 7 / 8.30-9.30
9.30-11.00
11.00-12.00
12.00-1.00
1.00-2.00
2.00-2.45
2.50-3.35
3.45-5.30
5.30-6.30 / Thompson Rm*
ON 6045
Thompson Rm*
Thompson Rm*
ON 6045
Theatre A
Theatre A
Theatre A
Thompson Rm* / Dr. Sabine Mai: 2D/3D-FISH - Overview
In the Lab:
3D Q-FISH (step 1)
Dr. Maya Everett: Overview of 3D techniques
[Q-FISH hybridization, 2h, step 2]
LUNCH
3D Q-FISH (step 3 - end)
Dr. Peter Jansson – Lecture: Introduction to Optics: Waves, Rays and Optical Imaging Systems
Dr. Peter Jansson – Lecture: Introduction to Deconvolution
Dr. Maya Everett: Leading Edge in Imaging
Dr. Maya Everett: Journal Club
June 8 / 8.30-12.00
12.00-12.30
12.45-1.45
2.00-5.00 / GCCRD
Thompson Rm*
Thompson Rm*
ON 6045 / 3D/2D imaging and analysis
LUNCH
Dr. Michael Koehler – Lecture: Title?
In the lab: SKY and mBAND – day 1
June 9 / 8.30-10.30
10.30-12.00
12.00-1.00
1.00-3.00
3.00-3.15
3.30-4.30 / Thompson Rm*
Lecture Theatre ON2134
GCCRD
GCCRD
Thompson Rm / Journal clubs:
1)Dr. Michael Koehler: selected paper?
2)Dr. Sabine Mai: Wiener et al., Genes & Cancer, 2010
Dr. Yuval Garini – Lecture: Organization mechanisms of the genome in the nucleus studied through telomeres and DNA diffusion
LUNCH
2D/3D imaging/analysis
group photo
Journal Club – Dr. Yuval Garini: Misteli T. Science 291: 843-847.2001.
June 10 / 9:00-1:00
9.00-9.10
9.10-9.50
9.50-10.30
10.30-11.10
11.15-11.40
11.40-12.20
12.20-1.00
1.00-2.00
2.00-5.30 / Lecture Theatre ON2134
Outside Lecture Theatre ON2134
Lecture Theatre ON2134
GILMI RM**
ON6045 and
GCCRD / MINI SYMPOSIUM “Genomic Instability and Cancer”:
Dr. Sabine Mai: Introduction
Dr. Gissellsson Nord (Lund University, Sweden
– “Pathways to aneuploidy in cancer”
Dr. Thomas Ried (NIH/NCI, Bethesda, USA): – “ Aneuploidy dependent transcriptional deregulation of the cancer transcriptome”
Dr. Kirk McManus (MICB, U of M): – “Defects in Sister Chromatid Cohesion Genes may Underlie Colorectal Cancer”
COFFEE
Dr. Sabine Mai (MICB/ CCMB/U of M, Winnipeg, Canada): – “Nuclear remodeling and genomic instability in cancer”
Dr. Hans Knecht (CHUS/U de S, Quebec, Canada): “3D Telomere Dynamics in Hodgkin's Lymphoma: Closer to the Secret of the Reed-Sternberg cell”
LUNCH
In the lab: SKY wash and mBAND wash
Start imaging
June 13 / 9.00-12.00
12.00-1.00
1.00-5.00 / GCCRD
Thompson Rm*
GCCRD / Imaging/analysis
LUNCH
Imaging/analysis/data summaries
June 14 / 9.00-12.00
12.00-1.00
1.00-3.00 / Thompson Rm*
Thompson Rm*
Thompson Rm* / Data summaries/discussion
LUNCH
TEST

*THOMPSON ROOM – ON6005, CancerCare Manitoba

**GIMLI ROOM – ON1188, CancerCare Manitoba

***BOARD ROOM – ON4017, CancerCare Manitoba