Table S1: Details of the samples used for microarray analysis

No. / Sample ID / Histological
grade / Classification as per
miRNA signature
1 / Normal1 / NORMAL / NORMAL
2 / Normal2 / NORMAL / NORMAL
3 / Normal3 / NORMAL / NORMAL
4 / Normal5 / NORMAL / NORMAL
5 / Normal6 / NORMAL / NORMAL
6 / Normal7 / NORMAL / NORMAL
7 / Normal8 / NORMAL / NORMAL
8 / CN42 / Anaplastic astrocytoma / Anaplastic astrocytoma
9 / CN43 / Anaplastic astrocytoma / Anaplastic astrocytoma
10 / CN44 / Anaplastic astrocytoma / Anaplastic astrocytoma
11 / CN47 / Anaplastic astrocytoma / Anaplastic astrocytoma
12 / CN48 / Anaplastic astrocytoma / Anaplastic astrocytoma
13 / CN49 / Anaplastic astrocytoma / Anaplastic astrocytoma
14 / CN50 / Anaplastic astrocytoma / Glioblastoma
15 / CN51 / Anaplastic astrocytoma / Glioblastoma
16 / CN52 / Anaplastic astrocytoma / Anaplastic astrocytoma
17 / CN55 / Anaplastic astrocytoma / Anaplastic astrocytoma
18 / CN56 / Anaplastic astrocytoma / Anaplastic astrocytoma
19 / CN57 / Anaplastic astrocytoma / Anaplastic astrocytoma
20 / CN62 / Anaplastic astrocytoma / Anaplastic astrocytoma
21 / CN1 / Primary Glioblastoma / Glioblastoma
22 / CN2 / Primary Glioblastoma / Glioblastoma
23 / CN3 / Primary Glioblastoma / Glioblastoma
24 / CN4 / Primary Glioblastoma / Glioblastoma
25 / CN5 / Primary Glioblastoma / Glioblastoma
26 / CN6 / Primary Glioblastoma / Glioblastoma
27 / CN7 / Primary Glioblastoma / Glioblastoma
28 / CN9 / Primary Glioblastoma / Glioblastoma
29 / CN11 / Primary Glioblastoma / Glioblastoma
30 / CN12 / Primary Glioblastoma / Glioblastoma
31 / CN13 / Primary Glioblastoma / Glioblastoma
32 / CN29 / Primary Glioblastoma / Glioblastoma
33 / CN39 / Primary Glioblastoma / Glioblastoma
34 / CN17 / Secondary glioblastoma / Glioblastoma
35 / CN18 / Secondary glioblastoma / Glioblastoma
36 / CN22 / Secondary glioblastoma / Glioblastoma
37 / CN23 / Secondary glioblastoma / Glioblastoma
38 / CN25 / Secondary glioblastoma / Glioblastoma
39 / CN26 / Secondary glioblastoma / Glioblastoma
40 / CN31 / Secondary glioblastoma / Anaplastic astrocytoma
41 / CN32 / Secondary glioblastoma / Glioblastoma
42 / CN33 / Secondary glioblastoma / Glioblastoma
43 / CN34 / Secondary glioblastoma / Glioblastoma
44 / CN35 / Secondary glioblastoma / Glioblastoma
45 / CN36 / Secondary glioblastoma / Anaplastic astrocytoma
46 / CN37 / Secondary glioblastoma / Glioblastoma

Table S2: Differentially regulated miRNAs in malignant astrocytoma

I- miRNAs up regulated in malignant astrocytoma compared to normals
No / Annotation / Median of normalsa (log2) / Median of astrocytomaa (log2) / Difference
(log2) / Fold change
1 / hsa-miR-21 / 0.34 / 4.17 / 3.83 / 14.20
2 / hsa-miR-10b / -0.18 / 2.89 / 3.07 / 8.38
3 / hsa-miR-92b / 0.00 / 2.31 / 2.31 / 4.97
4 / hsa-miR-25 / -0.01 / 2.04 / 2.05 / 4.13
5 / hsa-miR-195 / 0.03 / 2.05 / 2.02 / 4.06
6 / hsa-miR-193a-3p / -0.18 / 1.81 / 1.99 / 3.96
7 / hsa-miR-106b / 0.14 / 2.11 / 1.97 / 3.92
8 / hsa-miR-130a / -0.01 / 1.90 / 1.91 / 3.76
9 / hsa-miR-10a / 0.02 / 1.85 / 1.83 / 3.55
10 / hsa-miR-23a / 0.07 / 1.83 / 1.76 / 3.39
11 / hsa-miR-19b / -0.01 / 1.75 / 1.76 / 3.39
12 / hsa-miR-93 / 0.13 / 1.89 / 1.76 / 3.38
13 / hsa-miR-105 / 0.11 / 1.76 / 1.65 / 3.13
14 / hsa-miR-19a / 0.00 / 1.62 / 1.63 / 3.08
15 / hsa-miR-497 / 0.10 / 1.71 / 1.61 / 3.06
16 / hsa-miR-15b / -0.05 / 1.51 / 1.56 / 2.96
17 / hsa-miR-142-3p / 0.20 / 1.75 / 1.55 / 2.93
18 / hsa-miR-20b / 0.08 / 1.60 / 1.52 / 2.87
19 / hsa-miR-142-5p / 0.06 / 1.56 / 1.50 / 2.84
20 / hsa-miR-182 / 0.10 / 1.59 / 1.49 / 2.80
21 / hsa-miR-542-5p / -0.04 / 1.35 / 1.39 / 2.62
22 / hsa-miR-199a-3p/hsa-miR-199b-3p / 0.19 / 1.52 / 1.33 / 2.51
23 / hsa-miR-16 / 0.08 / 1.34 / 1.27 / 2.41
24 / hsa-miR-106a / 0.21 / 1.48 / 1.26 / 2.40
25 / hsa-miR-130b / -0.13 / 1.09 / 1.23 / 2.34
26 / hsa-miR-15a / 0.38 / 1.59 / 1.21 / 2.31
27 / hsa-miR-155 / 0.19 / 1.38 / 1.19 / 2.28
28 / hsa-miR-505 / -0.12 / 1.07 / 1.18 / 2.27
29 / hsa-miR-32 / 0.00 / 1.16 / 1.16 / 2.24
30 / hsa-miR-17 / 0.24 / 1.39 / 1.15 / 2.22
31 / hsa-miR-144 / 0.26 / 1.41 / 1.15 / 2.21
32 / hsa-miR-374b / 0.15 / 1.29 / 1.14 / 2.21
33 / hsa-miR-99a / 0.12 / 1.26 / 1.14 / 2.21
34 / hsa-miR-146a / 0.04 / 1.18 / 1.13 / 2.20
35 / hsa-miR-152 / 0.00 / 1.12 / 1.13 / 2.18
36 / hsa-miR-320a / -0.02 / 1.10 / 1.12 / 2.18
37 / hsa-miR-374a / 0.40 / 1.52 / 1.12 / 2.17
38 / hsa-miR-450a / 0.02 / 1.12 / 1.09 / 2.14
39 / hsa-miR-362-3p / 0.00 / 1.07 / 1.07 / 2.10
40 / hsa-miR-96 / -0.01 / 1.05 / 1.06 / 2.08
41 / hsa-miR-9 / 0.48 / 1.50 / 1.02 / 2.03
42 / hsa-miR-424 / 0.11 / 1.13 / 1.02 / 2.03
43 / hsa-miR-550 / -0.03 / 0.97 / 1.01 / 2.01
44 / hsa-miR-532-5p / -0.09 / 0.91 / 1.00 / 2.00
45 / hsa-miR-215 / -0.02 / 0.98 / 1.00 / 2.00
46 / hsa-miR-92a / 0.08 / 1.06 / 0.98 / 1.97
47 / hsa-miR-106b* / -0.06 / 0.92 / 0.98 / 1.97
48 / hsa-miR-660 / -0.01 / 0.93 / 0.94 / 1.92
49 / hsa-miR-20b* / -0.05 / 0.85 / 0.91 / 1.88
50 / hsa-miR-17* / -0.15 / 0.67 / 0.82 / 1.76
51 / hsa-miR-199b-5p / 0.12 / 0.89 / 0.78 / 1.71
52 / hsa-miR-135b / 0.32 / 1.02 / 0.71 / 1.63
53 / hsa-miR-181a-2* / -0.01 / 0.66 / 0.67 / 1.59
54 / hsa-miRPlus-A1083 / -0.02 / 0.62 / 0.64 / 1.56
55 / hsa-miR-122* / 0.04 / 0.50 / 0.46 / 1.38
II-miRNAs down regulated in malignant astrocytoma compared to normals
No. / Annotation / Median of normals / Median of astrocytoma / Difference / Fold change
1 / hsa-miR-124 / 0.01 / -3.85 / -3.86 / 14.48
2 / hsa-miR-129-3p / 0.00 / -3.36 / -3.36 / 10.29
3 / hsa-miR-138 / 0.09 / -2.47 / -2.56 / 5.91
4 / hsa-miR-638 / 0.48 / -1.64 / -2.12 / 4.35
5 / hsa-miRPlus-A1056 / 0.33 / -1.77 / -2.10 / 4.29
6 / hsa-miR-637 / 0.31 / -1.77 / -2.08 / 4.22
7 / hsa-miR-128 / 0.04 / -2.02 / -2.07 / 4.18
8 / hsa-miR-338-3p / 0.10 / -1.88 / -1.98 / 3.95
9 / hsa-miR-129* / -0.01 / -1.98 / -1.97 / 3.92
10 / hsa-miR-323-3p / 0.14 / -1.79 / -1.93 / 3.80
11 / hsa-miRPlus-D1036 / 0.02 / -1.84 / -1.86 / 3.64
12 / hsa-miR-219-2-3p / -0.11 / -1.97 / -1.86 / 3.63
13 / hsa-miR-132 / 0.27 / -1.45 / -1.72 / 3.29
14 / hsa-miR-1908 / 0.13 / -1.57 / -1.70 / 3.26
15 / hsa-miR-129-5p / -0.06 / -1.76 / -1.70 / 3.25
16 / hsa-miR-219-5p / -0.43 / -2.12 / -1.69 / 3.23
17 / hsa-miR-483-5p / 0.01 / -1.65 / -1.66 / 3.15
18 / hsa-miR-329 / 0.03 / -1.61 / -1.64 / 3.12
19 / hsa-miRPlus-A1027 / 0.17 / -1.39 / -1.56 / 2.95
20 / hsa-miR-198 / 0.03 / -1.49 / -1.52 / 2.88
21 / hsa-miR-665 / -0.02 / -1.52 / -1.50 / 2.83
22 / hsa-miR-628-3p / 0.13 / -1.30 / -1.43 / 2.69
23 / hsa-miR-187* / -0.10 / -1.38 / -1.29 / 2.44
24 / hsa-miR-338-5p / -0.11 / -1.24 / -1.13 / 2.18
25 / hsa-miR-765 / -0.05 / -1.16 / -1.11 / 2.16
26 / hsa-miR-432 / 0.04 / -0.98 / -1.02 / 2.03
27 / hsa-miR-498 / 0.06 / -0.80 / -0.85 / 1.81
28 / hsa-miR-485-3p / 0.03 / -0.81 / -0.83 / 1.78
29 / hsa-miR-584 / -0.08 / -0.56 / -0.47 / 1.39

a - median log2 ratio of 7 normal samples and 39 astrocytoma (13 anaplastic astrocytoma and 26 glioblastoma) samples are shown

Table S3: Differentially regulated miRNAs between glioblastoma and anaplastic astrocytoma.

I- miRNAs up regulated in glioblastoma compared to anaplastic astrocytoma
No. / Annotation / Median of anaplastic astrocytomaa(log2) / Median of glioblastomaa(log2) / Difference
(log2) / Fold change
1 / hsa-miR-532-5p / 0.40 / 0.99 / 0.60 / 1.51
2 / hsa-miR-24-1* / 0.09 / 0.75 / 0.66 / 1.58
3 / hsa-miR-144 / 0.68 / 1.75 / 1.07 / 2.10
4 / hsa-miRPlus-B1114 / -0.24 / 0.35 / 0.59 / 1.51
5 / hsa-miR-19b / 0.79 / 2.02 / 1.23 / 2.35
6 / hsa-miR-602 / -0.32 / 0.26 / 0.59 / 1.50
7 / hsa-miR-26a / 0.04 / 0.52 / 0.48 / 1.39
8 / hsa-miR-374a / 0.79 / 1.75 / 0.96 / 1.95
9 / hsa-miR-23a / 1.19 / 2.07 / 0.88 / 1.84
10 / hsa-miR-214 / 0.13 / 0.57 / 0.44 / 1.36
11 / hsa-miR-337-3p / 0.18 / 0.97 / 0.79 / 1.73
12 / hsa-miR-339-5p / 0.24 / 1.19 / 0.95 / 1.93
13 / hsa-miR-363* / 0.16 / 1.11 / 0.96 / 1.94
14 / hsa-miR-193b / 0.06 / 1.09 / 1.02 / 2.03
15 / hsa-miR-145 / -0.33 / 0.62 / 0.95 / 1.93
16 / hsa-miR-106b / 1.09 / 2.32 / 1.23 / 2.34
17 / hsa-miR-23b / -0.60 / 0.30 / 0.89 / 1.86
18 / hsa-miR-886-3p / -0.71 / 1.22 / 1.93 / 3.82
19 / hsa-miRPlus-A1087 / -0.21 / 1.21 / 1.42 / 2.68
20 / hsa-miR-130a / 0.79 / 2.01 / 1.22 / 2.34
21 / hsa-miR-126* / 0.15 / 0.92 / 0.77 / 1.70
22 / hsa-miR-377 / -0.99 / 0.10 / 1.08 / 2.12
23 / hsa-miR-15a / 0.68 / 1.75 / 1.07 / 2.09
24 / hsa-miR-146a / 0.34 / 1.38 / 1.04 / 2.06
25 / hsa-miR-409-3p / -1.27 / -0.10 / 1.17 / 2.25
26 / hsa-miR-668 / 0.26 / 1.04 / 0.78 / 1.71
27 / hsa-miR-411 / -1.15 / -0.19 / 0.96 / 1.95
28 / hsa-miR-551b / -0.57 / 0.10 / 0.67 / 1.59
29 / hsa-miR-487a / -1.14 / -0.16 / 0.97 / 1.96
30 / hsa-miR-886-5p / -0.60 / 1.32 / 1.91 / 3.77
31 / hsa-miR-337-5p / -0.38 / 0.86 / 1.24 / 2.37
32 / hsa-miR-376b / -0.12 / 0.75 / 0.87 / 1.83
33 / hsa-miR-923 / -0.67 / 0.72 / 1.39 / 2.62
34 / hsa-miR-34a / 0.25 / 1.69 / 1.45 / 2.73
35 / hsa-miR-451 / 0.22 / 1.82 / 1.61 / 3.04
36 / hsa-miR-191 / -0.09 / 0.70 / 0.80 / 1.74
37 / hsa-miR-199a-3p/hsa-miR-199b-3p / 0.47 / 1.85 / 1.38 / 2.60
38 / hsa-miR-146b-5p / 0.20 / 1.49 / 1.30 / 2.46
39 / hsa-miR-15b / 0.82 / 1.90 / 1.08 / 2.12
40 / hsa-miR-376c / -0.83 / 0.98 / 1.80 / 3.49
41 / hsa-miR-21 / 2.47 / 4.81 / 2.34 / 5.05
42 / hsa-miR-142-5p / 0.22 / 1.71 / 1.49 / 2.81
43 / hsa-miR-24 / -0.47 / 0.67 / 1.14 / 2.21
44 / hsa-miR-193a-3p / 0.34 / 1.90 / 1.56 / 2.95
45 / hsa-miR-22 / -1.03 / 0.25 / 1.28 / 2.42
46 / hsa-miR-378 / 0.01 / 0.86 / 0.85 / 1.81
47 / hsa-miR-552 / -0.12 / 1.11 / 1.22 / 2.33
48 / hsa-miR-215 / 0.47 / 1.13 / 0.66 / 1.58
49 / hsa-miR-155 / 0.46 / 1.81 / 1.35 / 2.54
50 / hsa-miR-513a-5p / 0.13 / 1.18 / 1.05 / 2.08
51 / hsa-miR-16 / 0.14 / 1.64 / 1.50 / 2.83
52 / hsa-miR-143 / -0.31 / 1.17 / 1.48 / 2.79
53 / hsa-miR-335 / 0.11 / 1.57 / 1.46 / 2.76
54 / hsa-miR-509-3-5p / -0.07 / 1.14 / 1.21 / 2.31
55 / hsa-miR-381 / -0.71 / 0.49 / 1.19 / 2.29
56 / hsa-miR-126 / -0.27 / 1.31 / 1.58 / 3.00
II- miRNAs down regulated in glioblastoma compared to anaplastic astrocytoma
No. / Annotation / Median of anaplastic astrocytoma (log2) / Median of glioblastoma (log2) / Difference (log2) / Fold change
1 / hsa-miR-129-5p / -0.86 / -1.87 / -1.01 / 2.01
2 / hsa-miR-219-2-3p / -0.28 / -2.64 / -2.36 / 5.13
3 / hsa-miR-638 / -0.50 / -1.68 / -1.18 / 2.26
4 / hsa-miR-125b-1* / -0.31 / -0.77 / -0.47 / 1.38
5 / hsa-miR-625* / -0.01 / -0.50 / -0.49 / 1.40
6 / hsa-miR-128 / -0.78 / -2.39 / -1.61 / 3.05
7 / hsa-miR-637 / -0.65 / -1.88 / -1.23 / 2.35
8 / hsa-miR-933 / 0.22 / -0.65 / -0.87 / 1.83
9 / hsa-miRPlus-A1056 / -0.73 / -1.87 / -1.14 / 2.21
10 / hsa-miR-659 / -0.02 / -0.84 / -0.82 / 1.76
11 / hsa-miR-338-3p / -1.37 / -2.38 / -1.00 / 2.00

a - median log2 ratio of 13 anaplastic astrocytoma samples and 26 glioblastoma samples are shown.

Table S4: Differentially regulated miRNAs between anaplastic astrocytoma and secondary glioblastoma.

I- miRNAs up regulated in secondary glioblastomacompared to anaplastic astrocytoma
No. / Annotation / Median of anaplastic astrocytomaa(log2) / Median of secondary glioblastoma(log2) / Difference (log2) / Fold change
1 / hsa-miR-10b / 1.83 / 4.82 / 2.99 / 7.92
2 / hsa-miR-21 / 2.47 / 5.11 / 2.65 / 6.26
3 / hsa-miR-376c / -0.83 / 1.24 / 2.07 / 4.19
4 / hsa-miR-451 / 0.22 / 2.25 / 2.03 / 4.08
5 / hsa-miR-142-5p / 0.22 / 2.08 / 1.86 / 3.63
6 / hsa-miR-19b / 0.79 / 2.57 / 1.78 / 3.44
7 / hsa-miR-130a / 0.79 / 2.56 / 1.77 / 3.41
8 / hsa-miR-9* / -0.10 / 1.55 / 1.65 / 3.14
9 / hsa-miR-16 / 0.14 / 1.77 / 1.63 / 3.09
10 / hsa-miR-105 / 0.94 / 2.52 / 1.58 / 3.00
11 / hsa-miR-19a / 0.61 / 2.19 / 1.58 / 2.98
12 / hsa-miR-106b / 1.09 / 2.64 / 1.55 / 2.93
13 / hsa-miR-143 / -0.31 / 1.18 / 1.49 / 2.80
14 / hsa-miR-193a-3p / 0.34 / 1.79 / 1.45 / 2.73
15 / hsa-miR-409-3p / -1.27 / 0.11 / 1.38 / 2.61
16 / hsa-miR-411 / -1.15 / 0.23 / 1.38 / 2.61
17 / hsa-miR-923 / -0.67 / 0.69 / 1.36 / 2.58
18 / hsa-miR-199a-3p/hsa-miR-199b-3p / 0.47 / 1.81 / 1.34 / 2.54
19 / hsa-miR-126 / -0.27 / 1.06 / 1.33 / 2.52
20 / hsa-miR-337-5p / -0.38 / 0.92 / 1.30 / 2.46
21 / hsa-miR-10a / 1.16 / 2.45 / 1.29 / 2.44
22 / hsa-miR-34a / 0.25 / 1.51 / 1.26 / 2.40
23 / hsa-miR-552 / -0.12 / 1.10 / 1.22 / 2.33
24 / hsa-miR-509-3-5p / -0.07 / 1.14 / 1.22 / 2.32
25 / hsa-miR-335 / 0.11 / 1.31 / 1.21 / 2.31
26 / hsa-miR-374a / 0.79 / 1.98 / 1.20 / 2.29
27 / hsa-miR-513a-5p / 0.13 / 1.31 / 1.18 / 2.27
28 / hsa-miR-15a / 0.68 / 1.86 / 1.18 / 2.26
29 / hsa-miR-22 / -1.03 / 0.15 / 1.17 / 2.26
30 / hsa-miR-381 / -0.71 / 0.46 / 1.17 / 2.25
31 / hsa-miR-144 / 0.68 / 1.83 / 1.15 / 2.22
32 / hsa-miR-15b / 0.82 / 1.96 / 1.15 / 2.21
33 / hsa-miR-24 / -0.47 / 0.67 / 1.14 / 2.21
34 / hsa-miR-130b / 0.57 / 1.69 / 1.12 / 2.18
35 / hsa-miR-369-3p / -0.66 / 0.45 / 1.11 / 2.16
36 / hsa-miR-487a / -1.14 / -0.04 / 1.10 / 2.14
37 / hsa-miR-377 / -0.99 / 0.10 / 1.08 / 2.12
38 / hsa-miR-376b / -0.12 / 0.89 / 1.01 / 2.01
39 / hsa-miR-146b-5p / 0.20 / 1.19 / 0.99 / 1.99
40 / hsa-miR-23b / -0.60 / 0.39 / 0.99 / 1.98
41 / hsa-miR-155 / 0.46 / 1.44 / 0.98 / 1.97
42 / hsa-miR-23a / 1.19 / 2.10 / 0.91 / 1.88
43 / hsa-miR-378 / 0.01 / 0.85 / 0.84 / 1.79
44 / hsa-miR-337-3p / 0.18 / 0.97 / 0.79 / 1.73
45 / hsa-miR-191 / -0.09 / 0.69 / 0.79 / 1.72
46 / hsa-miR-146a / 0.34 / 1.12 / 0.79 / 1.72
47 / hsa-miR-363* / 0.16 / 0.92 / 0.77 / 1.70
48 / hsa-miR-136* / -0.73 / -0.02 / 0.71 / 1.64
49 / hsa-miR-551b / -0.57 / 0.11 / 0.67 / 1.59
50 / hsa-miR-215 / 0.47 / 1.13 / 0.66 / 1.58
51 / hsa-miR-199b-5p / 0.43 / 1.05 / 0.62 / 1.54
52 / hsa-miR-376a* / -0.91 / -0.29 / 0.62 / 1.54
53 / hsa-miR-126* / 0.15 / 0.74 / 0.59 / 1.51
54 / hsa-miR-516a-5p / -0.50 / 0.10 / 0.59 / 1.51
55 / hsa-miR-668 / 0.26 / 0.82 / 0.56 / 1.47
56 / hsa-miR-602 / -0.32 / 0.22 / 0.54 / 1.46
57 / hsa-miR-26a / 0.04 / 0.53 / 0.48 / 1.40
58 / hsa-miRPlus-B1114 / -0.24 / 0.22 / 0.45 / 1.37
59 / hsa-miR-938 / 0.55 / 1.01 / 0.45 / 1.37
60 / hsa-miR-214 / 0.13 / 0.56 / 0.43 / 1.35
II- miRNAs down regulated in secondary glioblastoma compared to anaplastic astrocytoma
No. / Annotation / Median of
anaplastic astrocytoma (log2) / Median of
secondary glioblastoma (log2) / Difference
(log2) / Fold change
1 / hsa-miR-219-2-3p / -0.28 / -2.56 / -2.28 / 4.86
2 / hsa-miR-128 / -0.78 / -2.41 / -1.63 / 3.10
3 / hsa-miR-638 / -0.50 / -1.68 / -1.18 / 2.26
4 / hsa-miR-129-5p / -0.86 / -1.96 / -1.10 / 2.14
5 / hsa-miR-933 / 0.22 / -0.76 / -0.98 / 1.98
6 / hsa-miR-338-3p / -1.37 / -2.32 / -0.94 / 1.92
7 / hsa-miR-125b-1* / -0.31 / -0.82 / -0.51 / 1.42
8 / hsa-miR-625* / -0.01 / -0.52 / -0.51 / 1.42

a - median log2 ratio of 13 anaplastic astrocytoma samples and 13 secondary glioblastoma samples are shown.

Table S5: Differentially regulated miRNAs between anaplastic astrocytoma and primary glioblastoma.

I- miRNAs up regulated in primary glioblastoma compared to anaplastic astrocytoma
No. / Annotation / Median of
anaplastic astrocytomaa (log2) / Median of
primary glioblastomaa (log2) / Difference
(log2) / Fold change
1 / hsa-miR-886-3p / -0.71 / 2.84 / 3.55 / 11.68
2 / hsa-miR-886-5p / -0.60 / 2.83 / 3.43 / 10.79
3 / hsa-miRPlus-A1087 / -0.21 / 2.58 / 2.79 / 6.92
4 / hsa-miR-21 / 2.47 / 4.41 / 1.94 / 3.83
5 / hsa-miR-146b-5p / 0.20 / 2.01 / 1.82 / 3.52
6 / hsa-miR-222 / -2.00 / -0.21 / 1.79 / 3.46
7 / hsa-miR-221 / -1.56 / 0.15 / 1.71 / 3.27
8 / hsa-miR-126 / -0.27 / 1.43 / 1.70 / 3.24
9 / hsa-miR-34a / 0.25 / 1.83 / 1.58 / 3.00
10 / hsa-miR-193a-3p / 0.34 / 1.92 / 1.58 / 2.99
11 / hsa-miR-335 / 0.11 / 1.67 / 1.56 / 2.95
12 / hsa-miR-155 / 0.46 / 1.96 / 1.50 / 2.82
13 / hsa-miR-143 / -0.31 / 1.17 / 1.47 / 2.78
14 / hsa-miR-142-5p / 0.22 / 1.67 / 1.45 / 2.74
15 / hsa-miR-923 / -0.67 / 0.74 / 1.41 / 2.66
16 / hsa-miR-199a-3p/hsa-miR-199b-3p / 0.47 / 1.86 / 1.39 / 2.62
17 / hsa-miR-22 / -1.03 / 0.34 / 1.37 / 2.58
18 / hsa-miR-376c / -0.83 / 0.50 / 1.32 / 2.50
19 / hsa-miR-16 / 0.14 / 1.41 / 1.27 / 2.41
20 / hsa-miR-204 / -0.38 / 0.87 / 1.26 / 2.39
21 / hsa-miR-552 / -0.12 / 1.11 / 1.23 / 2.34
22 / hsa-miR-451 / 0.22 / 1.44 / 1.22 / 2.33
23 / hsa-miR-381 / -0.71 / 0.51 / 1.22 / 2.33
24 / hsa-miR-337-5p / -0.38 / 0.81 / 1.19 / 2.29
25 / hsa-miR-509-3-5p / -0.07 / 1.11 / 1.19 / 2.27
26 / hsa-miR-193b / 0.06 / 1.23 / 1.17 / 2.24
27 / hsa-miR-130a / 0.79 / 1.95 / 1.17 / 2.24
28 / hsa-miR-142-3p / 0.88 / 2.03 / 1.15 / 2.22
29 / hsa-miR-24 / -0.47 / 0.67 / 1.14 / 2.21
30 / hsa-miR-146a / 0.34 / 1.47 / 1.14 / 2.20
31 / hsa-miR-363* / 0.16 / 1.26 / 1.11 / 2.16
32 / hsa-miR-409-3p / -1.27 / -0.19 / 1.08 / 2.12
33 / hsa-miR-145 / -0.33 / 0.73 / 1.06 / 2.08
34 / hsa-miR-377 / -0.99 / 0.07 / 1.05 / 2.07
35 / hsa-miR-513a-5p / 0.13 / 1.16 / 1.04 / 2.06
36 / hsa-miR-339-5p / 0.24 / 1.28 / 1.04 / 2.05
37 / hsa-miR-495 / -1.54 / -0.51 / 1.03 / 2.05
38 / hsa-miR-15a / 0.68 / 1.69 / 1.01 / 2.01
39 / hsa-miR-15b / 0.82 / 1.79 / 0.98 / 1.97
40 / hsa-miR-668 / 0.26 / 1.18 / 0.92 / 1.89
41 / hsa-miR-23b / -0.60 / 0.30 / 0.89 / 1.86
42 / hsa-miR-126* / 0.15 / 1.03 / 0.88 / 1.85
43 / hsa-miR-378 / 0.01 / 0.89 / 0.88 / 1.84
44 / hsa-miR-191 / -0.09 / 0.78 / 0.87 / 1.83
45 / hsa-miR-152 / 0.50 / 1.35 / 0.86 / 1.81
46 / hsa-miR-23a / 1.19 / 2.05 / 0.86 / 1.81
47 / hsa-miR-337-3p / 0.18 / 0.98 / 0.81 / 1.75
48 / hsa-miR-411 / -1.15 / -0.35 / 0.81 / 1.75
49 / hsa-miR-363 / 0.49 / 1.28 / 0.79 / 1.73
50 / hsa-miR-376b / -0.12 / 0.65 / 0.77 / 1.71
51 / hsa-miR-365 / -0.23 / 0.51 / 0.74 / 1.67
52 / hsa-miR-24-1* / 0.09 / 0.77 / 0.68 / 1.60
53 / hsa-miR-551b / -0.57 / 0.10 / 0.67 / 1.59
54 / hsa-miR-215 / 0.47 / 1.13 / 0.66 / 1.58
55 / hsa-miRPlus-B1114 / -0.24 / 0.42 / 0.66 / 1.58
56 / hsa-miR-214 / 0.13 / 0.74 / 0.61 / 1.52
57 / hsa-miR-532-5p / 0.40 / 0.99 / 0.60 / 1.51
58 / hsa-miR-379 / -1.12 / -0.53 / 0.59 / 1.51
59 / hsa-miR-516a-5p / -0.50 / 0.10 / 0.59 / 1.51
60 / hsa-miR-602 / -0.32 / 0.26 / 0.59 / 1.50
61 / hsa-miR-199a-5p / 0.58 / 1.13 / 0.55 / 1.46
62 / hsa-miR-425 / -0.22 / 0.30 / 0.52 / 1.43
63 / hsa-miR-26a / 0.04 / 0.52 / 0.48 / 1.39
64 / hsa-miR-874 / -0.53 / -0.19 / 0.35 / 1.27
II- miRNAs down regulated in primary glioblastoma compared to anaplastic astrocytoma
Sr. No. / Annotation / Median of anaplastic astrocytomaa(log2) / Median of
primary glioblastomaa (log2) / Difference
(log2) / Fold change
1 / hsa-miR-219-2-3p / -0.28 / -2.72 / -2.44 / 5.42
2 / hsa-miR-128 / -0.78 / -2.37 / -1.59 / 3.01
3 / hsa-miR-637 / -0.65 / -1.98 / -1.33 / 2.51
4 / hsa-miR-483-5p / -0.69 / -1.91 / -1.21 / 2.32
5 / hsa-miR-638 / -0.50 / -1.70 / -1.20 / 2.29
6 / hsa-miRPlus-A1056 / -0.73 / -1.88 / -1.16 / 2.23
7 / hsa-miR-129-5p / -0.86 / -1.87 / -1.01 / 2.01
8 / hsa-miR-659 / -0.02 / -0.86 / -0.84 / 1.79
9 / hsa-miRPlus-A1073 / 0.42 / -0.20 / -0.62 / 1.54
10 / hsa-miR-125b-1* / -0.31 / -0.76 / -0.45 / 1.37
11 / hsa-miR-625* / -0.01 / -0.46 / -0.45 / 1.36
12 / hsa-miR-625 / -0.26 / -0.64 / -0.38 / 1.30

a - median log2 ratio of 13 anaplastic astrocytoma samples and 13 primary glioblastoma samples are shown.

Table S6: miRNAs differentially regulated between progressive pathway and de novo pathway

No. / Annotation / Median of progressive typeA
(log2) / Median of de novo typeA (log2) / Difference
(log2) / Fold
change
1 / hsa-miR-886-3p / -0.45 / 2.84 / 3.29 / 9.77
2 / hsa-miR-886-5p / -0.28 / 2.83 / 3.11 / 8.66
3 / hsa-miRPlus-A1087 / 0.04 / 2.58 / 2.54 / 5.82
4 / hsa-miR-222 / -1.96 / -0.21 / 1.75 / 3.36
5 / hsa-miR-221 / -1.47 / 0.15 / 1.62 / 3.07
6 / hsa-miR-146b-5p / 0.83 / 2.01 / 1.18 / 2.27
7 / hsa-miR-126 / 0.29 / 1.43 / 1.13 / 2.19
8 / hsa-miR-34a / 0.81 / 1.83 / 1.01 / 2.02
9 / hsa-miR-204 / -0.10 / 0.87 / 0.97 / 1.96
10 / hsa-miR-335 / 0.75 / 1.67 / 0.91 / 1.88
11 / hsa-miR-152 / 0.54 / 1.35 / 0.82 / 1.76
12 / hsa-miR-199a-3p/hsa-miR-199b-3p / 1.05 / 1.86 / 0.81 / 1.76
13 / hsa-miR-193b / 0.45 / 1.23 / 0.78 / 1.72
14 / hsa-miR-381 / -0.22 / 0.51 / 0.74 / 1.66
15 / hsa-miR-143 / 0.52 / 1.17 / 0.65 / 1.57
16 / hsa-miR-509-3-5p / 0.56 / 1.11 / 0.55 / 1.46
17 / hsa-miR-339-5p / 0.76 / 1.28 / 0.53 / 1.44
18 / hsa-miR-193a-3p / 1.42 / 1.92 / 0.49 / 1.41
19 / hsa-miR-126* / 0.57 / 1.03 / 0.47 / 1.38
20 / hsa-miR-145 / 0.34 / 0.73 / 0.39 / 1.31
21 / hsa-miR-378 / 0.52 / 0.89 / 0.37 / 1.29

a median log2 ratio of 26 progressive astrocytoma (13 anaplastic astrocytoma and 13 secondary glioblastoma) samples and 13 primary glioblastoma samples are shown.

Table S7: Differentially regulated miRNAs between secondary glioblastoma and primary glioblastoma.

No. / Annotation / Median of secondary glioblastomaa(log2) / Median of primary glioblastomaa(log2) / Difference
(log2) / Fold change
I- miRNAs up regulated in secondary glioblastoma compared to primary glioblastoma
1 / hsa-miR-19a / 2.19 / 1.58 / 0.61 / 1.53
2 / hsa-miR-19b / 2.57 / 1.57 / 1.00 / 2.00
II- miRNAs down regulated in secondary glioblastoma compared to primary glioblastoma
1 / hsa-miR-886-3p / 0.21 / 2.84 / -2.63 / 6.19
2 / hsa-miRPlus-A1087 / 0.57 / 2.58 / -2.00 / 4.01
3 / hsa-miR-886-5p / 0.66 / 2.83 / -2.17 / 4.52
4 / hsa-miR-222 / -1.92 / -0.21 / -1.71 / 3.27
5 / hsa-miR-221 / -0.94 / 0.15 / -1.09 / 2.13

amedian log2 ratio of 13 secondary glioblastoma samples and 13 primary glioblastoma samples are shown.

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