Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Manuscript title-Synthetic peptides are better than native antigens for development of ELISA assay for diagnosis of Tuberculosis
Journal name-International Journal of Peptide research and Therapeutics
Author names- Arti R. Mishra1, Vinita R. Hutke1, Ashish R. Satav2, Syed A. Ali1,Hatim F. Daginawala1, Lokendra R Singh1, Rajpal S. Kashyap*1
Affiliation
1Biochemistry Research Laboratory, Central India Institute of Medical Sciences, 88/2 Bajaj Nagar, Nagpur-440010, India
2Meditations, AIDS, Health, Addiction & Nutrition (MAHAN) Trust, C/O Mahatma Gandhi Tribal Hospital, Karmagram, Utavali, Tahsil Dharni, District: Amaravati, Maharashtra, India.
*Corresponding Author
Dr. Rajpal S. Kashyap
Address: Biochemistry Research Laboratory, Central India Institute of Medical Sciences,
88/2, Bajaj Nagar, Nagpur, Maharashtra, India
Telephone: +91-712-2233381/2236441; Fax: 0712-2236416
E-mail:
Supplementary information Fig 1Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-17
Sequence / IVMPVGGQSSFYSDWYS
Quantity / 10.0mg
Purity / 95.33%
Molecular Weight / 1923.1
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-17 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-23 14:25:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 4.0957015.05962083.1911.6816
2 11.8423178.04831695.5940.8585
3 12.2058936.64243860.7501.1880
4 12.460329297.0943519859.00095.3398
5 13.055666.3106563.6210.1778
6 14.6103084.41127846.3910.7543
Total 100.0000
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Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-17 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 1923.15 / Block temp： / 200℃ / Date/Time： / 2011-5-2314:42:20
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-18
Sequence / MILAAYHPQQFIYAGSL
Quantity / 10.0mg
Purity / 96.48%
Molecular Weight / 1923.2
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-18 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-23 15:25:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 11.430862.5568278.5020.2618
2 11.7952500.82215480.4520.4895
3 11.988274852.9383051202.00096.4832
4 12.3935442.08544741.7301.4148
5 12.7373131.84242714.9221.3507
Total 100.0000
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Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-18 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 1923.28 / Block temp： / 200℃ / Date/Time： / 2011-5-25 11:24:52
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-20
Sequence / TAASPPSTAAA
Quantity / 10.0mg
Purity / 97.04%
Molecular Weight / 944.0
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-20 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 14:28:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 11.9081741.30923874.3590.2342
2 12.3005129.24375489.4920.7405
3 12.67213960.924107853.5471.0580
4 13.022609292.0009892809.00097.0406
5 14.1823011.03546519.5390.4563
6 14.633788.78015343.4970.1505
7 21.1101690.39332614.4140.3199
Total 100.0000
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Page 1 of 11
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-20 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 944.02 / Block temp： / 200℃ / Date/Time： / 2011-5-25 11:20:49
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-21
Sequence / RPLVAPPPAPAPAP
Quantity / 10.0mg
Purity / 95.88%
Molecular Weight / 1350.6
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 11
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-21 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 15:28:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 7.7324590.23550294.0041.8971
2 8.557774.1387570.0000.2855
3 11.268338.7272824.3020.1065
4 11.7703244.85748398.8521.8256
5 12.447136510.4532542067.75095.8853
Total 100.0000
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Page 1 of 13
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-16
Sequence / HPRSLFPEFSEL
Quantity / 10.0mg
Purity / 95.28%
Molecular Weight / 1458.6
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 13
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-16 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-23 13:25:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 4.4421725.46811113.5970.3724
2 5.3751013.6006969.5940.2335
3 6.553836.5725803.2000.1944
4 8.3271394.88011872.0120.3978
5 11.6321816.13121056.0060.7055
6 12.2122276.94523773.9750.7966
7 12.4675834.79260239.7502.0184
8 12.788290930.1882843735.25095.2814
Total 100.0000
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Page 1 of 19
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-16 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 1458.65 / Block temp： / 200℃ / Date/Time： / 2011-05-25 10:54:44
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-22
Sequence / LKTQIDQVESTAGS
Quantity / 10.0mg
Purity / 95.46%
Molecular Weight / 1476.6
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 19
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-22 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 10:28:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 10.762916.07310255.9930.4696
2 11.662160672.0002084867.25095.4689
3 12.3581472.00016595.1970.7599
4 12.6231472.00016035.1930.7343
5 13.448556.7234726.3970.2164
6 13.913789.5307739.7260.3544
7 14.235529.7125312.5550.2433
8 24.3482979.97538284.8161.7531
Total 100.0000
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Page 1 of 19
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-22 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 1476.62 / Block temp： / 200℃ / Date/Time： / 2011-5-25 11:19:09
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-23
Sequence / QQQALSS
Quantity / 10.0mg
Purity / 96.73%
Molecular Weight / 760.8
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-23 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 11:28:25 / Analyst: / YF
Gradient : / 25%ACN-50%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time Height Area Conc.1 12.2003974.44050660.2622.3814
2 12.523173515.7662057842.12596.7329
3 13.592575.9997491.2070.3521
4 25.4321105.64511350.3960.5335
Total 100.0000
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-23 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 760.81 / Block temp： / 200℃ / Date/Time： / 2011-05-25 10:53:44
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-24
Sequence / IQGNVTSIHSLLD
Quantity / 10.0mg
Purity / 95.21
Molecular Weight / 1396.5
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-24 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 12:28:25 / Analyst: / YF
Gradient : / 18%ACN-43%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time HeightArea Conc.1 13.6722345.59429699.7950.5840
2 14.210414533.9384842800.00095.2190
3 15.0053078.67734933.9450.6869
4 16.7171907.79353585.6371.0536
5 17.6783102.935103606.7032.0371
6 22.0031727.99821334.3260.4195
Total 100.0000
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-24 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 1396.58 / Block temp： / 200℃ / Date/Time： / 2011-5-25 11:22:18
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
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Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Certificate of Analysis
Product / Synthetic PeptideCustomer P.O. / 6792
Our Ref. No. / GP1105139
Name / P1105139-25
Sequence / AYQGVQQ
Quantity / 10.0mg
Purity / 95.40%
Molecular Weight / 792.8
Appearance / White Lyophilized Powder
HPLC Result / Attached
Mass Spectrometry / Attached
All peptides are shipped as lyophilized powder. Store in a cool dry place. Store at -20ºC for long-term storage.For in vitro research use only.
For damaged package or vials, please inform us immediately upon receiving. All other claim should be made within 30 days when products arrive at the destination in writing form (fax or email) and failure to do so shall constitute a waiver by customer for any and all such claims.
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
UUHPLC Result
Sample No.： / C:\ DIONEX\peptide\P1105139-25 / Instrument: / DIONEX summitColumn: / 4.6*250mn,Venusil XBP-C18 / Volume： / 5ul
Mobile Phase / A:0.1%TFA in 100% water / Wavelength： / 220nm
B:0.1%TFA in 100% acetonitrile / Flow rate： / 1.0ml/min
Date/Time: / 2011-05-25 14:28:25 / Analyst: / YF
Gradient : / 10%ACN-35%ACN in 25 min
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ Results ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝
Peak No. Ret Time HeightArea Conc.1 11.2326853.79861865.6091.5645
2 12.0481309.83810460.7970.2645
3 12.3351097.1604235.3060.1071
4 12.513318137.6563772613.25095.4071
5 13.1109872.55682223.2032.0794
6 14.8522591.85022828.8130.5773
Total 100.0000
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
Page 1 of 28
Hongkong GenicBio BioTech Co., Limited
Tel: +86-(0)21-3770 9035︱Fax: +86(0)21-6085 3385︱Email:
Mass Spectrometry
＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝ ＝Sample Information Item
Instrument： / SHIMADZU LCMS-2011 / Probe： / ESI / Probe bias： / ＋4.5kv
Analyst： / Chao / Nebulizer Gas Flow： / 1.5L/min / Detector： / 1.5kv
Sample： / C:\LCMS-2011\method\peptide\ P1105139-25 / CDL： / －20.0v / T.Flow： / 0.2ml/min
Inj.Volume： / 1.000 / CDL temp： / 250℃ / B.conc： / 50%H2O/50%ACN
MW： / 792.85 / Block temp： / 200℃ / Date/Time： / 2011-5-25 11:15:35
Add: Room 301, Building 2, Meijiabang Road 1508, Shanghai, 201620 China
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