Hot Spots Forcarbon Emissions from Mediterranean Fluvial Networks During Summer Drought

Supplementary material for:

Article title

Hot spots forcarbon emissions from Mediterranean fluvial networks during summer drought

Journal name

Biogeochemistry

Author names

Lluís Gómez-Gener, Biel Obrador, Daniel von Schiller, Rafael Marcé, Joan Pere Casas-Ruiz, Lorenzo Proia, VicençAcuña, NúriaCatalán, Isabel Muñoz andMatthias Koschorreck

Correspondingauthor:

Ll. Gómez-Gener

Departmentd’Ecologia,Universitat de Barcelona, Barcelona, Spain

This section contains:

Online ResourceFig. S1. Examples of field flux measurements applying the chamber method in both aquatic and dry bed environments. Dimensions of both the aquatic and the soil chamber.

Online ResourceFig. S2. Spatial variations of CO2 efflux, CO2 concentration and O2concentration in the aquatic environments sampled along a longitudinal gradient from headwaters to the mouth of the Fluvià River.

Online ResourceTable S1. A data set of chemical, hydrological, and morphological parameters of the different studied sites. Detailed information about the analytical technique used for the determination of the reported parameters is described in the corresponding table caption.

Online ResourceFig. S1Examples of field flux measurements madein aan aquatic environmentand ba dry river bed. Dimensions of c the aquatic chamber and d the soil chamber.

Online ResourceFig. S2Spatial variation ofa CO2 efflux, bCO2concentration and c O2concentration in the aquatic environments sampled along a longitudinal gradient from headwaters to the mouth of the Fluvià River.

Online ResourceTable S1Location, surface water physicochemistry and hydromorphological characteristics of the studied sites

Strahler stream order was calculated with the Hydrological Extension in ESRI® ArcGISTM v. 10.0 software, data from a 2-meter digital elevation model (DEM)

Reported surface water physicochemical parameters are means of three measurements (n=3) at the same location where the flux measurements were carried out

Surface water temperature (Water temp.) and conductivity (Cond.) were measured with a portable probe (Cond 3310, WTW, Germany)

pHwas measured with a portable probe (pH 3110, WTW, Germany)

Oxygen saturation (O2) was measured with a portable probe (YSI ProODO Handheld, Yellow Springs, USA)

Dissolved organic and inorganic carbon concentrations in water (DOC and DIC) were measured from 0.45 µm-filtered water samples with a total organic carbon analyser (TOC-V CSH, Shimadzu, Japan). The samples for DOC determination were previously acidified to eliminate dissolved inorganic constituents

Ammonium concentration (NH4+) wasanalysed with ion chromatography (IC5000, DIONEX, USA) using an anion-exchangecolumn (IonPac® AS18, DIONEX, USA)Dissolved nitrite (NO2-), nitrate (NO3-) and phosphate (PO4-3) concentrations were analysed with ion chromatography (IC5000, DIONEX, USA) using a cation-exchangecolumn (IonPac® CS16, DIONEX, USA). Dissolved inorganic nitrogen (DIN) concentration is the sum of NH4+, NO2-, and NO3-concentrations.

The methodology used to calculate the hydromorphological parameters is detailed in the manuscript.

The size range of isolated pools is approximated.