1
CURRICULUM VITAE/
Prof. MARIA BOGOMILOVA ANGELOVA, D.Sc.
Address
/ The Stephan Angeloff Institute of MicrobiologyBulgarianAcademy of Sciences
Acad. G. Bonchev 26, 1113 Sofia, Bulgaria
Telephone
/ +359-2-9793126Fax
/ +359-2-800109Education
1965 - 1970 / Sofia University St. Kliment Ohridski, Faculty of Biology,Diploma in Microbiology and Biochemistry
1982 / The Stephan Angeloff Institute of Microbiology
BulgarianAcademy of Sciences
PhD in Microbiology
2003 / The Stephan Angeloff Institute of Microbiology
BulgarianAcademy of Sciences
D.Sc. in Microbiology
Career
/ The Stephan Angeloff Institute of MicrobiologyBulgarianAcademy of Sciences
Department of Mycology
1970 – 1976 / Microbiologist
1976 - 1987 / Assistant Professor
1987 - 2005 / Associated Professor
2005 - present / Professor in microbiology
1989 - present / Head of Department of Mycology
1992 - present / Member of Scientific Council of The Stephan Angeloff Institute of Microbiology, BulgarianAcademy of Sciences
2000 - present / Member of Bulgarian Society for Microbiology in Union of Scientists in Bulgaria
2005- present / Member of Balkan Microbiological Society
Teaching
1987-1988
/HonorAssistant in Sofia University St. Kliment Ohridski, Faculty of Biology, Department of Biotechnology
2003
/Lecture in 2nd International training course for young scientists “Molecular methods in Microbiology”, hold byInstitute Pasteur, Paris, France and The Stephan Angeloff Institute of Microbiology Bulgarian Academy of Sciences
2000 - present
/Current PhD projects
- Protective role of superoxide dismutase in Humicola lutea 103 against copper-induced stress
- Superoxide dismutase by Aspergillus niger: heat shock-induced biosynthesis and structural characterization
- Antioxidant defense of spermatozoa from species Ovis aries and Bos taurus during in vitro conservation
Work experience
Main scientific field / Microbiology, Mycology, Biochemistry, BiotechnologyMain research topics /
- Taxonomic identification of filamentous fungi
- Fungal morphology, physiology and biochemistry under normophysiological and stress conditions;
- Immobilized cells;
- Production of valuable enzymes
- Fungal biodegradation of natural and synthetic polymers; fungicides; methods for application of fungicides
Current research /
- Fungal cell response against different type of stresses such as oxidative stress, heat- and cold shock, stress of immobilization and heavy metal exposure; evaluation of oxidative stress biomarkers; antioxidant enzyme defence;
- Effect of stress factors on fungal metabolism;
- Adaptation to stress factors;
- Fungal cell factories for production of valuable biological-active compounds by free and immobilized cultures; cold-active enzymes; regulatory mechanisms in the biosynthesis; purification and characterization of enzymes;
- Antarctic fungi, cold-active enzymes;
- Production, characterisation and application of fungal SOD.
Research stays, abroad
1974 / Institute of Biochemistry and Biophysics, PolandAcademy of Sciences, Warsaw, Poland1977 / Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic
1982, 1985 / SkryabinInstitute of Physiology and Biochemistry of microorganisms, Russian Academy of Sciences, Pushchino, Russia
1999, 2000, 20001 / Institute of Physiological Chemistry, University of Tübingen, Germany
2002 / University of Padua, Italy
Main research projects(as Project Leaderor Supervisor)
International grants1999-2001
2000-2001
1998-2004
2001-2002
2004-2006 / Isolation, purification and medical application of superoxide dismutase from fungi (LST.CLG 973968, with Prof. W. Voelter, Tübingen, Germany), Science for Peace and Security, NATO;
Biotechnologische Production einer Cu/Zn-Superoxiddismutase aus dem Fadenpilz Humicola lutea, Aufklärung deren Proteinstructur und Untersuchungen zu ihrer biochemischen und medizinischen Applikation (436 BUL 113/112/0, Prof. W. Voelter, Tuebingen, Germany), DFG, Germany;
International Program „Bioconversion 2000“ Immobilization of microbial cells in polymer carriers (with Prof. B.A. Ustinnikov, Institute of food biotechnology, Moscow, Russia)
Recombinant glycosylated Cu/Zn-superoxide dismutase: production and structural characterization(LST.CLG.977860, with Prof. B. Salvato, Padua, Italy), Science for Peace and Security, NATO;
Superoxide dismutase by Aspergillus niger: heat shock-induced biosynthesis and structural characterization (LST.CLG 980520, with Prof. B. Salvato, Padua, Italy); Science for Peace and Security, NATO;
Bilateral projects
1999-2002
2006-2009 / Superoxidedismutasesfromfugi (Prof. W. Voelter, University of Tübingen, Germany)
Cold-adaptation of Antarctic fungi and their application (D-r S. Tosi, University of Pavia, Italy).
National grants
1989-1990
1989-1992
1989-1992
1992-1995
1994-1997
1996-2000
1998-2000
1998-2001
2001-2003
2002-2005
2003-2007
2003-2007
2004-2008
2006-2009 / Regulation of secondary metabolism in free and immobilized fungal cells (50/06/06);
Study of fungal metabolism and biosynthesis of biological active compounds (496/89);
Immobilization of fungal cells producing enzymes (1169/89)
Induction of SOD biosynthesis by filamentous fungi (К/225)
IsolationandcharacterizationofMn-SODfromHumicola lutea 110(Х/499);
Regulation of carbon metabolism in Aspergillus niger(К/618)
Production of superoxide dismutase by the fungal strainHumicola lutea 103 AL (5012)
Molecular structure, conformation stability and properties of Cu/Zn-containing superoxide dismutase by the fungal strain Humicola lutea 103(X-822, with D-r P. Dolashka);
Cu/Zn-containing superoxide dismutase by the fungal strain Humicola lutea 103: production and application in in vitropreservation of male gametes from farm animals (MU К-1004/01);
Modeling and optimization of biotechnological process for superoxide dismutase production by neural network modeling (MU-I-1202/02)
Fungal superoxide dismutase: adaptation to oxidative stress and conformational control of enzyme activity (К/1302)
Biological role of the antioxidant defense in filamentous fungi, isolatrd from Antarctica (B-1309)
Biological function of antioxidant enzymes in cell respons of Aspergillus niger against temperature stress (K-1401);
Metabolic cold-adaptation in Antarctic fung (VU-B-205/06, with Prof. I. Ivanova), The National Science Fund of Ministry of Education and Science
Industrial grants
1990-19911991-1992
1992-1993
2001-2003 /
Novel agents for conservation of wet leather
Developmentandintroducingofatechnologyforapplication of a new biocide in the leather industryElaboration of technology for enzymatic treatment of pig leather
Effect of Cu/Zn-SOD produced by Humicolalutea on sperm function and antioxidant defense of Bosstaurus in invitro storage (with Prof. Z. Zachariev);
Reviewer in the following refereed journals:
/ Enzyme and Microbiological Technology; Biotechnology progress; Journal of Food EngineeringScientific papers
/ 92Book chapter
/ 1Translated book
/ 1Patents
/3
Introducing of industrial-scale technology
/5.
Citations
/ More than 160 known citationsComputerskills
/ MS Office; WindowsXP;Exsel, Origin; Photoshop; MS Power Point; Corelphotopaint; Coreldrow; home page editing; Internet ExplorerSELECTED PUBLICATIONS
Angelova, M., L. Genova, L. Slokoska, S. Pashova. Effect of glucose on the superoxide dismutase production in fungal strain Humicola lutea. Can. J. Microbiol. 1995, 41, 11, 978-983.
Angelova, M., E. Petricheva, L. Slokoska, Ch. Konstantinov, L. Genova, S. Pashova, P. Sheremetska. Immobilization of acid proteinase producer Humicola lutea 120-5 with photo-crosslinkable prepolymer. J. Biotechnology 1995, 39, 41-48.
Angelova, M., L. Genova, S. Pashova, L. Slokoska. Induction of superoxide dismutase synthesis in Humicola lutea 110 by pentachlorophenol. Can. J. Microbiol. 1996, 42, 1197-1202.
Angelova, M., L. Genova, S. Pashova, L. Slokoska, P. Dolashka. Effect of cultural conditions on the synthesis of superoxide dismutase by Humicola lutea 110. J. Ferment. Bioeng. 1996, 82, 464-468.
Angelova, M., E. Petricheva. Glucose- and nitrogen-dependence of acid proteinase production in semicontinuous culture with immobilized cells of Humicola lutea 120-5. J. Biotechnology 1997, 58, 51-58.
Angelova, M., P. Scheremetska, M. Lekov. Enhanced polymethylgalacturonase production from Aspergillus niger 26 by calium-alginate immobilization. Process Biochem. 33, 1998, 299-305.
Slokoska, L., M. Angelova. Immobilization of polymethylgalacturonase producing Aspergillus niger on Luffa sponge material. Z. Naturforsch.1998, 53C, 11/12, 968-972.
Slokoska, L., M. Angelova, S. Pashova, E. Petricheva, C. Konstantinov. Production of acid proteinase by H. lutea 120-5 immobilized in mixed photo-crosslinked polyvinyl alcohol and calcium-alginate beads. Process Biochem. 1999, 34, 73-76.
Pashova, S., L. Slokoska, E. Krumova, M. Angelova.Induction of polymethylgalacturonase biosynthesis by immobilized cells of Aspergillus niger 26. Enzyme Microb. Technol. 1999, 24, 535-540.
Dolashka-Angelova, P., L. Genova, S. Stoeva, B. Stefanov, M. Angelova, R. Hristova, S. Pashova, W. Voelter. Isolation and characterization of a novel superoxide dismutase from fungal strain Humicola lutea 110. J. Pept. Res. 1999, 54/4, 279-289.
Dolashka-Angelova, P.,M. Angelova, L. Genova, S. Stoeva, W. Voelter. A novel Cu,Zn superoxide dismutase from the fungal strain Humicola lutea 110: isolation and physico-chemical characterization. Spectrochimica Acta, part A 1999, 55, 2249-2260.
Pashova, S., L., Slokoska, P., Sheremetska, E., Krumova, L., Vassileva, M. Angelova. Physiological aspects of immobilized Aspergillus niger cells producing polymethylgalacturonase. Process Biochem. 1999, 35, 15-19.
Pashova, S., L., Slokoska, P., Dolashka-Angelova, S., Vassilev, P., Sheremetska, M. ANGELOVA. Effect of dissolved oxygen concentration on superoxide dismutase production by Humicola lutea cells. Z. Naturforsch. 1999, 54c, 1049-54.
Angelova, M., S., Pashova, L. Slokoska. Comparison of antioxidant enzyme biosynthesis by free and immobilized Aspergillus niger cells. Enzyme Microb. Technol. 2000, 26, 544-549.
Toshkova, R., P. Dimitrova, E. Ivanova, P. Dolashka, M. Angelova, S. Pashova, L. Slokoska, W.Voelter. Imunoprotective effect of Humicola lutea superoxide dismutase on myeloid Graffi tumor-bearing hamsters. Z. Naturforsch. 2000, 55c, 649-656.
Dimitrova, P. A., R. A. Toshkova, E. H. Ivanova, Z. H. Stefanova, M. B. Angelova, P. A. Dolashka and W. Voelter. Superoxide Production by Phagocytes in Myeloid Graffi Tumor-Bearing Hamsters. Z. Naturforsch. 2000, 55c, 799-805.
Angelova, M., P. Dolashka-Angelova, E. Ivanova, J. Serkedjieva, L. Slokoska, S. Pashova, R. Toshkova, S. Vassilev, I. Simeonov, H-J. Hartmann, S. Stoeva, U. Weser, W.Voelter. A novel glycosylated Cu/Zn-containing superoxide dismutase: production and potential therapeutic effect. Microbiology (UK), 2001, 147, 1641-1650.
Ivanova, I., M.Angelova,L. Slokoska, S. Pashova, R. Toshkova, P. Dolashka-Angelova, P. Dimitrova, W. Voelter, Effect of Cu/Zn-superoxide dismutase from the fungal strain Humicola lutea 103 on antioxidant defense of graffi tumor-bearing hamsters, Z. Naturforsch, 2002, 57c, 197-204.
Toshkova R., Ivanova E., Angelova, M.,Dolashka P., Voelter W. Effect of the fungal Cu/Zn-superoxide dismutase on the cell-mediated immune response in tumor Graffi bearing hamsters. Z. Naturf., 2003, 58c, 128-134.
Serkedjieva, J., I. Roeva, M. Angelova,P. Dolashka, W. Voelter. Combined protective effect of a fungal Cu/Zn-containing superoxide dismutase and rimantadine hydrochloride in experimental murine influenza A virus infection Acta Virol. 2003, 47, 53-56.
Stefanov R., Angelova, M., Stefanova Z., Subev M., Dolashka P., Voelter W., Zachariev Z. Cu/Zn-Superoxide dismutase from the fungal strain Humicola lutea 103 improves ram spermatozoa functions during liquid storage. Andrologia, 2004, 2, 51-56.
Dolashka-Angelova P., Stevanovic S., Dolashki A., Angelova M., Pashova S., Krumova E., Serkejieva J., Zacharieva S., Voelter W. Structural and functional analyses of glycosylated Cu/Zn-SOD from the fungal strain Humicola lutea 103, cultivated in copper stress conditions. Biochem. Biophys. Res. Com. 2004, 317, 1006-1016.
Angelova M., Pashova S., Spasova B., Vassilev S., Slokoska S. Oxidative stress response of filamentous fungi induced by hydrogen peroxide and paraquat. Mycol. Res. 2005, 109 (2), 150-158.
Sokmen M., M. Angelova, E. Krumov, S. Pashov, S. Ivanchev, A. Sokmen, J. Serkedjieva. In vitro antioxidant activity of polyphenol extracts with antiviral properties from Geranium sanguineum L. Life Sci. 2005, 76, 2981-2993.
Abrashev, R., P. Dolashka, R. Christova, L. Stefanova, M. Angelova Role of antioxidant enzymes in survival of conidiospores of Aspergillus niger 26 under conditions of temperature stress. J. Appl. Microbiol. 2005,99, 902-905.
Angelova, M., Doumanova, L., Galabov, A., The Stephan Angeloff Institute of Microbiology - center of Bulgarian microbiological science. Advanc Bulg Scie, 2005, 3-4, 24-38.
Gocheva, Y., E. Krumova, L. Slokoska, V. Gesheva, M.Angelova. Isolation of filamentous fungi from Antarctica. Compt. rend. Acad. bulg. Sci., 2005 58, 4, 403-408.
Abrashev, R., P. Dolashka, M. Angelova. Enhanced antioxidant enzyme activities in temperature-resistant variants of Aspergillus niger. Compt. rend. Acad. bulg. Sci., 2005, 58, 4, 417-420.
Elena Kovacheva, Milen Georgiev, Svetlana Pashova, Maria Angelova, Mladenka Ilieva Radical Quenching by Rosmarinic Acid from Lavandula vera MM Cell Culture Z. Naturforsch. 2006, 61c, 517-520.
Gocheva, Y., Krumova, E., Slokoska, L., Miteva, J., Angelova, M. Cell response of Antarctic and temperate strains of Penicillium spp. to different growth temperature. Mycol. Res. 2006, 110, 1347-1354.
Stefanov R., E. Krumova, M. Angelova, P. Dolashka, W. Voelter, Z. Zachariev Artificial Insemination of Sheep and Cow with Semen Treated by Cu/ Zn-superoxide Dismutase from the Fungal Strain Humicola lutea 103.2006, W J Zoology, 1, 1, 36-39.
Krumova, E., Dolashka-Angelova, P., Pashova, S., Stefanova, L., Van Beeumen, J., Vassilev, S., Angelova, M. Improved production by fed-batch cultivation and some properties of Cu/Zn-superoxide dismutase from the fungal strain Humicola lutea 103. Enzyme Microb. Technol.2006, (in press).
Krumova, E., Pashova, S., Dolashka, P., Stefanova, Tz., Angelova, M.Biomarkers of oxidative stress in the fungal strain Humicola luteaundercopper exposure. (submitted)
Krumova, E., Dolashki, A., pashova, S., Dolashka-Angelova, P., Stevanovic, S., Hristova, R., Stefanova, L., Voelter, W., Angelova, M. Unusual location and characterization of Cu/Zn-containing superoxide dismutase from filamentous fungus Humicola lutea. (submitted)