In Subfamilies (Based on Figure 1). Positively Charged Regions 1 and 2 Are Indicated By

Supplementary Figure S1. Alignments used in phylogenetic analyses. * indicates conserved derived amino acid residues

in subfamilies (based on Figure 1). Positively charged regions 1 and 2 are indicated by bold italics.

A. N-lobes

O subfamily 60

NP_990635_Gallus_gallus APPKSV-IRW CTISSPEEKK CNNLRDLTQQ ERISLT------CVQKAT YLDCIKAIAN

XP_003209092_Meleagris_gallopavo APPKTT-IRW CTISPLEEKK CNNLKDLTQQ ERITLT------CLQKAT YLDCIKAIAN

XP_005143141_Melopsittacus_undulatus APPKAS-IRW CTISSAEENK CNSLRDHMQQ ENTALS------CLQKAT YLDCIKAISN

XP_005489599_Zonotrichia_albicollis APQKPS-VRW CTISSVEEKK CNSLKDQMQQ ENFAFS------CLQKAS YLDCIKAISN

XP_002192726_Taeniopygia_guttata APQKPS-VKW CTVSSAEEKK CNSLKDHMQQ ENFAFS------CLQKAS YLDCIKAISN

BAJ23165_Dromaius_novaehollandiae AAPKAT-VRW CTISSPEQKK CNDLKERMQQ ESFGLS------CVQKTT YMDCIMAIAR

CAH10347_Struthio_camelus APPKPI-IRW CTISPAEQKK CSELKQLMQQ ESVGLT------CVQKTT YLDCITAISR

XP_005050798_Ficedula_albicollis APQKPS-VRW CTISSAEEKK CNSLRDHMQQ ENFAFS------CLEKAS YLDCIKAISN

XP_005303689_Chrysemys_picta_bellii DPPAQSPVRW CTISDPEQKK CVELKTKMSS -TPSLD------CVKKTT YSDCIKAIAE

XP_006037143_Alligator_sinensis APQAQKDIKW CTISNPEQEK CLKMRKAMAS DTTSPP---- C--NCVQKGS HSECIKAIAA

CAK18228_Lacerta_agilis T----SGVRW CTTSTAEQNK CQRL-QECLS ASQQPASFPQ F--SCVKKSS PHDCISAIAA

CAK18230_Crocodylus_niloticus APQAQEDIRW CTRSDKEQMK CLELKKAMFP DHRPPPP--P F--DCVQRDS HTACIEAISS

CAK18222_Chamaeleo_chamaeleon T----SPVRW CTTSAPEHAK CQRL-QDCFS -SQRSENLPS F--SCVRKTD SHDCIRAISN

XP_003218456_Anolis_carolinensis T----SPVRW CVTSDPEQAK CRRL-HDCFS -GQQSENLSQ L--SCVKRSD AHDCIKAIAN

CAK18224_Python_bivittatus T----SSVRW CTISNEEQAK CTRLQQECFP -PQQSKELPV L--NCVRKTD HQDCIRAIKN

CAK18223_Boaedon_fuliginosus S----SPVRW CTISTEEQEK CQRLKQECFS -QQQSKDFPE L--ICVRKTD HQECITAIKN

CAK18221_Natrix_natrix T----SSVRW CTVSNEEQEK CQRLKQECFA -QQQSKDFPE P--ICVRKTD HQECITAIKN

S subfamily

XP_005600539_Equus_caballus ----EQTVRW CTVSNHEVSK CASFRDSMKS IVP-AP--PL V--ACVKRTS YLECIKAIAD

XP_534268_Canis_lupus_familiaris VS-PEKTVRW CTVSNHEASK CSSFMENMKT VLE-NG--PF V--SCVKRTS YLECIKAIWA

XP_003265287_Nomascus_leucogenys V--PDKTVRW CAVSEHEATK CQSFRDHMKS ILPSNG--PS V--ACVKKTS YLDCIRAIAA

XP_005675608_Capra_hircus D--PEKTVRW CTISTHEANK CASFRENMLR IFE-NG--PF V--SCVKKTS HMDCIKAISN

NP_001054_Homo_sapiens V--PDKTVRW CAVSEHEATK CQSFRDHMKS VIPSDG--PS V--ACVKKAS YLDCIRAIAA

NP_803450_Bos_taurus D--PERTVRW CTISTHEANK CASFRENVLR ILE-SG--PF V--SCVKKTS HMDCIKAISN

XP_003476776_Cavia_porcellus V--PEKTVRW CAVLDHEASK CASFRDVMKK VLPPEG--PR V--VCVKKNS HLDCIKSIAA

XP_004419415_Ceratotherium_simum_simum ----DQTVRW CTVSNHEGSK CSSFRDSMKS VLP-AG--PS V--VCVRRNS YIECIKAIAD

XP_004603284_Sorex_araneus S--PEQTVRW CTTSNHEASK CASFK---KA IPA-DG--PL M--ACVRRTS PQECIRAISD

NP_598738_Mus_musculus V--PDKTVKW CAVSEHENTK CISFRDHMKT VLPPDG--PR L--ACVKKTS YPDCIKAISA

XP_003789550_Otolemur_garnettii V--PEKTVRW CAVSDHEASK CHGFRDNMKK VLP-DG--VR V--SCVKKAS HLDCIKAIAA

ICA subfamily

XP_005600996_Equus_caballus V--SVKTVRW CTVSTHEASK CSRFSQNMRR VVPADG--PH V--ICVKRTS YLECIKAIAA

XP_005202014_Bos_taurus V--PERTVRW CTVSNLEASK CYSFHDNMKK VLSVES--PH V--TCVKRTS YLDCIRAIAA

XP_005675609_Capra_hircus V--PEKTVRW CTVSDLEASK CYSFHDNMKK VLSVDG--PH V--ACVKRTS YRDCIRAIAA

XP_004419416_Ceratotherium_simum_simum V--PEQNVRW CTVSNHEARK CSSFHNYMKS VLPVG---PH I--TCVQRAS CLECIKAIAA

XP_004603285_Sorex_araneus A--PEQTVRW CTTSDHEASK CSSW----KK ALSAGG--PH M--DCVRRAS AQECIKSISA

XP_005634506_Canis_lupus_familiaris V--PGKTVRW CTISNHEARK CSSFSDNMKR VLPEDG--PH V--TCVKRTS HLECIKAITA

NP_082194_Mus_musculus L--PEKTIRW CVVSDHEATK CSSFRDNMKK VLPAGG--PA V--TCVRKMS HPECIRDISA

L subfamily

NP_001157446_Equus_caballus AP-RK-SVRW CTISPAEAAK CAKFQRNMKK VRG-----PS V--SCIRKTS SFECIQAIAA

XP_541903_Canis_lupus_familiaris AP-RK-NVRW CTTSKAEAKK CSKFQVNMKK VGG-----PI V--SCTRKAS RQECIQAIKA

NP_002334_Homo_sapiens G--RRRSVQW CAVSQPEATK CFQWQRNMRK VRG-----PP V--SCIKRDS PIQCIQAIAE

XP_004419670_Ceratotherium_simum_simum AP-KR-RVRW CTISPAEVAK CIKFQRNIKK VRG-----PS V--HCIKKTS RFECIQAIAA

NP_001272477_Capra_hircus AP-RK-NVRW CAISLPEWSK CYQWQRRMRK LGA-----PS I--TCVRRTS ALECIRAIAG

XP_004614649_Sorex_araneus IP-KK-SVRW CTTSSLEGVK CDKFQSQMKI VSG-----LL L--TCVKKNS YHQCIQAIAE

NP_001179241_Bos_taurus G----MEVRW CTISDPEQQK CSDMSKAFQQ AGIQPS---- V--LCVQGTS ADHCVQLITA

NP_032548_Mus_musculus KA-T--TVQW CAVSNSEEEK CLRWQNEMRK VGG-----PP L--SCVKKSS TRQCIQAIVT

XP_003476935_Cavia_porcellus IP-KG-NVRW CAISSDEARK CYNLQKNMKR VGG-----PP V--SCIKKDS YRDCLQAISE

NP_851341_Bos_taurus AP-RK-NVRW CTISQPEWFK CRRWQWRMKK LGA-----PS I--TCVRRAF ALECIRAIAE

M subfamily *

XP_002937877_Xenopus_tropicalis L----NEVRW CTISDPENKK CNDMKAAFGQ AKITPM---- L--SCVNGKS GMDCARMIMN

XP_005989453_Latimeria_chalumnae M----SQVRW CTISVLELNK CNDMKEAFRG ANIHPL---- L--SCVEGTG KMDCVQKIAD

XP_005295722_Chrysemys_picta_bellii L----QHVRW CTISEQEQAK CNDMSAAFTD ANILPN---- V--LCVRGGS AINCTHMIKN

XP_001512738_Ornithorhynchus_anatinus TTLGLSEVRW CTTSESEQEK CEDMSTAFKE AGIHPL---- L--TCVQEAS ADDCVRLIAA

XP_005675137_Capra_hircus G----MEVRW CTISDPEQQK CSDMSKAFQQ AGIQPS---- V--LCVQGTS ADHCVQLITA

XP_001381165_Monodelphis_domestica ----MSELRW CTVSDPEQQK CLAMAEAFKE AGIRPA---- L--GCVQETP SEPCIHLIEV

XP_006030068_Alligator_sinensis L----PHIRW CTISVEEMAK CNDMNSAFAE ANILPR---- L--SCVRGGS ASNCTYLIKN

NP_038928_Mus_musculus V----MEVQW CTISDAEQQK CKDMSEAFQG AGIRPS---- L--LCVQGNS ADHCVQLIKE

XP_005147173_Melopsittacus_undulatus L----EHIQW CTVSKQELSK CNDMSKAFGR AGILPP---- L--ECTEGGS AANCTQMIKD

XP_004424596_Ceratotherium_simum_simum G----MEVRW CTTSDPEQQK CSDMSKAFQE AGIRPS---- L--LCIQGAS ADHCVQLITA

XP_004089765_Nomascus_leucogenys G----MEVRW CATSDPEQQK CSNMSKAFRE AGIQPS---- L--LCVQGTS ADHCIQLIAA

XP_002189009_Taeniopygia_guttata L----ERVRW CTVSEPELSK CNGMSKAFSE AGILPP---- L--ECTAGGS AANCTQMIKD

XP_005489492_Zonotrichia_albicollis L----ERVRW CTVSDPELSK CNDMSKAFSE AGILPP---- L--ECAAGGS AANCTQMIKD

XP_005601955_Equus_caballus G----MEVRW CTTSDPEQQK CSDMSKAFQD AGIQPA---- L--LCVQGAS ADHCVQLITA

NP_005920_Homo_sapiens G----MEVRW CATSDPEQHK CGNMSEAFRE AGIQPS---- L--LCVRGTS ADHCVQLIAA

NP_990538_Gallus_gallus L----ERVRW CTMSNQELSK CKDMSNAFTG AGILPP---- L--ECMEGES AANCTQMIKD

XP_003209160_Meleagris_gallopavo L----ERVRW CTVSNQELSK CKDMSNAFAG AGILPP---- L--ECMEGES AANCTQMIKN

XP_005051116_Ficedula_albicollis L----ERVRW CTVSEQELSK CNDMSKAFSE AGILPP---- L--ECTAGGS AANCTQMIKD

XP_003477010_Cavia_porcellus SGEQVTEVRW CTISDAEQLK CQDMSKAFRE AGIQPI---- I--ACVQGTS AGHCMQLISA

EMP29367_Chelonia_mydas L----QHVRW CTVSEQEQAK CNDMRAAFTD ANIFPN---- V--LCVNGGS AINCTHMIKN

XP_004603294_Sorex_araneus S----VEVRW CTTSEAEQQK CSDMSQAFLE AGLEPP---- L--ECVQATS ADHCVQLIAA

XP_005457210_Oreochromis_niloticus Q----STIKW CTISQNEQRK CQAMSQAFST ASIRPS---- L--SCVNAAS VEDCGQKLQR

XP_003974413_Takifugu_rubripes Q----SSIRW CTISDAEHRK CEAMSQALAE ASIRPS---- V--SCVSGVT VEGCVQKLEK

XP_004562160_Maylandia_zebra Q----STIKW CTISQNEQRK CQAMSQAFST ASIRPS---- L--SCVNAAS VEDCGQKLQR

XP_003792792_Otolemur_garnettii G----MEVRW CTISEPEQQK CSDMSKAFQE AGIQPS---- L--LCVQGSS ADHCIQLITA

M-like subfamily **

XP_005810510_Xiphophorus_maculatus ------MRW CTVSDPEQRK CAELAKALVA VLPPAAVAAF ARLSCVRASN TADCIDKIKA

XP_004086212_Oryzias_latipes ------MRW CTVSSPEQKK CAELAKALVA VLPPAAVADF ARLSCIRASS TGDCMDRIKA

XP_005493436_Zonotrichia_albicollis -----KKFRW CTLSDLEQRK CAELSKVLTA VLPLTPASSF TRISCIRAHN TYDCIDKIRA

XP_003973359_Takifugu_rubripes ---RPKKIRW CTVSEPEQRK CAELAKSLVT VLPPAAVAAF ARVSCVRASS TMDCIDKIRA

XP_003448463_Oreochromis_niloticus ----AKKMRW CTVSDPEQRK CAELAKALAA VVSPAILAAF ARLSCIRAYS TTDCINRIKA

XP_004548831_Maylandia_zebra ----AKKMRW CAVSDPEQRK CAELAKALAA VVSPATLAAF ARLSCIRASS TTDCINRIKA

XP_005147853_Melopsittacus_undulatus -----KKFRW CTLSDLEQRK CAELSKALMA VVPLAAVNSF ARISCIRAHN TYDCIDKIRV

EMP34150_Chelonia_mydas -----KKFRW CTLSDLEQRK CAELSKVLLA VLPLATINSF ARVSCIRAQN THDCIDKIRV

XP_002931966_Xenopus_tropicalis -----KKFRW CTLSDLEQRK CSHLARTLQT ILPAS--HAF SRVSCVRAHT TQDCMSKIRG

Other

XP_006008475_Latimeria_chalumnae DS--ETKITW CTKSRNEYLK CQELKSAMAS VYPNFF------CIEKTN TFDCIKAIEN

XP_001364584_Monodelphis_domestica T--TEQQVRW CVISENELKK CNELKEALKT VNV-----PN L--ACIKKTS HMDCIKAIAE

NP_001027487_Xenopus_tropicalis MPPKEKQVRW CAKSKSEFSK CRDLVNTCKN KEITLS------CVEKSS TDECLTAIQN

XP_003763732_Sarcophilus_harrisii T--AEQQVRW CVQSENEQKK CNELKEVLKG TTS-----PS L--TCVKKIS YKDCFRAIWK

NP_001083070_Xenopus_laevis MP-KEKQVRW CVKSKSELNK CRDLVNTCKN KEITLS------CVEKPN TDECLTAIQE

XP_005796062_Xiphophorus_maculatus AAP-AAKVRW CVKSDQELRK CTDLAAAAPE FS------CVKKES TLECIIAIKA

XP_004542735_Maylandia_zebra ARPGSQKVKW CVKSDQELRK CLDLAAAAPA FS------CVKKEN TLECIIAIKA

XP_003978228_Takifugu_rubripes AVP-AARIRW CLKSEAEYLK CKRLELVAPA IS------CVRRES TMECIVAITA

NP_001116384_Oryzias_latipes AVP-AQKVKW CVKSDQEFRK CSDLAAASPA FS------CVKKES TLDCIIAIKA

NP_001079812_Xenopus_laevis IQ-KEKQVRW CVKSNSELKK CKDLVDTCKN KEIKLS------CVEKSN TDECSLLFRK

O subfamily 120

NP_990635_Gallus_gallus NEADAISLDG GQVFEAGLAP YKLKPIAAEI YEHT--EGST TSYYAVAVVK KG-TEFTVND

XP_003209092_Meleagris_gallopavo NEADAISLDG GQVFEAGLAP YKLKPIAAEV YEHT--EGST TSYYAVAVVK KG-TEFTVND

XP_005143141_Melopsittacus_undulatus NEADAISLDG GQVFEAGLAP YKLKPIVAEV YTQN--GGST TSYYAVAVAK KG-TGFTIND

XP_005489599_Zonotrichia_albicollis SEADAISLDG GQVFEAGLAP YKLKPIAAEV YEHS--EGST TSYYAVAVVK KG-TGFSIDE

XP_002192726_Taeniopygia_guttata SEADAISLDG GQVFEAGLAP YKLKPIAAEV YEHS--EGST TSYYSVAVAK KG-TGFSIDE

BAJ23165_Dromaius_novaehollandiae NETDAISLDG GQIFEAGLGP YKLKPIAAEV YEGT--QGST TYYYAVAVVK KG-TDFTIND

CAH10347_Struthio_camelus NETDAITLDG GHIFEAGLGP YNLKPIVAEV YEGS--QGPT ASYYAVAVVK KG-TGFTIND

XP_005050798_Ficedula_albicollis SEADAISLDG GQIFEAGLAP YKLKPIAAEV YEHS--EGST TSYYAVAVVK KG-TGFSIDG

XP_005303689_Chrysemys_picta_bellii NEADAISLDG GHIFEAHLAP YNLKPVVAEV HGTG--KDAA TSYYAVAVVK KG-TGFTIRE

XP_006037143_Alligator_sinensis KEADAISLDG GHIFEAGIEQ DKL--IVAEV HGVG--EEST TNYYAVAVVK KG-SGFTINE

CAK18228_Lacerta_agilis SEADAVSLGG GLIYDAGLAP HNLKPIVAEV YGTSTGGESV TSYYAVAVVK KN-TVQSLAE

CAK18230_Crocodylus_niloticus NQADAISLDA GHIFEAGIDP YKLKPIVAEV HEDE--GALT TSYYAVAVVK KG-SGFTIHQ

CAK18222_Chamaeleo_chamaeleon SEADAASVDG GLVYDAALHP NNLKPIVAEV YATS-EGESA TSYRAVAVVK KG-TGFFLSG

XP_003218456_Anolis_carolinensis NEADAITLDG GHVFDAGLAP YNLKPIVSEF YFS--LSETV TSYRAVAVVK KG-MVESLAD

CAK18224_Python_bivittatus SEADAITLDA GLIAEAGLSP YNLKPIVAEV HKKG-SEETT TSYYAIAVVK KG-TISSLSE

CAK18223_Boaedon_fuliginosus SEADAITLDA GLILEASLNP YYLKPVVAEL HQKG-SGVTT TSYHAIAVVK KG-TITSLED

CAK18221_Natrix_natrix SEADAITLDA GLILEASLNP YYLKPVIAEL HPKG-SKVTT TSYHAIAVVK KG-TITSLED

S subfamily

XP_005600539_Equus_caballus NEADAVTLDA GLVFEAGLSP YNLKPVVAEF YGSK--TEPQ THYYAVAVVK KN-SNFQLNQ

XP_534268_Canis_lupus_familiaris NEADAVTLDA GLVFEAGLNP YNLKPVVAEF YGK----DKQ TRYYAVAVVK KS-STFNLNQ

XP_003265287_Nomascus_leucogenys NEADAVTLDA GLVYDAYLAP NNLKPVVAEF YGSK--EDPQ TFYYAVAVVK KD-SGFQMNQ

XP_005675608_Capra_hircus NEADAVTLDG GLVYEAGLKP NNLKPVVAEF HGTK--DNPQ THYYAVAVAK KG-TDFKLNE

NP_001054_Homo_sapiens NEADAVTLDA GLVYDAYLAP NNLKPVVAEF YGSK--EDPQ TFYYAVAVVK KD-SGFQMNQ

NP_803450_Bos_taurus NEADAVTLDG GLVYEAGLKP NNLKPVVAEF HGTK--DNPQ THYYAVAVVK KD-TDFKLNE

XP_003476776_Cavia_porcellus NEADAVTVDA GLVHEAGLAP KNLKPVVAEF YGSQ--ENPQ TFYYAVAVVK KG-TDFQLNQ

XP_004419415_Ceratotherium_simum_simum NEADAVTLDA GLVFEAGLAP YNLKPVVAEY HKSE--DDKQ TRYYAVAVVK KT-SNFQLNQ

XP_004603284_Sorex_araneus GEADAVTLDA GLVFEAGLAP YNLKPVVAEY YGSE--ADKQ THYYAVAVVK KT-SNFQFNE

NP_598738_Mus_musculus SEADAMTLDG GWVYDAGLTP NNLKPVAAEF YGSV--EHPQ TYYYAVAVVK KG-TDFQLNQ

XP_003789550_Otolemur_garnettii NEADAVTLDA GWVYEAGLTP NNLKPVVAEV YGSK--DNPL TYYYAVAVVK KG-SGFQWNE

ICA subfamily

XP_005600996_Equus_caballus KEADAVTIDG GLVFEAGLAP YNLKPIVAEF YGSK--RDPQ THHYVVALVK KA-SDFQLNQ

XP_005202014_Bos_taurus HEADAVMVDG GLVYEAGLKP YNLKPVVAEF YGSK--DDPQ THHYVVAVVK KG-SNFQLNQ

XP_005675609_Capra_hircus HEADAVMVDG GLVYEAGLKP YNLKPVVAEF YGSK--DDPQ THYYVVAVVK KG-SDFQLNQ

XP_004419416_Ceratotherium_simum_simum KEADAVTVDG GLVFEAGLAP YNLKPIVAEF YWSE--DGPQ THYYVVAVVR RG-SDFQLSQ

XP_004603285_Sorex_araneus NEADAVTVEG GLVLEAGLAP YHLKPVAAEV YGSR--DDPQ THHYAVALVK KG-SGFQLNQ

XP_005634506_Canis_lupus_familiaris NEADAVTVEA GLVFEAGLPP FNLKPIVAEF HGSK--NNPQ TVHYAVAVVK KD-SNIQLKG

NP_082194_Mus_musculus NKVDAVTVDG ALVAEADLPH HSLKPIMAEY YGSK--DDPK THYYVVAMAK KG-TGFQLNQ

L subfamily

NP_001157446_Equus_caballus NKADAVTLDG GLVYEAGLHP YKLRPVAAEV YQTR--GKPQ TRYYAVAVVK KG-SGFQLNQ

XP_541903_Canis_lupus_familiaris NKADAVTLDG GLVFEAGLEP NKLRPIAAEV YGTQ--TKQQ IHYYAVAIAK KG-TNFQLNQ

NP_002334_Homo_sapiens NRADAVTLDG GFIYEAGLAP YKLRPVAAEV YGTE--RQPR THYYAVAVVK KG-GSFQLNE

XP_004419670_Ceratotherium_simum_simum NKADAVTLDG GLVYDAGLEP YKLRPIAAEV YETG--GKPQ THYYAVAIVK KG-SGFQLSQ

NP_001272477_Capra_hircus KNADAVTLDS GMVFEAGRDP YKLRPVAAEI YGTE--KSPQ THYYAVAVVK KG-SNFKLDQ

XP_004614649_Sorex_araneus NKADAVTLGS SLIYEAGQEP YNLRPVVAEV YGTE--AEPQ THYYAVAVAK KG-SNVQLNN

NP_001179241_Bos_taurus QEADAITLDG GAIYQAG-KE HGLKPVVGEV YDQE----IG TSYYAVAVVK RG-SQVTINT

NP_032548_Mus_musculus NRADAMTLDG GTMFDAGKPP YKLRPVAAEV YGTK--EQPR THYYAVAVVK NS-SNFHLNQ

XP_003476935_Cavia_porcellus NKADAITLDG GQLYEAALDP YNLRPVAAEV YGSE--ANPR THYYAVAVAK RS-RNFRLDQ

NP_851341_Bos_taurus KKADAVTLDG GMVFEAGRDP YKLRPVAAEI YGTK--ESPQ THYYAVAVVK KG-SNFQLDQ

M subfamily

XP_002937877_Xenopus_tropicalis NEADAVTLNG ELIYQAG-SQ YNLKPVAGEV YDQG----MG TSYYAVAVVR KN-SSYTINN

XP_005989453_Latimeria_chalumnae NLADAVTLDG GYIYQAG-KE YDLKPVVGEK YDQD----VG TSYYAVAVVS SSNTTININN

XP_005295722_Chrysemys_picta_bellii NLADAVTLDG GTIYQAG-KE HNLKPVVGEV YDQE----IG TSYYAVAVVR KN-SFISINS

XP_001512738_Ornithorhynchus_anatinus NKADAITLDG GAIYEAG-KE YNLKPVVGEV YDQE----VG TSYYAVAVVR RN-SSLDINS

XP_005675137_Capra_hircus QEADAITLDG GAIYQAG-KE HGLKPVVGEV YDQE----IG TSYYAVAVVK RG-SQVTVNT

XP_001381165_Monodelphis_domestica NKADAVTLDG GAIYEAG-KY HGLKPVVGEM YDQE----AA TSYYAVAVVR RG-SSLTINH

XP_006030068_Alligator_sinensis NMADAVTLDG GSIYQAG-KE YNLKPVVGEV YDQE----MG TSYYAVAVTR KD-SFITINS

NP_038928_Mus_musculus QKADAITLDG GAIYEAG-KE HGLKPVVGEV YDQD----IG TSYYAVAVVR RN-SNVTINT

XP_005147173_Melopsittacus_undulatus HLADAVTLDG HLIYQAG-KE HGLKPVVGEV YDQE----IG TSYYAVAVVR KS-SNITINS

XP_004424596_Ceratotherium_simum_simum QEADAITLDG GAIYEAG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVK RS-SHVTIDT

XP_004089765_Nomascus_leucogenys QEADAITLDG GAIYEAG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVK RS-SRVTIDT

XP_002189009_Taeniopygia_guttata DLADAVTLDG RLIFQAG-RE HGLKPVVGEV YDQE----IG TSYYAVAVVK RS-SNITIKS

XP_005489492_Zonotrichia_albicollis DLADAVTLDG RWIFQAG-RE HGLKPVVGEV YDQE----IG TSYYAVAVVK KS-SNISMGS

XP_005601955_Equus_caballus QEADAITLDG GAIYEAG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVK RS-SHVTINS

NP_005920_Homo_sapiens QEADAITLDG GAIYEAG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVR RS-SHVTIDT

NP_990538_Gallus_gallus YLADTVTLDG RWIYQAG-KE YGLKPVVGEV YDQE----IG TSYYAVAVVR KG-SNITINS

XP_003209160_Meleagris_gallopavo YLADAVTLDG RWIYQAG-KE YGLKPVVGEV YDQE----IG TSYYAVAVVR KG-SNITINS

XP_005051116_Ficedula_albicollis DLADAVTLNG RLIFQAG-RE HGLKPVVGEV YDQE----IG TSYYAVAVVK RS-SNITINS

XP_003477010_Cavia_porcellus QKADAVTLDG EAIYEAG-KE HGLKPVAGEL YDGE----VG TAYYAVAVVR KN-FSVTINT

EMP29367_Chelonia_mydas NLADAVTLDG GTIYQAG-KE HNLKPVVGEV YDQE----IG TSYYAVAVVR KK-SFISINS

XP_004603294_Sorex_araneus EEADAITLDG GAIYEAG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVK RG-SNLTLST

XP_005457210_Oreochromis_niloticus KEADALSMSA KDIYNLG-KT VSLKMAGSES Q-AG----SV TTYYAVAVVK KENSGININN

XP_003974413_Takifugu_rubripes KAADAFSMFG SDIYKHG-KM NSIQVAASES KSDG----TG ASYYAVAVVK KANQGITVKN

XP_004562160_Maylandia_zebra KEADALSMSA KDIYNLG-KT ASFKMAGSES Q-AG----SV TTYHAVAVVT KKNSGINIHN

XP_003792792_Otolemur_garnettii QEADAITLDG GAIYEGG-KE HGLKPVVGEV YDQE----VG TSYYAVAVVK RS-SHVTINT

M-like subfamily * ** *

XP_005810510_Xiphophorus_maculatus NRADIVALDA GEVYSAV-KQ FDLVVVAKEV YTDG------ACILSVAVVT NR-S-LDIRS

XP_004086212_Oryzias_latipes NRADIVSLDA GEVYSAV-KQ FDLVTVAKEI YSEG------GCVLSVAVVR NS-S-LAVQS

XP_005493436_Zonotrichia_albicollis NKADAASLDA GDVYSAV-KL YGLAVVAKEI YDQG------NCVFAVAVAK RG-A-LDVRR

XP_003973359_Takifugu_rubripes NRADIVTLDA GEVYSAV-KQ FDLVTVAKEI YSDG------GCILSVAVVK NS-S-LDISS

XP_003448463_Oreochromis_niloticus NRADIVTLDA GEIYSAV-KQ FDLVAIAKEI YSDG------GCTLSVAVVR NN-S-LDIRS

XP_004548831_Maylandia_zebra NRADIVTLDA GEIYSAV-KQ FDLVAIAKEI YSDG------GCTLSVAVVR NS-S-LDIRS

XP_005147853_Melopsittacus_undulatus NKADAASLDA GDVYSAV-KL YGLTVVAKEI YDQG------NCVFAVAIAK RG-T-LDIQR

EMP34150_Chelonia_mydas NKADAVSLDA GDVYSAV-KL YGLTVVAKEI YEEG------NCVFAVAIAR RG-T-LDIQR

XP_002931966_Xenopus_tropicalis NKADAVSLDA GDVYTAI-KQ YDLAVVAKER HSDG------SCVYAVAVAR RG-T-LNIHK

Other

XP_006008475_Latimeria_chalumnae KEADAISVDG GDIYRAGLMP HNLRPIIAEK YG----EDDG TCYFAVAVAK KG-SGFSFKE

XP_001364584_Monodelphis_domestica NEADAQTVDG GLIYEGGLVP YNLKPIVAED YGTE--SSPL SQYFAVAVVK KG-TGFQFRD

NP_001027487_Xenopus_tropicalis DRADAICVDG GDVYKGSLQP YNLKPIMAEN YGSH--TEPD TCYYAVAVVK KS-STFTFDE

XP_003763732_Sarcophilus_harrisii NEADAISVDG GLVYEAVAAP FGLKPIIAED YGSE--KEQQ IYSFAVAVVK KG-TDFQLKD

NP_001083070_Xenopus_laevis DRADAICVDG GDVYKGSLQP YNLKPIMAEN YGSQ--TETD TCYYAVAVVK KS-STFTFDE

XP_005796062_Xiphophorus_maculatus SEADAITVDG GDVYTAGLNN YDLHPILAED YGET----SD TCYYAVAVVK KG-TNFGIRD

XP_004542735_Maylandia_zebra GEADAITVDG GDIYTAGLNN YDLHPIIAED YGAG----TD TCYYAVAVAK KN-TGFGFRD

XP_003978228_Takifugu_rubripes KLADAITLDG GDVYTAGLKN YDLHPIIAED YGPS----SD TCYYAVAVVK KG-SSFGIKD

NP_001116384_Oryzias_latipes GEADAITVDG GDVYTAGLNN YDLHPIIAED YGTS----SE TCYYAVAVAK KG-TTFGIRD

NP_001079812_Xenopus_laevis TMQMQFVWTG GDVYKGSLQP YNLKPIMAEN YGSH--TETD TCYYAVAVVK KS-SKFTFDE

O subfamily 180

NP_990635_Gallus_gallus LQGKNSCHTG LGRSAGWNIP IGTLLHWGAI EWEGIESGSV EQAVAKFFSA SCVPGATI--

XP_003209092_Meleagris_gallopavo LQGKTSCHTG LGRSAGWNIP IGTLIRRGAI EWEGIDSGSV EQAVAKFFSA SCVPGATT--

XP_005143141_Melopsittacus_undulatus LQGKTSCHTG LGRSAGWNIP IGTLLRRGDI KWEGKDSGSI EQAVANFFSA SCVPGATT--

XP_005489599_Zonotrichia_albicollis LQGKTSCHTG LGRSAGWVIP IGTLIHRGAI EWDGKDSGSI EQAVANFFSA SCVPGATT--

XP_002192726_Taeniopygia_guttata LRGKTSCHTG LGRSAGWVIP IGTLIHRGAI EWDGKDSGSI EQAVANFFSA SCVPGATT--

BAJ23165_Dromaius_novaehollandiae LKGKTSCHTG LGRSAGWNIP IGTLIRRGDI PWDGIDSGSL EQEVAKFFSA SCVPGAN---

CAH10347_Struthio_camelus LKGRTSCHSG LGRSAGWHIP IGTLVRRGDI KWDGIESGSI EQEVAKFFSA SCVPGAT---

XP_005050798_Ficedula_albicollis LQGKTSCHTG LGRSAGWVIP IGTLIRRGDI EWDGKESGSI EQAVANFFSA SCVPGATT--

XP_005303689_Chrysemys_picta_bellii LKGKKSCHTG LDRSAGWVIP IGTLLYHQTL SW--DRDTPI THAVAQFFSA SCVPGAPAN-

XP_006037143_Alligator_sinensis LRGKKSCHTG LKRAAGWVIP IGTLVDKGII DR-GPGSDPI EKDVAGFFSA SCVPGATTE-

CAK18228_Lacerta_agilis LRGKKSCHTG LGRSAGWVMP VGRLLSLGLI EWAGAETEPI EKAVAKFFSA SCVPGCKN--

CAK18230_Crocodylus_niloticus LQGKKSCHTG LKKSAGWVIP IGTLVKKGII VR-GPGSDSV EKAVAKFFSA SCVPGATTE-

CAK18222_Chamaeleo_chamaeleon LKGRKSCHTG LHRSAGWVIP IGTLLAQKIL KWEGPSTEQL EKAVASFFSA SCVPGAS---

XP_003218456_Anolis_carolinensis LRGKKSCHTG LGRSAGWNIP IGSMVAKQYI DWKGADTEPL EKAVARFFSA SCVPGAS---

CAK18224_Python_bivittatus LKNKKSCHTG FDRSAGWNIP IGTLLRVGLI KWEGVEVEPV QLAVARYFSV SCVPGVKD--

CAK18223_Boaedon_fuliginosus LRGKRSCHTG FRRSAGWNIP VGTLLSKNLL QWDGTETEPV EKAVGRFFAA SCVPGVKN--

CAK18221_Natrix_natrix LRGKKSCHTG FRRSAGWNIP VGTLLSKNLL QWDGTESEPV EIAVGRFFSA GCVPGLKN--

S subfamily

XP_005600539_Equus_caballus LQGKKSCHTG LGRSAGWNIP IGLLYWQ--L PEPRES---L QKAVSNFFAG SCVPCADRTA

XP_534268_Canis_lupus_familiaris LQGKKSCHTG LGRSAGWNIP MGLLYWK--L PEPRES---L QKAASSFFTA SCVPCVDRTA

XP_003265287_Nomascus_leucogenys LRGKKSCHTG LGRSAGWNIP IGLLYCD--L PEPRKP---L EKAVANFFSG SCVPCADGTD

XP_005675608_Capra_hircus LKGKKSCHTG LGRSAGWNIP MGRLYKE--L PDPQES---I QRAAASFFSA SCVPCADQSS

NP_001054_Homo_sapiens LRGKKSCHTG LGRSAGWNIP IGLLYCD--L PEPRKP---L EKAVANFFSG SCAPCADGTD

NP_803450_Bos_taurus LRGKKSCHTG LGRSAGWNIP MGKLYKE--L PDPQES---I QRAAANFFSA SCVPCADQSS

XP_003476776_Cavia_porcellus LQGKKSCHTG LGRSAGWVIP IGSIFCD--L PEPRKP---L EKAVASFFSG SCVPCADAAA

XP_004419415_Ceratotherium_simum_simum LQGKKSCHTG LGRSAGWNIP IGLLYWQ--L PEPRDS---V PKAVANFFAG SCVPCADRTA

XP_004603284_Sorex_araneus LQGKKSCHTG LGRSAGWNIP VGLLYWQ--L PQPHDS---L QKAVASFFGG SCVPCADGKS

NP_598738_Mus_musculus LEGKKSCHTG LGRSAGWVIP IGLLFCK--L SEPRSP---L EKAVSSFFSG SCVPCADPVA

XP_003789550_Otolemur_garnettii LRGKKSCHTG LGRSAGWNIP IGLLYCD--L PEPRQP---L ERAVANFFSG SCVPCADGDS

ICA subfamily

XP_005600996_Equus_caballus LQGKKSCHTG LGRSAGWNIP IGLLLPS--D PFQEA------VAKFFSS SCVPCVDGKE

XP_005202014_Bos_taurus LQGKKSCHTG LGWSAGWNIP MRMLLPS--D WSQKA------AAKFFAG SCVPCADQSN

XP_005675609_Capra_hircus LQGKKSCHTG LGWSAGWNIP MRLLLPS--N WSQEA------VAKFFAS SCVPCADQSN

XP_004419416_Ceratotherium_simum_simum LQGKKSCHTG LRCSAGWKIP MGMLLPS--D PVEEA------VAKFFSS SCVPCADGKE

XP_004603285_Sorex_araneus LQGRKSCHPG LGWAAGWHIP VRTLLPS--G SGEEA------VAQFFSG SCVPCADEKN

XP_005634506_Canis_lupus_familiaris LQGKKSCHPG LGWSAGWNIP IRIFFPS--V SVEEAAT--- -AEMAQFFVG SCVPCANRME

NP_082194_Mus_musculus LRGKKSCHTG LGWSAGWYVP LSTLLPS--G SRET------AAATFFSS SCVPCADGKM

L subfamily *

NP_001157446_Equus_caballus LQGVKSCHTG LGRSAGWNIP IGTLRPY--L NWTGPPEP-L QKAVANFFSA SCVPCADGKQ

XP_541903_Canis_lupus_familiaris LQGVRSCHTG LGRSAGWNIP IGTLRPF--L NWTGPPEP-L EEAVAKFFSA SCVPCADGKQ

NP_002334_Homo_sapiens LQGLKSCHTG LRRTAGWNVP IGTLRPF--L NWTGPPEP-I EAAVARFFSA SCVPGADKGQ

XP_004419670_Ceratotherium_simum_simum TRGVKSCHTG LGRSAGWNIP VGILRPY--L NWTGPPEP-L EKAVANFFSA SCVPCADGTQ

NP_001272477_Capra_hircus LQGQKSCHMG LGRSAGWNIP VGILRPP--L SWTESAEP-L QGAVARFFSA SCVPCVDGKA

XP_004614649_Sorex_araneus LQGVKSCHPG LNWTAGWTVP VGVLRPV--L NWNGSPKS-L QDAVAGFFSA SCVPCVNRKQ

NP_001179241_Bos_taurus LKGMKSCHTG INRTVGWNVP VGYLVESGRL SVMGCDVLR- --AVSDYFGG SCVPGAGETS

NP_032548_Mus_musculus LQGLRSCHTG IGRSAGWKIP IGTLRPY--L NWNGPPAS-L EEAVSKFFSK SCVPGAQKDR

XP_003476935_Cavia_porcellus LQGLRSCHTG INRTAGWRIP TGVLRPF--L NWRGPPEP-I EAAVAKFFSA SCVPGVDGVR

NP_851341_Bos_taurus LQGRKSCHTG LGRSAGWIIP MGILRPY--L SWTESLEP-L QGAVAKFFSA SCVPCIDRQA

M subfamily

XP_002937877_Xenopus_tropicalis LKGAKSCHTG FQRTAGWNVP IGYLIDSGRI AVVACNIQK- --AVSSFFSQ SCVPGSTQA-

XP_005989453_Latimeria_chalumnae LRGKKSCHTG LNRTVGWNVP IGYLIDSGRM SVMDCNVSR- --AVSEFFSA SCVPGANIPG

XP_005295722_Chrysemys_picta_bellii LKGVKSCHTG INRTVGWNVP VGYLIDSGRM SVMGCSVPK- --AVSEYFSA SCVPGANNVN

XP_001512738_Ornithorhynchus_anatinus LRGTKSCHTG INRTVGWNVP VGYLVESGRL SVMGCDVPR- --AVSEYFSS SCVPGAGETN

XP_005675137_Capra_hircus LKGMKSCHTG INRTVGWNVP VGYLVDSGRL SVMGCDVLR- --AVSDYFGG SCVPGAGETS

XP_001381165_Monodelphis_domestica LRGARSCHTG LNRTAGWNVP VGYLVESGRL AVMGCDVLR- --AVSSYFGG SCVPGAAESG

XP_006030068_Alligator_sinensis LEGARSCHTG INRTVGWNVP VGYLIDSGRL AVMGCNIPT- --AVSEYFNA SCVPGANAAN

NP_038928_Mus_musculus LKGVKSCHTG INRTVGWNVP VGYLVESGHL SVMGCDVLK- --AVGDYFGG SCVPGTGETS

XP_005147173_Melopsittacus_undulatus LKGARSCHTG INRTAGWDVP VGYLIDSGRL AAMGCDLPT- --AVSDYFSA SCVPGANGAN

XP_004424596_Ceratotherium_simum_simum LKGVKSCHTG INRTVGWNVP VGYLVESGRL SVMGCNVLK- --AVSDYFGG SCVPGAGETS

XP_004089765_Nomascus_leucogenys LKGVKSCHTG INRTVGWNVP VGYLVESGRL SVMGCDVLK- --AVSDYFGG SCVPGAGETG

XP_002189009_Taeniopygia_guttata LKGARSCHTG INRTAGWDVP VGYLTDAGHL AAMGCDLPK- --AVSDYFNA SCIPGANGVN

XP_005489492_Zonotrichia_albicollis LKGARSCHTG INRTAGWDVP VGHLTATGHL APMGCDLPQ- --AVSDYFNA SCVPGANGVN

XP_005601955_Equus_caballus LKGVKSCHTG INRTVGWNVP IGYLVDSGRL SVMGCDVLK- --AVSDYFGS SCVPGAGETS

NP_005920_Homo_sapiens LKGVKSCHTG INRTVGWNVP VGYLVESGRL SVMGCDVLK- --AVSDYFGG SCVPGAGETS

NP_990538_Gallus_gallus LKGVRSCHTG INRTAGWNVP VGYLIDSGRL PAMGCDLPK- --AVSDYFSA SCVPGTNSAS

XP_003209160_Meleagris_gallopavo LKGIRSCHTG INRTAGWNVP VGYLIDSGRL PAMACDLPK- --AVSDYFSA SCIPGANSAN

XP_005051116_Ficedula_albicollis LKGVRSCHTG INRTAGWDVP VGYLTDIGHL AAMGCDLPK- --AVSDYFNA SCVPGANGVN

XP_003477010_Cavia_porcellus LKGVKSCHTG LQQTAGWNVP VGFLVETGRL SVMGCDVLT- --AVSEFFGG SCVPGAGETN

EMP29367_Chelonia_mydas LKGVRSCHTG INRTVGWNVP VGYLIDSGRM SVMGCNVPK- --AVSEYFNA SCVPGANNVN

XP_004603294_Sorex_araneus LKGARSCHTG INRTVGWNVP VGFLVDSGHL SVMGCDVLKG -EAPRTWYRP ASLRGSGSAK

XP_005457210_Oreochromis_niloticus LAGKKSCHTG IGRTVGWNMP IGYLIDQGYM SVMGCNIPQ- --GVANFFSA SCIPGAT-QG

XP_003974413_Takifugu_rubripes LAGKKSCHTG KGRTAGWNMP IGYLMDQGYM SVMGCNIPE- --GVANFFSA SCVPGATAQG

XP_004562160_Maylandia_zebra LAGKKSCHTR IGRTAGWNMP IGYLIDQGYM SVMGCNIPQ- --GVANFFNA SCIPGAT-QG

XP_003792792_Otolemur_garnettii LKGVKSCHTG INRTVGWNVP VGYLVESGRL SVMGCDVLK- --AVSDYFGG SCVPGAGETS

M-like subfamily * * * * *

XP_005810510_Xiphophorus_maculatus LQGLRSCHSG ARWTAGWSLP LGFLLSRNYL TWSKENPLS- -QDVGAFFRA SCVPGAATMA

XP_004086212_Oryzias_latipes LQGLRSCHSG VRWTAGWSIP LGFLLSRNYL SWSKDHPLS- -QEVGGFFSA SCCPGAAAMS

XP_005493436_Zonotrichia_albicollis LRGVRSCHNG AKWTSGWNIP LGFLLARNSL SWDESQPLS- -QAVSEYFNA SCIPGVGVAA

XP_003973359_Takifugu_rubripes LRGLRSCHSG IRWTAGWSLP LGFLLSRNYL SWSKEHPLS- -HDVSGFFRA SCVPGAAALA

XP_003448463_Oreochromis_niloticus LQGRRSCHSG VRWTAGWSLP LGFLLSRNYL SWAKEHPLS- -QDVSTFFSA SCIPGAGAMA

XP_004548831_Maylandia_zebra LQGHRSCHSG VRWTAGWSLP LGFLLSRNYL SWAKEHPLS- -QDVSTFFSA SCIPGAGAMA

XP_005147853_Melopsittacus_undulatus LRGVRSCHNG AKWTSGWNIP LGFLLASNDL SWDKAQPLS- -QAVSEYFNA SCIPGVGVAA

EMP34150_Chelonia_mydas LRGVRSCHNG ARWTSGWNIP LGFLLARNEL LWDEDQPLS- -QVISQYFNA SCIPGVGVTS

XP_002931966_Xenopus_tropicalis LNRTRSCHNG ARWTSGWNIP FGFFLSKNLL HWDEEQPLS- -KVVSAYFNA SCIPGIGIAS

Other

XP_006008475_Latimeria_chalumnae LKGKYSCHTG LGKSAGWVAP IGTLVEMGQI EWGGPEKQPI EQAVANFFAG SCVPGAPRS-

XP_001364584_Monodelphis_domestica LQGKKSCHTG LGRSAGWIMP VGTMYALGLL DWKGPPEP-I EEAVAKLFSG SCVPCAQEK-

NP_001027487_Xenopus_tropicalis LRDKRSCHTG IGKTAGWNVI IGLLLEKQLL KWEGPDTESL EKAVSKFFKA SCVPGAK---

XP_003763732_Sarcophilus_harrisii LKGKKSCHTG LGMSAGWIIP VGKLVELGVL NWNNTNDP-I EKEVATFFSD SCVPCAFWT-

NP_001083070_Xenopus_laevis LKDKRSCHTG IGKTAGWNII IGLLLEKKLL SWGGPDTESL EKAVSRFFKA SCVPGAK---

XP_005796062_Xiphophorus_maculatus LGGKKSCHTG LGKSAGWNIP IGTLISMNVI QWNGIEDKPV EEAVSTFFQA SCAPGATRG-

XP_004542735_Maylandia_zebra LRGKKSCHTG LGKSAGWNIP IGSLVTMNVI EWGGIEDKPL EEAVSTFFHA SCAPGAARG-

XP_003978228_Takifugu_rubripes LAGKKSCHTG LGKSAGWNIP IGTLLSMDLI KWTGIEDSPV EEAVKNFFHS SCVPGANAN-

NP_001116384_Oryzias_latipes LRGKKSCHTG LGKSAGWNIP IGTLVSMDII QWAGVEDKPV EEEVSTFFQA SCVPGATRG-

NP_001079812_Xenopus_laevis LKDKKSCHTG IGKTAGWNII IGLLLERKLL KWAGPDSETW RNAVSKFFKA SCVPGAK---

O subfamily 240

NP_990635_Gallus_gallus -EQKLCRQCK GD------PK TKCARN--AP YSGYSGAFHC LKDGKGDVAF VKHTTVNEN-

XP_003209092_Meleagris_gallopavo -EQKLCRQCK GD------PK TKCARN--AP YSGYSGAFHC LKDGKGDVAF VKHTTVEEN-

XP_005143141_Melopsittacus_undulatus -EQKLCRQCK GD------SK TKCSRT--AP YSGYSGAFHC LKDGKGDVAF VKHTTVQEN-

XP_005489599_Zonotrichia_albicollis -EAKLYRQCK GD------AK TKMSRT--GP YSGYSGAFHC LKDGKGDVAF VKHTTVQEN-

XP_002192726_Taeniopygia_guttata -EAKLYRQCK GD------AK TKMSRT--GP YSGYSGAFQC LKDGKGDVAF VKHTTVQEN-

BAJ23165_Dromaius_novaehollandiae -EKKLCRQCK GD------AK TKCAHG--GP YYGYSGAFRC LKDGNGEVAF VKHTTVEEN-

CAH10347_Struthio_camelus -EKNLCSKCK GD------PK TKCAHT--AL YAGYSGAFLC LKDGNGEVAF VKHTTVEEN-

XP_005050798_Ficedula_albicollis -EPKLYRQCK GD------AK TKMSRT--GP YSGYSGAFHC LKDGKGDVAF VKHTTVQEN-

XP_005303689_Chrysemys_picta_bellii -EPNLCRLCL GT------GA QKCSRT--GP YSGYSGAFQC LKDGAGDVAF VKHTTVLEN-

XP_006037143_Alligator_sinensis -TDKLCRLCI GP------DPCSRS--GP YSGYSGAFQC LKDGAGDVAF VKHTTVSEN-

CAK18228_Lacerta_agilis -EANLCRICA GK------GD EKCSRN--DP YAGYSGAFQC LKDGAGDVAF VKDATVLAL-

CAK18230_Crocodylus_niloticus -TGVLCNLCL ------DSCSHS--SS DSGYSGAFRC LKDGGGDVAF VKHTTVSEN-

CAK18222_Chamaeleo_chamaeleon -EPNLCALCA GK------GQ EKCSRN--DP YSGYSGALEC LKSGAGEVAF VKDKTVLEL-

XP_003218456_Anolis_carolinensis -EPSLCRLCA GV------GS EKCSRN--DP YSGYSGAMDC LKDGVGEVAF VKDATVLAL-

CAK18224_Python_bivittatus -VPNLCRLCA G------NCDRN--DP YAGYSGAFEC LKSGGGDVAF LNEATVLAA-

CAK18223_Boaedon_fuliginosus -VPNLCRACS G------NCDWN--DP FVGYAGAYQC LKSGAGDVAF VNEAVVLAD-

CAK18221_Natrix_natrix -VPNMCRACS G------NCDWN--DP FVGYAGAYQC LKSGAGDVAF VNEAVVLTD-

S subfamily

XP_005600539_Equus_caballus -VPNLCQLCV GK------GT DKCACSNHEP YFGYSGAFKC LADGAGDVAF VKHSTVLEN-

XP_534268_Canis_lupus_familiaris -FPKLCQLCV GK------GT DKCACSNHEP YFGYSGAFKC LMEDAGEVAF VKHSTVLEN-

XP_003265287_Nomascus_leucogenys -FPQLCQLCP E------CGCSTLNQ YFGYSGAFKC LKDGAGDVAF VKHSTIFEN-

XP_005675608_Capra_hircus -FPKLCQLCA GK------GT DKCACSNHEP YFGYAGAFKC LAEGSGDVAF VKHSTVFDN-

NP_001054_Homo_sapiens -FPQLCQLCP G------CGCSTLNQ YFGYSGAFKC LKDGAGDVAF VKHSTIFEN-

NP_803450_Bos_taurus -FPKLCQLCA GK------GT DKCACSNHEP YFGYSGAFKC LMEGAGDVAF VKHSTVFDN-

XP_003476776_Cavia_porcellus -FPQLCQLCP G------CGCSSIQP HFGYAGAFKC LKDGGGDVAF IKHLTIFEA-

XP_004419415_Ceratotherium_simum_simum -FPNLCQLCA GK------GT DKCACSNHEP YFGYSGAFKC LMEDAGEVAF VKHSTVFEN-

XP_004603284_Sorex_araneus -FPKLCQLCA GA------GA DKCACSSHEP YYGYSGALKC LADDAGEVAF VKHLTALAHL

NP_598738_Mus_musculus -FPKLCQLCP G------CGCSSTQP FFGYVGAFKC LKDGGGDVAF VKHTTIFEV-

XP_003789550_Otolemur_garnettii -FPQLCQLCA G------CGCSSLQP YFGYSGAFKC LKDDVGEVAF VKHLTVFEN-

ICA subfamily

XP_005600996_Equus_caballus -FPSLCQLCA GK------GT DKCACSSQEP YFGYSGAFKC LQDGAGDVAF VRHTTVFEN-

XP_005202014_Bos_taurus -FPKLCQLCA GK------GM DKCACSHHEP YFGYSGAFKC LQDGVGDVSF VRHLTVFEN-

XP_005675609_Capra_hircus -FPKLCQLCA GK------GT DKCACSHHEP HFGYSGAFKC LQDGVGDVSF VRHLTVFEN-

XP_004419416_Ceratotherium_simum_simum -FPSLCQLCA GK------GT DKCACSNQEL YFGYSGAFKC LQY-VGDVAF MRHLTVFEN-

XP_004603285_Sorex_araneus -LPRLCQLCA GQ------RT DKCACSSREP YFGYAGAFQC LRDGAGEVSF VRHLTVFEN-

XP_005634506_Canis_lupus_familiaris -FPRLCQLCV GE------GT DKCACSFQEP YFGYSGAFKC LQDGVGDVAF LRHVTVFEN-

NP_082194_Mus_musculus -FPSLCQLCA GK------GT DKCACSSREP YFGSWGALKC LQDGTADVSF VKHLTVFEA-