7
Supplemental Table 1. Primers designed to amplify exons of KAS I on Gm05 (LG-A1).
Exon no. / Primer ID / Primer sequence 5’-> 3’ / Size(bp)
1 / S78_2955K-F1 / GCC ATT GGT TAC ATA AAC GC / 676
S78_2955K-R1 / AAA TAA ACA AAT TTC AAT TCA ACC CG
2 / S78_2955K-F2 / TGG ATT GAA GCT TTC ACC C / 644
S78_2955K-R2 / TCG AAT CTT CTA ATG CAA ACC TTT AT
3 / S78_2955K-F3 / AGT AAT TCA CAT AAT AAA GGT TTG CAT / 551
S78_2955K-R3 / ATT CCT GTG AGA CAA AGC CC
3,4 / S78_2955K-F4 / CCC AAT TAT TCC ATT TCC ACT / 607
S78_2955K-R4 / CTG GTT ACT TGG TAA TAC TCT CTA TTT
5,6 / S78_2955K-F5 / TAA TTG GCT TGG CTA ACC TCT A / 532
S78_2955K-R5 / TAC CAA CAT TCA CTT CGT GC
7,8 / S78_2955K-F6 / GCC ATA GCA ACA ATC AAA GC / 652
S78_2955K-R6 / ATT CGT AAT GTA ACT TTA GCA AAG G
Supplemental Table 2. Primers designed to amplify exons of KAS I on Gm18 (LG-G).
Exon no. / Primer ID / Primer sequence 5’-> 3’ / Size(bp)
Long-range / Gm18-KASI-LR-F / GCG GCA GAC GTG TTA CGT GTA TGT / 3000
Gm18-KASI-LR-R / TCT ACC TGG GAG AGG GGT TTC AGA
1 / S4_3180K-F1 / CCG TAA ATA GGA GGA CGT AAA C / 537
S4_3180K-R1 / GTC CTT ACA ACA AAT CAA TCG C
2 / S4_3180K-F2 / AAT GAG GAG GAT TGG TAC G / 401
S4_3180K-R2 / TTT CTG TGA GAC AAA GCC C
2,3 / S4_3180K-F3 / GAT ATC ATG GTG GTT GGT GGA A / 504
S4_3180K-R3 / TGG TAG TTG GTA GTA CTC TCT ATT
4,5 / S4_3180K-F4 / GTA CTA CCA ACT ACC AAG GC / 572
S4_3180K-R4 / ATC TTT ATT TGC ACA AAG TGA GAG
Supplemental Table 3. Primers designed to amplify exons of accB-1 on Gm18 (LG-G).
Exon no. / Primer ID / Primer sequence 5’-> 3’ / Size (bp)1,2 / AccB-1-SEQ F1 / AGT GTA ATA AGA TAG TTC TGG ATG TAG / 530
AccB-1-SEQ R1 / CTC GTG CAT AAC AGC GT
2 / AccB-1-SEQ F2 / AAT GTT CCA AAT TAC ACA CCA C / 619
AccB-1-SEQ R2 / TTC ATA GGA GGA GAA GTA GCA
3 / AccB-1-SEQ F3 / GAA GTT CAT CCA AAC ATC ACT AGA / 487
AccB-1-SEQ R3 / CAC AGA AGG TTG AGG AGC
4,5 / AccB-1-SEQ F4 / GCA TGA CGT TGA AGT AAC AAT C / 518
AccB-1-SEQ R4 / CAA ATA AGT GCC CAC ATA AAG GTA TAA
6 / AccB-1-SEQ F5 / GTG AAA TCA ATT GGA CTC ATT ACA T / 533
AccB-1-SEQ R5 / CAC TTC CTC TAC TAT TCA AAT ACT CTG
7 / AccB-1-SEQ F6 / GTA TAT TCT AGG GCC TCC TGA TTA / 514
AccB-1-SEQ R6 / AAT CAT GTC CCT CAG CTT
Supplemental Table 4. Sequencing primers targeting the SNP markers used to evaluate for polymorphisms between N00-3350 and Boggs. NS in the SNP column designates that no sequence was obtained from this primer pair due to multiple amplicons or low quality sequence.
STS / SEQEUNCE (5’ -> 3’) / PCRsize
(bp) / SNP
cqOle-002 on Gm17 (LG-D2)
SNP16289-F / CAT AGT CTA ATC ATT CTT CAA CTC CT / 401 / 2
SNP16289-R / TTC GTT GCA TAA GAA GCC C
SNP21991-F / AAA CCC GAA CAC GAA TTG / 517 / 0
SNP21991-R / TTT CCA TGT TTG GCA GAA ATT ATA C
SNP47685-F / AAG AAC AAA GAT GCA ATC CTC AA / 798 / NS
SNP47685-R / TAA GAT GGA TAA GTG GTT CCA GG
SNP60377-F / TAA CAA GGA CGT TTA AGA CCC AA / 473 / 0
SNP60377-R / TAG GGC TGA AAT AAA TGT AGG CA
SNP60523-F / CCA AAA TGT TAA TGC AAT CTG GT / 330 / NS
SNP60523-R / ACG GTT ACA AAC AAT GTG CTT CT
cqOle-005 on Gm19 (LG-L)
SNP11793-F / GAC CTT GGA TGA GTA GTT ATG GTA / 417 / 1
SNP11793-R / TAA ACC AAA CAC AAC CCA CTT
SNP47086-F / TTG GGG ACT TAA CTC TCA CTC / 500 / 0
SNP47086-R / CAC TTC ACA ACA TAC AAA GAG G
SNP47144-F / GCT TTG GCG TTT GAC AC / 423 / NS
SNP47144-R / AAG CTA CAG GTT CAT ATA CAT C
SNP47244-F / ATA GGG AAG AAG AGT GCA CAT G / 276 / 0
SNP47244-R / ACC ATA TTC TCG AGT GGA ATC C
SNP47258-F / TAC ACC CTG AAT CTA GTA TTA C / 352 / 0
SNP47258-R / AGG CTC ATT GTG CAT CC
SNP50189-F / CCC GTA AAG AGA TGA AAG TCA TG / 554 / 0
SNP50189-R / GGT CAA CCA TAT GTC TGC TCA AT
SNP55067-F / GCT AAA GTC CAT TCT GCT TCA TTT / 448 / 0
SNP55067-R / ATT CAG GAG TCA GGA CGC ATA TA
SNP57559-F / ATA TAA TTG ACC AAG GCC CAA CT / 469 / 0
SNP57559-R / GCG GGA GCT AGG ATA GAA TAC AT
SNP57953-F / TGA GAT TTG CTC AAT GGT ATG TG / 235 / 0
SNP57953-R / AAA CGC CCG ATT AAT AGA ATT GT
SNP62601-F / TGG ACT TGT GGT CTA ATT CCA CT / 361 / 0
SNP62601-R / AAG GAT CTT AAT GGG ATT GTG GT
SNP62745-F / CTC CTA ACA TGG TGT CAA GAA CC / 371 / 1
SNP62745-R / ATA AGA GGC AAA ACC AAC CCT AG
SNP65415-F / ACC ATC AGT GTA GTC CTC CAA GA / 353 / NS
SNP65415-R / CAA TGT TAG ATT TGG CCT CTC AC
SNP65417-F / AAA GGA ATT CGG TTT GGA TAA CT / 308 / 0
SNP65417-R / CAT TGG ATG GTC AGT GGA ACT AT
cqOle-006 on Gm19 (LG-L)
SNP11641-F / AAG CAC ATG TTA TTA AAG CAC C / 409 / 0
SNP11641-R / CAT TTA GTT GTA AGG GAT GGA TTA GA
SNP14885-F / GCG GGC AAG CAA AGA CAA ACT ATA TG / 962 / NS
SNP14885-R / GCG GAG GAT CGG CGG AAA ACA AGT
SNP44913-F / GTC TAC ATT CCT AGT TTC CTT CG / 504 / 1
SNP44913-R / CCC AAT TTC CGC TTT CTA TTA GTT A
SNP47494-F / TTT CTC TTC CCA TCT CAT TTC C / 542 / 0
SNP47494-R / CTT GGT CGA TAT GTG CCT AAC
SNP47496-F / CAA ATG GAC CCT ATA TTA CCA C / 480 / NS
SNP47496-R / GAT GGG AGG GCG TAT CTG
SNP47498-F / TAT CTC TTG TCC TCC TCG TTT C / 503 / 1
SNP47498-R / TCC ATG CAC AAT CTT CCT CTC
GACGGACTTCCCAAACCAAACGTTAAACAATACTTGTTTTATTTTATTTTAATTTACTCTTCTTTTCCATTAGTTCTTTCTTCCACTGATAATCCAAAGTTCCCTCCCTTACTTTAAATCA[G/A]CAAACATCTCAAGAGAGTGTGTGTGAGAGATGGCAAGCATTGCTGGTACATGTCCTTTAGGAGCATTGCTCAGAAACATCTCAGAAAACAATGGGAAGATATCTGTCGTTCATTATGAGGGGCTTAGATTGCAGCAGAGACTACAAATGCATTCGCCAAGTCACTACATTTCAGCTTCTTCATGTAAGAGCCATTACTCTTTCCTATTGGTACTTGTACTTTTGCTTAATT[G/A]TATATGAAAGGAAAACCTTGATGACTAGTAAGGGCATG[T/A]TTGTATTGAAAATGTAAACGTGGTAGTGTGAATTCCAATGTCCTTGAAAATGTATATTGGAG[A/G]GAACTAATTACTTGAATGATTAATTGCTAAAATTTATCTCAATTTCACTTCATGTGATG[T/C]TGA[T/C]GTAGACGTATA[./T]TTTTTTTATCAATAAATATTAATTGCTAATTTTTGT[T/C]AGTGGGAATTC[G/A]AATCCACCACCTAGGGATGTAA[G/C]TGATCCGGGTTGAATTGAAATTTGTTTATTTTTCAATTTAATTCAATTAAATTTAATTGGGTTAGATTAGATTGTGTATATCTTTTTTATTTTGTCATTCTAATCATTAAAAAATCATGACCTTTTAAGTTTTAGAAAAAAATTAAAGAAATTCTCAAATTCAAAAATAGTAAAGATATTTTTAAAAATTAATATTCATAATAATTTATACTATACATATGATAAACTAAAAAAAAATGATAAATATATACTCACAAAATAAAATGGTCACCTAATTCAATTTTAATTAGGTTCAATAAACTAAAATCAATCTAATCTAGTAGCTCAATTCAACCCATAAAAATCTAATTGGGTTGGATTAGATTGGATGGATTCACCGATTGGATCTGATCCACTTACATCCCTATTGCCACCTCTCCTCCCTTTCCTTCTCCCTTCACCGTTAGGCCAACCCTACGTCTCCTTGCCGTGTATGTTGTGTTTGTATGTTAGGGACTGCCAATAAATTAGATTCTCAAATGCAGATCGTGAATTAGAAGAGTTTTCTGTTTTCTCTTTTTGTTGGATAGGTAAACATAGTGTATCTAAGATTAATATTTCATTTATCATGTTAGCCACAGACTGTTTAAACGGTGTAGTCTTTTTTTTTTTTTAATGTCTATTTCTTCTGCTTTGAATGTGAGCTATTCTACATCCTATTACCAATCTCATCCTACCCATAGTACTAACAGGTTTTGGAACTTCTTATAGAACTAATAATCAACAATTCTGTAATACTGATCAATTAGGTGAGCCTAGCAACTCGCCCATGTTGGCCTTTGTTCACGCTCTATAGGAAGTAGTACTTCTATTGATTGCCATACCAATATTCGGAAGGATAAAGTAAGTAATGATGATTATTGTGAAAAAATCTAACAGTTGGTGTTCTAATGTATTTATGATTTATGTTATGTATGTAAACATAATATTAAATCAATGTTGACAGTGATTACTTATTTTAGCTTCTAAAGGGAATTGTTTGCTCTAGATGAGTCAAATCCCAATATTCCATGGGTAACCGTAAATATCAGGACTTAAACTAGAATGTAAACATAATATTAAATCAATGTTGACAGTGATTCTAAAGGGAATTGTGGATTGAAGCTTTCACCCTTGCACTTGCAGCAGCTCCAAGATGCAGAACAATCAAGGTCATGGCTTCACCAACTGTTGCAGCACCAAAGAGAGAAAAGGATCCAAAAAAGCGAGTAGTAATAACAGGAATGGGTCTTGTGTCTGTCTTTGGTAGTGACATTGATGGCTTTTACAACAAGCTTCTTGAGGGAGAAAGTGGGATAAGCCTAATAGATAGGTTTGATGCCTCAAACTTCCCGGTCCGTTTTGGAGGTCAGATACGTGATTTCTCTTCAGAAGGCTACATTGATGGCAAGAATGACCGGCGCCTTGACGATTGCTGGAGGTATTGCATTGTTGCAGGCAAGAGGGCCCTTGATGATGCCAACCTTGGACAGCAAGTCCTGGACACTGTAAGTGACAGAATTTTTTGAAGCTGCATTTGTAGTTGCCTAACTAATGAAGAAAATTCTGATTGTATCAATTTAATTAATTTGATTGTATTTAAATTCTTAGGTTCACTAACCATATCAGTAGTGA[C/T]TGATTTGTTGTAAGGACAAATCTAGATACAGTTTCAAAGGTGTTGTTTGTGTTGTTTTTCCGATTAGAATTG[G/C]AGTTTTACCTTAGTAATTCACATAATAAAGGTTTGCATTAGAAGATTCGATAGATTATT[G/A]AGGTGAAACTCCAATTTTAATTGAAGAAAAGTACAAACAACACCTAAAGAAAGAACAGTATATAAGTTTTGTCCATGTTGTAATCATTAGAACAATGATTTAATAAGGAGGATTGATACGGAATTATACATTCATTATGTTTCTAGCAGATGGACAAAACAAGAATAGGAGTTCTGGTGGGATCAGGAATGGGAGGTATAACGGCTTTCTCTACTGGTGTGGAAGCTCTTGTACAGAAGGGGTATAAGAAAATTACTCCATTTTTCATTCCCTACTCCATCACCAACATGGGTTCTGCCTTGTTGGCCATAGACACAGGCCTAATGGGTCCCAATTATTCCATTTCCACTGCTTGTGCAACGGCGAATTACTGCTTTTGTTCGGCTGCTAATCACATTAGAAAAGGTGAAGCAGATATCATGGTGGTTGGTGGAACTGAGGCTGCAATCATGCCTAGTGGTCTTGGAGGCTTCATTGCTTGCAGGGCTTTGTCTCACAGGAATGAAGGCCCCAAGAAGGCTTCAAGACCATGGGACAAAGATCGTGATGGTTTTGTGATGGGTGAAGGCTCTGGTGTGCTGGTAGAACCTTGAACCCAAAATTTTCTTTTACACCATATGCATCTTTTGCTAGCAATTCTAAAGCAATATGGCTTAACAAGTTTTTGTTTTAACTTACTTTTGTTCACCTAATGACAACTTGATTCTCTATCACCGGAAAAAAAAAAAAGGTAATGGAGAGCTTGGAGAGTGCAACAAAAAGGGGAGCCAGAATAATAGCAGAATATTTGGGGGGTGCCATAACATGTGATGATCATCACATGACTGATCCAAGAGCTGATGGGCTTGGAGTTTCATCTTGCATAAGCAAGAGTTTAGAAGATGCTGGAGTTTCCCCTGAAGAGGTAAGAAATAGAGAGTATTACCAAGTAACCAGTATCAGAACTGAACTGATATATATATATATATATATATATATATATATATATATATATATATATACTCCTTTAAAAACAATTCAGATTCTTACGTTCAATTGTTTATGTAAAATTGATGAATTCTAATTGGCTTGGCTAACCTCTAAACTGTACAGGTAAACTATGTGAATGCTCATGCCACATCAACACTGGCTGGTGACTTGGCTGAGGTTAATGCAATTAAAAAGGTTTTTAAGGACACGTCAGAATTGAAAATGAATGCAACTAAGGTAAGAAACATTTTTTTTAGTTTTAAATACTTCAGAATCTTAACAGCGTTCTTCTGTCAACCTTGAAGTTATCATTATATTTTCTTATCGTTTCAGTCAATGATTGGTCATGGTCTTGGGGCTGCTGGTGGTTTGGAAGCCATAGCAACAATCAAAGCCATAACAACTGGCTGGTTGCATCCAACCATTAACCAAGATGTAACGTCCTGAACTAGAAATTCATCTTAACCTATTAATCAAATGTTAAACTTTGTAACTTTTGTTGATTCCCACAACATTTTAATATTAAGCGTCTAAGTTTCTCACTTTTCCACAGAACTTGGAGGCTAGTGTTACCATTGACACTGTCCCTAACGTTAAGAAGAAGCACGAAGTGAATGTTGGTATGTATTATCTCTTCAATCGAATAGAGGTAATAATGTAAAATTCATCGTTTCTGATCTTTAGTGTAATCCCTGCAGGTATATCCAACTCATTTGGATTCGGTGGACACAATTCAGTAGTTGTCTTTGCCCCATTCAGGCCATAAGTAGAATTCAAACTCAACCTGAGTATTTCTTTTACTTTGTC[T/.]TTTTTTTTTTCTTTACACATTTTGAGAACCAGTGATTTTTGGACACAAATTCATAGGACTATATGAGTTATGGATGGAAAATAGTTGTTTCGTATGTATTGAAATCATATACCCAGATCGTGTGGTCTAATGTAATGAGCCCTGAAGACTGAAGTCAATAAAATATAATTTTCAGT
Supplemental Fig. 1. Genomic sequence of putative KAS I gene associated with cqOle-001 on Gm05 (LG-A1) and location of SNPs between N00-3350 and Boggs. Sequences corresponding to exons are indicated with a gray background, intron sequences are shown with no background, 5’-UTR sequences are indicated in yellow background, and 3’-UTR sequences are indicated in pink background. SNPs are indicated in parenthesis (allele of N00-3350/ allele of Boggs).
AGAACAATCAAGGCCATGGCTTCCCCAACTGTTGGAGCACCAAAGAGAGAAAAGGATCCAAAAAAGCGGGTAGTAATAACAGGAATGGGTCTTGTGTCAGTCTTTGGTAGTGACGTTGATGCCTTTTACAACAAGCTTCTTGAGGGAGAAAGTGGGATAAGCCTAATAGATAGGTTTGATGCCTCAAACTTCTCGGTCCGTTTTGGAGGTCAGATACGTGATTTCTGTTCAGAAGGCTACATTGATGGCAAGAATGACCGGCGCCTTGACAATTGCTGGCGGTATTGCATTGTTGCAGGCAAGAGGGCCCTTGATGATGCCAACCTTGGAAAGCAAGTCCTTGACACTGTAAGTCACAGAATTTTTTGTAGCTGCATTATGTAGTTGCCTAACTAATCTAGAAAATTCTATTCTTAGGTTCACTAACCATATCAGTAGCGATTGATTTGTTGTAAGGACAAAATTTAGATACAGTTTCATAGGTGTTTTTTTTTTTCAATTAGAGTTGGAGTTTTACCTTAGTAATCAATGTAATAAAGGTTTGTATTAAAAGATTATCAAGGTGAAACTCCAATTTTAATTGGAGAAAAGTACAAACAACACCTAAAGAAAGAACTATATATAAGTTTTGTCCATGTTGTAATCCTTAGGACTATGATTTAATGAGGAGGATTGGTACGAAATTATACGTTCATTATGTCTCTAGCAGATGGACAAAACAAGAATAGGAGTTCTGGTGGGATCAGGAATGGGAGGTATAACG[A/G]CTTTCTCGAATGGTGTGGAAGCTCTTGTACAAAAGGGATATAAGAAAATTACTCCATTTTTCATTCCCTACTCCATCACCAACATGGGTTCTGCCTTGTTGGCTATAGACACAGGCCTAATGGGTCCCAATTATTCAATTTCCACTGCTTGTGCAA[T/C]GGCAAATTACTGCTTTTGTGCGGCTGCTAATCACATTAGAAAAGGTGAAGCAGATATCATGGTGGTTGGTGGAACTGAGGCAGCAATCATGCCTAGTGGTCTTGGAGGCTTCATTGCTTGCAGGGCTTTGTCTCACAGAAACGAAGACCCCAAGAAGGCTTCAAGACCATGGGACAAAGATC[A/G]TGATGGTTTTGTGATGGGTGAAGGCTCTGGTGTGCTGGTAGAAACTTGAACCCAAAATTTTCTTTTACGCTATATATACATCTTTTACTAGTAATTCTAAAGCAATATGGCCTAACAAGTTTTTGTTTTAACTTACTTTTGTTCACCTAATGACAACTTGATTCTCTAT[A/C]ACTGAAAAAAAATTGGAGAGTGCAACAAAAAGGGGAGCCAAAATAATAGCAGAATATTTGGGGG[./G]TGCCATAACGTGTGATGCTCATCACATGACTGATCCAAG[A/C]GTTGATGGTCTTGGAGTTTCATCTTGCATAAGCAAGAGTTTAGAAGATGCTGGAGTTTCTCCTGAAGAAGTAAGAAATAGAGAGTACTACCAACTACCAAGGCACAAGTATCCAAACTGAA[A/C]TGATCGATACATAATTACACATAATTAGAATGTTGCTTTGAAAAAGTTTAGATTCTATATTCAATTGTTTATGTAAAATTGATGATTTCTTATTGGTTTAATTTCTAAATTGTACAGGTGAACTATGTGAATGCTCATGCCACGTCAACACTGGCTGGTGACTTGGCTGAGGTTAATGCAATTAAAAAGGTTTTTAAGGACACATCAGAATTGAAAATGAATGCAACTAAGGTAAGAAACATTTTTTTTTAGTTTTAAATGCTTCATAATCTTATCGA[C/T]ATTCTTTCATTAACCTTGAAGTTTATCATTATATTTTCTTATCGTTTCAGTCAATGATTGGTCATGGTCTTGGGGCTGCTGGTGGTTTGGAAGCCATAGCAACTATCAAAGCCATAACAACTGGCTGGTTGCATCCAAGCATTAAC
Supplemental Fig. 2. Genomic sequence of putative KAS I gene associated with cqOle-003 on Gm18 (LG-G) and location of SNPs between N00-3350 and Boggs. Sequences corresponding to exons are indicated with a gray background and intron sequences are shown with no background. SNPs are indicated in parenthesis (allele of N00-3350/ allele of Boggs).
TACTGTTCTCAGTTCCCACCCATCACTTCACTTCCTTTCATCCAAACTCTTTTGCCTCTCTCTCTCTCTCTCTCTCT[GTC/CTCTCTC]GTGTTTTTGATTTGAGAATTGAGATCGAGTCTTTTGAGACATGGCATCCTCGTTGGCACCAGCTACCAAAGCCGCCACTAATTTGCGCCTCACACACTCTCTCCGCTTCTCTCCTAAACCCAACAACCTACGCTTTGCCACCAAGGTTCGTTACTGTTAGCAGTGTTCTTTCTCTCTTTATTTTAGGTCGCAACCTCTACCTCTACCTTACCTTAACCCTTGCTTTCCATTTTTCATACAATAATGTTCCAAATTACACACCACACTTTTTTAAATATGTATCTTCCATAGCTATAAAAACAATAAACACATCACATTTTGTTTTATTCATTTTATTGTTGCATGTGCAGCCTGGTAATACGCTGTTATGCACGAGGGTTAAGGCCCAATTAAATGAGGTAAACACTCTCTTCCCTTCACTTCTACATTATGTCTTTCGTATCTA[T/A]GTTTGTTCTTAAATGTGAATTTGGTGAAGAGGGCAACTTAGAGGGAAAATTGTGGATTTATAATCATTGATTGGAAAATTAACAATTGAGACTTTGATACATTTGGACTCTGTTTGGATAAACTTCTTCATACGTACTTATAGAGAAGAAAATGAA[T/C]TTAGCTTATCCTGTAAGCTAAAATCAACTCTGCAATCTAATTTCTCCAAAACCTGAGATGCATAAGTTGATTTTAGTTTAATCCATTTTACCTTTTTAATTTCTTCCTCAAGTGTTTTTGAAGAAGTTCATCCAAACATCACTAGAATGTGTAAACTTGTTAAAATCTTCTCCT[G/T]TGACACACATATTGGTCTCATCTTTTTGGCTTCAACAAGGTTGCCCTTGATAGTTCCTCCAATGCTACTTCTCCTCCTATGAAAGCCAAATCAAAGGAGGAACCACCCGCAAAGCCTTTAGCAGAACCATCTTCTTCAGTGTTGGCAACTCAAGAATCGGTCTCTCAGTTTATTACTCAAGTTGCAAGTCTTGTCAAGTAAGCAGATGCTTAAGTCTTGTTTCCTTCTATTAGTAATAGCAAATGTATAAGCTTTTTGCTGCATGATTGGAGTGGAGCAATCA[G/A]CTATGAAAAACTTCATGTCCAATTTTCTCCTGCAGGCTTGTTGATTCAAGAGACATTGTGGAGTTGAAGCTGAAGCAGCATGACGTTGAAGTAACAATCAGGAAAAAGGAGGCTATGCCTCAGCCACCACCTGCTCCTCAACCTTCTGTGGTGTATTCACCCCCTCCACCAGCGTTGCCACCACCACCTGTACCAGCATCTACTCCAGCACCTACTCTAGCTCGT[G/C]CAACCCCTACACCAACTTCAGCCCCTGCTGTGAAGTCAGCTAAATCATCACTTCCGCCTCTTAAAAGCCCCATGGC[A/T]GGGACATTCTACCGAAGTCCTGCACCTGGTGAACCTTCCTTTGTGAAGGTACATTTTATCTATTATATAGTTTTACATTTTTGTGTCATGCTTTTTTGAAACCTAATGCGAAGTATTCTTCCTTTAGGTTGGAGACAAAGTAAAGAAGGGGCAAG
TTGTATGCATCATTGAGGCAATGAAATTGATGAATGAAATTGAAGTAAGTTCAAAGGTTTTATATTTATTTTTATTGGTAACTGTTGATTAAAACAGGGGATTCTACATGTTATACCTTTATGTGGGCACTTATTTGATGCATCTACTGCCTCTTTTCATTTCTGTTTGCATTATCAAAATGTTGAATTTTGTGATCTAGAGTCTAGAACTTGTACCACAGAATGAGGTTAGTTTTATTAGCATTTTCCCTTTTTTCTATGCTCATATTTGTGATTCTTTTTTGTGAGGATTATGTTACTTAACATTTATTTATGTGATATAAAACATTAATTAGTGTAAACCACAAAAAAGAGTTTCTTTGTGCAAAATAAATCTTCATTAAATTCTCAATTGATTGTTAGCAACAGATGATGCAGCAGAATGTTAAAATAGAGAACATTACTCATTACATGACTTCCTTATTAAGTAAATTATGCCAATGGAAATGGAACATCAAAGATGTTAACATGTGTCAAAGAAGACGTTGTTAGCATTCTTAATATGAAAGTGCTTTTGCACTCCTGGGTTTGTCTAGCAAGGGAAAAGCTGAAAGAAACCATTTGAAGAGCACTGGGATGAAGCCATGAATCCCCTTTGGAGTTTTTCTTATTCATATCTTTAAAAAATGGGAAATAAGGTAAAAACAAAGTGACATTTTTGTAATTATCAGTAAAAATGAGAAATGAAAACAAAAAACATTTTTTTCAAACCAAATAGACCCCTTCATTTGTCTAACAAAGGAAAAGCAGAAGGAAACCATGTGAACATCACTGTGATGAAGCCATGAAAACAAGTCTATAACAACTCAAGTGTAGGGGTAAAGATTTTTACCAAAAAATCTAAATTCCAATTCAGCCAAAGACACCACTGAACATCATATGAAACACACTACATAACATCATAATAGGGGATGAGGGTGAAATTAAGCAGACTGTTGGATATGCAGAGGTAGAGGACGAGCATTTGATATTAAAGGATGAGGATGAACTAAGTGATATTTATCTTGGGAATGGTGCCGAATAGTTAAATTATGCATTAGGATCTAAGCATAGGAATTTGCATTTTGCCATCTTATTGATATCCTTTTTAGGTGTTGAAGACTTAAACTTTGTGCAGCTAGTCCCTATTTTTTGTCATTTTGATAGATGGTTTGAATCTTGGATTATTTTGAAGTTCATTTGTGATATATCAGCTTATGTTTCAGTTTATACAATGATGTATTCTCTTGTTTGACTTAATAATACTAATATCTTCCTCTTAAAAAAAATAATACTATTATCTTTATGTGAAAATATATTTTTTGTTATTTTTATTTATTGATCATGTATGTTATGAACTTATGATTCATAATAAGATTCAATTCACGTTTCGGAATGTAGGTCTTCTGATTCACAAGTCGAATCTCAATTAGATAACCTTCCTCTAGTCTGTCTGTTATTCAGAATCAATCCATATCAGTTTACCTTTCACTTCTGCTTCAATGGTTTCAGACTTCACAATAGGACAGATATGGATTTTTCCTTTTTCATTGGATGCCATGGTGTCACTATCTTCCCAATTGCTGAAGGTGGAAGTACTTCTGCTTGTGGCCACTCCAACAACGGAATTCCTGGTGTTTTTGCTTTAGTGGCTTCTGATCTGTTCTTGCTGGCTTGGATAATTGGGCGGTGATTCCTTCTTCTCCACAACCAATACCATTTCTTCAATTTGATCAAACTCTCTTTTTGCATCATCATTTTCCACATGTGTCTTACTGTTGACCTTGGTTATTGAGTGTTCCCCTTCAATCTCTACAATGATTTTGGATTGTTCTCCATTTTGCAGCTTATTTGTACCAATTCTTGTCTCTGATCATTTTAGACTCATTATAAAACATTGTTGTCTCTCCCAGATAGCAAGCATCCCAGTGAGCAAATGCTGCAGTTATCAAATCCTCCATAACTTTCCTTACTGCCAAGAGTATACATATTCATAATCTGAAATTTTTCACTGATCTCCCCTCAGATCCAGCAGGTTGGACCAATGCCAGGATCCCAGAAATTAGAACAGAAATGAGGGTTTTATTTAATAAAAAAACAGCTCAATCAGAAGATACATAAGAGGAATCACTCTCTCTCTAAGAGCAAAAGCATCTTGGAGAAAATACTCTCTCTACCCGGCCAAAAGGCCTAACCACCAAAATGAACCTCCTCCCCTGTATCACTCTAACCTTGCTCCCCTATATACGTTCTAACAACTTGCCAACTGAGCTGCAAAATGGAGTCCAATAGTTGTGCCACTTGACCCATTATCTAAACTGAACACTGTTACTGCAGCGTTAGGACCAAGGGTCCTATCATGGTTGATATCTCATTCATAAATGATAGGACCAAGGGTACTATCAGTAATTCAGTATTTAATTAGCTATTTAGGATTCAGAGTATTTTTGCAGTTTCACAAGTTAACATATATCTAGTTTTTCTGAAGATAGTGGCACTTCACGGTTCACACTCATATATACAATTTCCTGCTAGTAGTATATTCTTAATCCATTCTTTAGGAGAAAATAGCTTGTGAAATCAATTGGACTCATTACATTTATTTAGTTCAAAATAGCTACTCCTTACTGTTTTTGGTGGAGACGCAGCAGTGAAGTGAC[A/G]AAACTTA[C/T]TGAATTTTAAGGATGCATTATGGTTGACATTTCCATACAACAAACTTTTCATTAAACAAAATTGTATGCAATAAGCCTAATTTTGATGATTTGTCTCTTGGTTTGTAACTATGAGATGCAAATCAAAAGTCGAAAAGAAATGTTCAGGTTGTATCATTTTTTTAAATTGTCTAGATGGTCCTGTGGGTTATCTCTTCTATCTC[G/A]AAACTATAAAATTGTGTTCTATACAGGCTGATCAGTCAGGAACTATAGTTGAAATCGTTGCGGAAGATGCCAAGTCTGTAAGCGTTGACACTGTGAGTATATTCTAGGGCCTCCTGATTAATAACTCAACTGTTAAATTGACTTGCACTGTAGGTTTGACTTCTTTCGTTTCCTTGCAGCCCCTATTTGTGATTCAACCATAGAGCTCAGAGTATTTGAATAGTAGAGGAAGTGTAGGAAGATTTTGTTCTAAATTGGGATGACACAGTTGAGAAGAATTCTTGTTTACAATGGATTTATTCCTTATTGTTCGCTTCTACATGATTGCACTATGTTCTAGTGAACTTTTTTTTT[./T]GTTTCAACTTTCAGGCAAGTTAATGTGTATGATATTAACCTCAAGTAGTCTGTTTTTACTTCTTGTTTTGAAATGGTGAGGGAGCATTTGA[A/G]CCAAATTATCGTGTTTGGATATACTTTTATATAACACTTATAAGACAAGAAAATAAAAAG[T/G]TAATACAAATAGAGTTTTTCATATAAACTT
Supplemental Fig. 3. Genomic sequence of putative accB-1 gene associated with cqOle-004 on Gm18 (LG-G) and location of SNPs between N00-3350 and Boggs. Sequences corresponding to exons are indicated with a gray background, intron sequences are shown with no background, 5’-UTR sequences are indicated in yellow background, and 3’-UTR sequences are indicated in pink background. SNPs are indicated in parenthesis (allele of N00-3350/ allele of Boggs).